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Ultra-high-resolution 3D digitalized imaging of the cerebral angioarchitecture in rats using synchrotron radiation.

Zhang MQ, Zhou L, Deng QF, Xie YY, Xiao TQ, Cao YZ, Zhang JW, Chen XM, Yin XZ, Xiao B - Sci Rep (2015)

Bottom Line: From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum.We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D.The SR-PCI method for systematic visualization of cerebral microvasculature holds considerable promise for wider application in life sciences, including 3D micro-imaging in experimental models of neurodevelopmental and vascular disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.

ABSTRACT
The angioarchitecture is a fundamental aspect of brain development and physiology. However, available imaging tools are unsuited for non-destructive cerebral mapping of the functionally important three-dimensional (3D) vascular microstructures. To address this issue, we developed an ultra-high resolution 3D digitalized angioarchitectural map for rat brain, based on synchrotron radiation phase contrast imaging (SR-PCI) with pixel size of 5.92 μm. This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents. From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum. We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D. The SR-PCI method for systematic visualization of cerebral microvasculature holds considerable promise for wider application in life sciences, including 3D micro-imaging in experimental models of neurodevelopmental and vascular disorders.

No MeSH data available.


Related in: MedlinePlus

3D quantitative characterization.(A) Morphometric parameters based on global vascular network analysis. (B) The percentage distribution in quantity of global vessels at different diameter ranges.
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f7: 3D quantitative characterization.(A) Morphometric parameters based on global vascular network analysis. (B) The percentage distribution in quantity of global vessels at different diameter ranges.

Mentions: The most important parameters that emerged as being closely related to the cerebral angioarchitectural profile are listed in Fig. 7A. The fractional frequency distribution of different blood vessel size ranges was also analyzed (Fig. 7B). These results demonstrated that the cerebral vessel branches were extraordinarily numerous, and the abundance of blood vessels significantly increased with decreasing vessel diameter. Approximately 90% of vessels were <30 μm in diameter, and had a distribution correlating highly with the overall structure of the microcirculation, as defined by the scaffold of large vessels.


Ultra-high-resolution 3D digitalized imaging of the cerebral angioarchitecture in rats using synchrotron radiation.

Zhang MQ, Zhou L, Deng QF, Xie YY, Xiao TQ, Cao YZ, Zhang JW, Chen XM, Yin XZ, Xiao B - Sci Rep (2015)

3D quantitative characterization.(A) Morphometric parameters based on global vascular network analysis. (B) The percentage distribution in quantity of global vessels at different diameter ranges.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595735&req=5

f7: 3D quantitative characterization.(A) Morphometric parameters based on global vascular network analysis. (B) The percentage distribution in quantity of global vessels at different diameter ranges.
Mentions: The most important parameters that emerged as being closely related to the cerebral angioarchitectural profile are listed in Fig. 7A. The fractional frequency distribution of different blood vessel size ranges was also analyzed (Fig. 7B). These results demonstrated that the cerebral vessel branches were extraordinarily numerous, and the abundance of blood vessels significantly increased with decreasing vessel diameter. Approximately 90% of vessels were <30 μm in diameter, and had a distribution correlating highly with the overall structure of the microcirculation, as defined by the scaffold of large vessels.

Bottom Line: From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum.We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D.The SR-PCI method for systematic visualization of cerebral microvasculature holds considerable promise for wider application in life sciences, including 3D micro-imaging in experimental models of neurodevelopmental and vascular disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.

ABSTRACT
The angioarchitecture is a fundamental aspect of brain development and physiology. However, available imaging tools are unsuited for non-destructive cerebral mapping of the functionally important three-dimensional (3D) vascular microstructures. To address this issue, we developed an ultra-high resolution 3D digitalized angioarchitectural map for rat brain, based on synchrotron radiation phase contrast imaging (SR-PCI) with pixel size of 5.92 μm. This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents. From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum. We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D. The SR-PCI method for systematic visualization of cerebral microvasculature holds considerable promise for wider application in life sciences, including 3D micro-imaging in experimental models of neurodevelopmental and vascular disorders.

No MeSH data available.


Related in: MedlinePlus