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Ultra-high-resolution 3D digitalized imaging of the cerebral angioarchitecture in rats using synchrotron radiation.

Zhang MQ, Zhou L, Deng QF, Xie YY, Xiao TQ, Cao YZ, Zhang JW, Chen XM, Yin XZ, Xiao B - Sci Rep (2015)

Bottom Line: This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents.From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum.We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.

ABSTRACT
The angioarchitecture is a fundamental aspect of brain development and physiology. However, available imaging tools are unsuited for non-destructive cerebral mapping of the functionally important three-dimensional (3D) vascular microstructures. To address this issue, we developed an ultra-high resolution 3D digitalized angioarchitectural map for rat brain, based on synchrotron radiation phase contrast imaging (SR-PCI) with pixel size of 5.92 μm. This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents. From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum. We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D. The SR-PCI method for systematic visualization of cerebral microvasculature holds considerable promise for wider application in life sciences, including 3D micro-imaging in experimental models of neurodevelopmental and vascular disorders.

No MeSH data available.


Related in: MedlinePlus

Multi-angle surface-rendering of the 3D reconstructed brain.(A) Ventral view. (B) Dorsal view (C) Partial enlargement view of (B) with red box showing the leptomeningeal anastomotic branches. (D) Lateral right view. (E) Lateral left view. (F) The pial branches of middle cerebral artery. Scale bars: 3000 μm (A,B,D,E), 1000 μm (C) and 1500 μm (F).
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f2: Multi-angle surface-rendering of the 3D reconstructed brain.(A) Ventral view. (B) Dorsal view (C) Partial enlargement view of (B) with red box showing the leptomeningeal anastomotic branches. (D) Lateral right view. (E) Lateral left view. (F) The pial branches of middle cerebral artery. Scale bars: 3000 μm (A,B,D,E), 1000 μm (C) and 1500 μm (F).

Mentions: By means of 3D surface volume rendering, the present data authentically outlined vascular trees at the brain surface from multiple perspectives (Fig. 2). Without any use of contrast agent, SR-PCI can thus provide image detail sufficient to clearly show the blood supply at the cortical surface in 3D. The leptomeningeal rami anastomoticus and the multiple pial branches of the middle cerebral artery (MCA) were also well identified, which is propitious for future use of the SR-PCI in studying collateral circulation in diverse biological states.


Ultra-high-resolution 3D digitalized imaging of the cerebral angioarchitecture in rats using synchrotron radiation.

Zhang MQ, Zhou L, Deng QF, Xie YY, Xiao TQ, Cao YZ, Zhang JW, Chen XM, Yin XZ, Xiao B - Sci Rep (2015)

Multi-angle surface-rendering of the 3D reconstructed brain.(A) Ventral view. (B) Dorsal view (C) Partial enlargement view of (B) with red box showing the leptomeningeal anastomotic branches. (D) Lateral right view. (E) Lateral left view. (F) The pial branches of middle cerebral artery. Scale bars: 3000 μm (A,B,D,E), 1000 μm (C) and 1500 μm (F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595735&req=5

f2: Multi-angle surface-rendering of the 3D reconstructed brain.(A) Ventral view. (B) Dorsal view (C) Partial enlargement view of (B) with red box showing the leptomeningeal anastomotic branches. (D) Lateral right view. (E) Lateral left view. (F) The pial branches of middle cerebral artery. Scale bars: 3000 μm (A,B,D,E), 1000 μm (C) and 1500 μm (F).
Mentions: By means of 3D surface volume rendering, the present data authentically outlined vascular trees at the brain surface from multiple perspectives (Fig. 2). Without any use of contrast agent, SR-PCI can thus provide image detail sufficient to clearly show the blood supply at the cortical surface in 3D. The leptomeningeal rami anastomoticus and the multiple pial branches of the middle cerebral artery (MCA) were also well identified, which is propitious for future use of the SR-PCI in studying collateral circulation in diverse biological states.

Bottom Line: This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents.From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum.We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.

ABSTRACT
The angioarchitecture is a fundamental aspect of brain development and physiology. However, available imaging tools are unsuited for non-destructive cerebral mapping of the functionally important three-dimensional (3D) vascular microstructures. To address this issue, we developed an ultra-high resolution 3D digitalized angioarchitectural map for rat brain, based on synchrotron radiation phase contrast imaging (SR-PCI) with pixel size of 5.92 μm. This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents. From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum. We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D. The SR-PCI method for systematic visualization of cerebral microvasculature holds considerable promise for wider application in life sciences, including 3D micro-imaging in experimental models of neurodevelopmental and vascular disorders.

No MeSH data available.


Related in: MedlinePlus