Limits...
Genome sequencing reveals a new lineage associated with lablab bean and genetic exchange between Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans.

Aritua V, Harrison J, Sapp M, Buruchara R, Smith J, Studholme DJ - Front Microbiol (2015)

Bottom Line: This revealed considerable genetic variation within both taxa, encompassing both single-nucleotide variants and differences in gene content, that could be exploited for tracking pathogen spread.The strains from lablab represent a new, previously unknown genetic lineage closely related to strains of X. axonopodis pv. glycines.Finally, we identified more than 100 genes that appear to have been recently acquired by Xanthomonas axonopodis pv. phaseoli from X. fuscans subsp. fuscans.

View Article: PubMed Central - PubMed

Affiliation: International Center for Tropical Agriculture Kampala, Uganda.

ABSTRACT
Common bacterial blight is a devastating seed-borne disease of common beans that also occurs on other legume species including lablab and Lima beans. We sequenced and analyzed the genomes of 26 strains of Xanthomonas axonopodis pv. phaseoli and X. fuscans subsp. fuscans, the causative agents of this disease, collected over four decades and six continents. This revealed considerable genetic variation within both taxa, encompassing both single-nucleotide variants and differences in gene content, that could be exploited for tracking pathogen spread. The bacterial strain from Lima bean fell within the previously described Genetic Lineage 1, along with the pathovar type strain (NCPPB 3035). The strains from lablab represent a new, previously unknown genetic lineage closely related to strains of X. axonopodis pv. glycines. Finally, we identified more than 100 genes that appear to have been recently acquired by Xanthomonas axonopodis pv. phaseoli from X. fuscans subsp. fuscans.

No MeSH data available.


Related in: MedlinePlus

The SPI-1-like T3SS in Xap GL 1. The figure shows a TBLASTN alignment of the X. albilineans chromosome (Pieretti et al., 2009) vs. the genome of Xap NCPPB 3035 (sequenced in the present study). Sequence identity is indicated by the black-gray-white color scale.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4595841&req=5

Figure 8: The SPI-1-like T3SS in Xap GL 1. The figure shows a TBLASTN alignment of the X. albilineans chromosome (Pieretti et al., 2009) vs. the genome of Xap NCPPB 3035 (sequenced in the present study). Sequence identity is indicated by the black-gray-white color scale.

Mentions: A previously published suppression subtractive hybridizations study comparing bean pathogens and closely related xanthomonads revealed the presence of genes encoding several protein components of a T3SS similar to that of Salmonella pathogenicity island 1 (SPI-1) in the genome of Xap CFBP 6164 (Alavi et al., 2008). This strain is synonymous with NCPPB 1811 and belongs to lineage GL 1. Subsequently, genome sequencing revealed that X. albilineans encodes a SPI-1-like T3SS (Pieretti et al., 2009, 2015) and targeted sequencing confirmed its presence in two further Xap GL 1 strains: CFBP 2534 (same as NCPPB 3035) and CFBP 6982 (Marguerettaz et al., 2011). Whole-genome sequencing in the current study indicated that this SPI-1-like T3SS was encoded in the genomes of all GL 1 strains from common bean and Lima bean (Figure 8) but was absent from GL fuscans and from the lablab-associated strains. All of the putative structural genes for the T3SS are conserved in Xap GL 1 but the xapABCDEFGH genes, hypothesized to encode effectors that are substrates of the T3SS in X. albilineans (Marguerettaz et al., 2011), are not conserved in Xap.


Genome sequencing reveals a new lineage associated with lablab bean and genetic exchange between Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans.

Aritua V, Harrison J, Sapp M, Buruchara R, Smith J, Studholme DJ - Front Microbiol (2015)

The SPI-1-like T3SS in Xap GL 1. The figure shows a TBLASTN alignment of the X. albilineans chromosome (Pieretti et al., 2009) vs. the genome of Xap NCPPB 3035 (sequenced in the present study). Sequence identity is indicated by the black-gray-white color scale.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595841&req=5

Figure 8: The SPI-1-like T3SS in Xap GL 1. The figure shows a TBLASTN alignment of the X. albilineans chromosome (Pieretti et al., 2009) vs. the genome of Xap NCPPB 3035 (sequenced in the present study). Sequence identity is indicated by the black-gray-white color scale.
Mentions: A previously published suppression subtractive hybridizations study comparing bean pathogens and closely related xanthomonads revealed the presence of genes encoding several protein components of a T3SS similar to that of Salmonella pathogenicity island 1 (SPI-1) in the genome of Xap CFBP 6164 (Alavi et al., 2008). This strain is synonymous with NCPPB 1811 and belongs to lineage GL 1. Subsequently, genome sequencing revealed that X. albilineans encodes a SPI-1-like T3SS (Pieretti et al., 2009, 2015) and targeted sequencing confirmed its presence in two further Xap GL 1 strains: CFBP 2534 (same as NCPPB 3035) and CFBP 6982 (Marguerettaz et al., 2011). Whole-genome sequencing in the current study indicated that this SPI-1-like T3SS was encoded in the genomes of all GL 1 strains from common bean and Lima bean (Figure 8) but was absent from GL fuscans and from the lablab-associated strains. All of the putative structural genes for the T3SS are conserved in Xap GL 1 but the xapABCDEFGH genes, hypothesized to encode effectors that are substrates of the T3SS in X. albilineans (Marguerettaz et al., 2011), are not conserved in Xap.

Bottom Line: This revealed considerable genetic variation within both taxa, encompassing both single-nucleotide variants and differences in gene content, that could be exploited for tracking pathogen spread.The strains from lablab represent a new, previously unknown genetic lineage closely related to strains of X. axonopodis pv. glycines.Finally, we identified more than 100 genes that appear to have been recently acquired by Xanthomonas axonopodis pv. phaseoli from X. fuscans subsp. fuscans.

View Article: PubMed Central - PubMed

Affiliation: International Center for Tropical Agriculture Kampala, Uganda.

ABSTRACT
Common bacterial blight is a devastating seed-borne disease of common beans that also occurs on other legume species including lablab and Lima beans. We sequenced and analyzed the genomes of 26 strains of Xanthomonas axonopodis pv. phaseoli and X. fuscans subsp. fuscans, the causative agents of this disease, collected over four decades and six continents. This revealed considerable genetic variation within both taxa, encompassing both single-nucleotide variants and differences in gene content, that could be exploited for tracking pathogen spread. The bacterial strain from Lima bean fell within the previously described Genetic Lineage 1, along with the pathovar type strain (NCPPB 3035). The strains from lablab represent a new, previously unknown genetic lineage closely related to strains of X. axonopodis pv. glycines. Finally, we identified more than 100 genes that appear to have been recently acquired by Xanthomonas axonopodis pv. phaseoli from X. fuscans subsp. fuscans.

No MeSH data available.


Related in: MedlinePlus