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Ultra-high-resolution 3D digitalized imaging of the cerebral angioarchitecture in rats using synchrotron radiation.

Zhang MQ, Zhou L, Deng QF, Xie YY, Xiao TQ, Cao YZ, Zhang JW, Chen XM, Yin XZ, Xiao B - Sci Rep (2015)

Bottom Line: This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents.From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum.We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.

ABSTRACT
The angioarchitecture is a fundamental aspect of brain development and physiology. However, available imaging tools are unsuited for non-destructive cerebral mapping of the functionally important three-dimensional (3D) vascular microstructures. To address this issue, we developed an ultra-high resolution 3D digitalized angioarchitectural map for rat brain, based on synchrotron radiation phase contrast imaging (SR-PCI) with pixel size of 5.92 μm. This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents. From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum. We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D. The SR-PCI method for systematic visualization of cerebral microvasculature holds considerable promise for wider application in life sciences, including 3D micro-imaging in experimental models of neurodevelopmental and vascular disorders.

No MeSH data available.


Related in: MedlinePlus

Virtual micro-endoscopy to 3D track targeted vessel.(A) Initiation to orientate a vessel. Successive pathway tracing shown in (B) to (F). The endovascular micro-structure is clearly discernible. Scale bars: 100 μm.
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f8: Virtual micro-endoscopy to 3D track targeted vessel.(A) Initiation to orientate a vessel. Successive pathway tracing shown in (B) to (F). The endovascular micro-structure is clearly discernible. Scale bars: 100 μm.

Mentions: Virtual micro-endoscopy offers a novel perspective for observations within the intravascular space. The 3D micro-structural features of the targeted vascular lumen could be identified (Fig. 8). Concurrent application of 3D virtual flight through the brain space was possible with automatic navigation through the vessel lumen and constitutes a powerful tool for stereoscopic visualization and measurement of endovascular anatomy (Supplementary movie 1).


Ultra-high-resolution 3D digitalized imaging of the cerebral angioarchitecture in rats using synchrotron radiation.

Zhang MQ, Zhou L, Deng QF, Xie YY, Xiao TQ, Cao YZ, Zhang JW, Chen XM, Yin XZ, Xiao B - Sci Rep (2015)

Virtual micro-endoscopy to 3D track targeted vessel.(A) Initiation to orientate a vessel. Successive pathway tracing shown in (B) to (F). The endovascular micro-structure is clearly discernible. Scale bars: 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595735&req=5

f8: Virtual micro-endoscopy to 3D track targeted vessel.(A) Initiation to orientate a vessel. Successive pathway tracing shown in (B) to (F). The endovascular micro-structure is clearly discernible. Scale bars: 100 μm.
Mentions: Virtual micro-endoscopy offers a novel perspective for observations within the intravascular space. The 3D micro-structural features of the targeted vascular lumen could be identified (Fig. 8). Concurrent application of 3D virtual flight through the brain space was possible with automatic navigation through the vessel lumen and constitutes a powerful tool for stereoscopic visualization and measurement of endovascular anatomy (Supplementary movie 1).

Bottom Line: This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents.From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum.We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.

ABSTRACT
The angioarchitecture is a fundamental aspect of brain development and physiology. However, available imaging tools are unsuited for non-destructive cerebral mapping of the functionally important three-dimensional (3D) vascular microstructures. To address this issue, we developed an ultra-high resolution 3D digitalized angioarchitectural map for rat brain, based on synchrotron radiation phase contrast imaging (SR-PCI) with pixel size of 5.92 μm. This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents. From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum. We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D. The SR-PCI method for systematic visualization of cerebral microvasculature holds considerable promise for wider application in life sciences, including 3D micro-imaging in experimental models of neurodevelopmental and vascular disorders.

No MeSH data available.


Related in: MedlinePlus