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Quantification of Lumefantrine in Human Plasma Using LC-MS/MS and Its Application to a Bioequivalence Study.

Pingale SG, Mangaonkar KV - J Pharm (Cairo) (2012)

Bottom Line: Artesunate was used as an internal standard for lumefantrine.The overall recovery for lumefantrine and artesunate was 93.16% and 91.05%, respectively.This validated method was used successfully for analysis of plasma samples from a bioequivalence study.

View Article: PubMed Central - PubMed

Affiliation: Analytical Chemistry Research Laboratory, Mithibai College of Arts, Chauhan Institute of Science & Amrutben Jivanlal College of Commerce & Economics, Vile Parle (W), Mumbai 400056, India.

ABSTRACT
An analytical method based on protein precipitation has been developed and validated for analysis of lumefantrine in human plasma. Artesunate was used as an internal standard for lumefantrine. Inertsil ODS column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves simple isocratic chromatographic condition and mass spectrometric detection in the positive ionization mode using an API-3000 system. The total run time was 2.5 minutes. The proposed method has been validated with linear range of 200-20000 ng/mL for lumefantrine. The intrarun and interrun precision values are within 6.66% and 5.56%, respectively, for lumefantrine at the lower limit of quantification level. The overall recovery for lumefantrine and artesunate was 93.16% and 91.05%, respectively. This validated method was used successfully for analysis of plasma samples from a bioequivalence study.

No MeSH data available.


Related in: MedlinePlus

Mean concentration versus time profile of lumefantrine in human plasma from sixty subjects receiving a single oral dose of 120 mg lumefantrine tablet as test and reference.
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Related In: Results  -  Collection


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fig4: Mean concentration versus time profile of lumefantrine in human plasma from sixty subjects receiving a single oral dose of 120 mg lumefantrine tablet as test and reference.

Mentions: The mean concentration versus time profile of lumefantrine in human plasma from sixty subjects that are receiving a single oral dose of 120 mg lumefantrine tablet as test and reference is shown in Figure 4.


Quantification of Lumefantrine in Human Plasma Using LC-MS/MS and Its Application to a Bioequivalence Study.

Pingale SG, Mangaonkar KV - J Pharm (Cairo) (2012)

Mean concentration versus time profile of lumefantrine in human plasma from sixty subjects receiving a single oral dose of 120 mg lumefantrine tablet as test and reference.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4595938&req=5

fig4: Mean concentration versus time profile of lumefantrine in human plasma from sixty subjects receiving a single oral dose of 120 mg lumefantrine tablet as test and reference.
Mentions: The mean concentration versus time profile of lumefantrine in human plasma from sixty subjects that are receiving a single oral dose of 120 mg lumefantrine tablet as test and reference is shown in Figure 4.

Bottom Line: Artesunate was used as an internal standard for lumefantrine.The overall recovery for lumefantrine and artesunate was 93.16% and 91.05%, respectively.This validated method was used successfully for analysis of plasma samples from a bioequivalence study.

View Article: PubMed Central - PubMed

Affiliation: Analytical Chemistry Research Laboratory, Mithibai College of Arts, Chauhan Institute of Science & Amrutben Jivanlal College of Commerce & Economics, Vile Parle (W), Mumbai 400056, India.

ABSTRACT
An analytical method based on protein precipitation has been developed and validated for analysis of lumefantrine in human plasma. Artesunate was used as an internal standard for lumefantrine. Inertsil ODS column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves simple isocratic chromatographic condition and mass spectrometric detection in the positive ionization mode using an API-3000 system. The total run time was 2.5 minutes. The proposed method has been validated with linear range of 200-20000 ng/mL for lumefantrine. The intrarun and interrun precision values are within 6.66% and 5.56%, respectively, for lumefantrine at the lower limit of quantification level. The overall recovery for lumefantrine and artesunate was 93.16% and 91.05%, respectively. This validated method was used successfully for analysis of plasma samples from a bioequivalence study.

No MeSH data available.


Related in: MedlinePlus