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Intracerebroventricular Injection of Alarin Increased Glucose Uptake in Skeletal Muscle of Diabetic Rats.

Zhang Z, Wu Y, Sheng S, Guo L, He B, Fang P, Shi M, Bo P, Zhu Y - PLoS ONE (2015)

Bottom Line: We found that central treatment with alarin significantly increased the food intake, body weight and glucose infusion rates in hyperinsulinemic euglycemic clamp tests of the animals.Besides, the treatment also enhanced 2-deoxy-[3H]-D-glucose uptake, vesicle-associated membrane protein 2 contents, glucose transporter 4 protein and mRNA expression, as well as pAktThr308, pAktSer473 and total Akt levels in muscle cells, but reduced plasma glucose and insulin levels of the rats.These results suggest that central administration of alarin stimulates glucose uptake mediated by activation of Akt signal pathway in type 2 diabetic animals.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu, China, 225001.

ABSTRACT
In order to investigate the central effect of alarin on glucose uptake, we administered alarin and/ or its inhibitor, ala6-25Cys into the cerebral ventricles of the type 2 diabetic rats. Then the relative parameters about glucose uptake in skeletal muscles were measured. We found that central treatment with alarin significantly increased the food intake, body weight and glucose infusion rates in hyperinsulinemic euglycemic clamp tests of the animals. Besides, the treatment also enhanced 2-deoxy-[3H]-D-glucose uptake, vesicle-associated membrane protein 2 contents, glucose transporter 4 protein and mRNA expression, as well as pAktThr308, pAktSer473 and total Akt levels in muscle cells, but reduced plasma glucose and insulin levels of the rats. All of the alarin-inducing events may be antagonised by central injection of ala6-25Cys. These results suggest that central administration of alarin stimulates glucose uptake mediated by activation of Akt signal pathway in type 2 diabetic animals.

No MeSH data available.


Related in: MedlinePlus

Central alarin administration increased pAktThr308, pAktSer473 and total Akt levels in the skeletal muscles of the rats (n = 8).The AktThr308,and AktSer473 phosphorylation as well as total Akt level were higher in the alarin group than each diabetic control (DC). Three levels in the ala6-25Cys+alarin group (ala6-25Cys+Al) were higher than the ala6-25Cys group, but lower than the alarin group. Three parameters in the diabetic control group were lower than health controls (HC). 1 presents the health control group, 2 the diabetic control group, 3 the ala6-25Cys group, 4 the alarin group, 5 the ala6-25Cys+alarin group. The data shown are the means ± SEM. ●● P < 0.01 vs. HC; * P < 0.05, ** P < 0.01 vs. DC; # P < 0.05 vs ala6-25Cys group; △P < 0.05, △△ P < 0.01 vs. alarin group.
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pone.0139327.g008: Central alarin administration increased pAktThr308, pAktSer473 and total Akt levels in the skeletal muscles of the rats (n = 8).The AktThr308,and AktSer473 phosphorylation as well as total Akt level were higher in the alarin group than each diabetic control (DC). Three levels in the ala6-25Cys+alarin group (ala6-25Cys+Al) were higher than the ala6-25Cys group, but lower than the alarin group. Three parameters in the diabetic control group were lower than health controls (HC). 1 presents the health control group, 2 the diabetic control group, 3 the ala6-25Cys group, 4 the alarin group, 5 the ala6-25Cys+alarin group. The data shown are the means ± SEM. ●● P < 0.01 vs. HC; * P < 0.05, ** P < 0.01 vs. DC; # P < 0.05 vs ala6-25Cys group; △P < 0.05, △△ P < 0.01 vs. alarin group.

Mentions: In order to determine the signaling pathway of alarin, we measured pAktThr308, pAktSer473 and total Akt levels in the muscles [11]. As shown in Fig 8, central injection of alarin significantly elevated pAktThr308, pAktSer473 and total Akt levels by 47.6% (P < 0.01), 23.6% (P < 0.05) and 12.3% (P < 0.05) compared with each diabetic control in the skeletal muscles. The three indexes in the ala6-25Cys + alarin group were respectively attenuated by 33.5% (P < 0.01), 16.4% (P < 0.05) and 10.1% (P < 0.05) compared with the alarin group, but enhanced by 21.2% (P < 0.05), 30.6% (P < 0.01) and 26.2% (P < 0.01) compared with the ala6-25Cys group. As well, the three parameters in the diabetic control group were lower than healthy controls (P < 0.01) (S8 File).


Intracerebroventricular Injection of Alarin Increased Glucose Uptake in Skeletal Muscle of Diabetic Rats.

Zhang Z, Wu Y, Sheng S, Guo L, He B, Fang P, Shi M, Bo P, Zhu Y - PLoS ONE (2015)

Central alarin administration increased pAktThr308, pAktSer473 and total Akt levels in the skeletal muscles of the rats (n = 8).The AktThr308,and AktSer473 phosphorylation as well as total Akt level were higher in the alarin group than each diabetic control (DC). Three levels in the ala6-25Cys+alarin group (ala6-25Cys+Al) were higher than the ala6-25Cys group, but lower than the alarin group. Three parameters in the diabetic control group were lower than health controls (HC). 1 presents the health control group, 2 the diabetic control group, 3 the ala6-25Cys group, 4 the alarin group, 5 the ala6-25Cys+alarin group. The data shown are the means ± SEM. ●● P < 0.01 vs. HC; * P < 0.05, ** P < 0.01 vs. DC; # P < 0.05 vs ala6-25Cys group; △P < 0.05, △△ P < 0.01 vs. alarin group.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4595443&req=5

pone.0139327.g008: Central alarin administration increased pAktThr308, pAktSer473 and total Akt levels in the skeletal muscles of the rats (n = 8).The AktThr308,and AktSer473 phosphorylation as well as total Akt level were higher in the alarin group than each diabetic control (DC). Three levels in the ala6-25Cys+alarin group (ala6-25Cys+Al) were higher than the ala6-25Cys group, but lower than the alarin group. Three parameters in the diabetic control group were lower than health controls (HC). 1 presents the health control group, 2 the diabetic control group, 3 the ala6-25Cys group, 4 the alarin group, 5 the ala6-25Cys+alarin group. The data shown are the means ± SEM. ●● P < 0.01 vs. HC; * P < 0.05, ** P < 0.01 vs. DC; # P < 0.05 vs ala6-25Cys group; △P < 0.05, △△ P < 0.01 vs. alarin group.
Mentions: In order to determine the signaling pathway of alarin, we measured pAktThr308, pAktSer473 and total Akt levels in the muscles [11]. As shown in Fig 8, central injection of alarin significantly elevated pAktThr308, pAktSer473 and total Akt levels by 47.6% (P < 0.01), 23.6% (P < 0.05) and 12.3% (P < 0.05) compared with each diabetic control in the skeletal muscles. The three indexes in the ala6-25Cys + alarin group were respectively attenuated by 33.5% (P < 0.01), 16.4% (P < 0.05) and 10.1% (P < 0.05) compared with the alarin group, but enhanced by 21.2% (P < 0.05), 30.6% (P < 0.01) and 26.2% (P < 0.01) compared with the ala6-25Cys group. As well, the three parameters in the diabetic control group were lower than healthy controls (P < 0.01) (S8 File).

Bottom Line: We found that central treatment with alarin significantly increased the food intake, body weight and glucose infusion rates in hyperinsulinemic euglycemic clamp tests of the animals.Besides, the treatment also enhanced 2-deoxy-[3H]-D-glucose uptake, vesicle-associated membrane protein 2 contents, glucose transporter 4 protein and mRNA expression, as well as pAktThr308, pAktSer473 and total Akt levels in muscle cells, but reduced plasma glucose and insulin levels of the rats.These results suggest that central administration of alarin stimulates glucose uptake mediated by activation of Akt signal pathway in type 2 diabetic animals.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu, China, 225001.

ABSTRACT
In order to investigate the central effect of alarin on glucose uptake, we administered alarin and/ or its inhibitor, ala6-25Cys into the cerebral ventricles of the type 2 diabetic rats. Then the relative parameters about glucose uptake in skeletal muscles were measured. We found that central treatment with alarin significantly increased the food intake, body weight and glucose infusion rates in hyperinsulinemic euglycemic clamp tests of the animals. Besides, the treatment also enhanced 2-deoxy-[3H]-D-glucose uptake, vesicle-associated membrane protein 2 contents, glucose transporter 4 protein and mRNA expression, as well as pAktThr308, pAktSer473 and total Akt levels in muscle cells, but reduced plasma glucose and insulin levels of the rats. All of the alarin-inducing events may be antagonised by central injection of ala6-25Cys. These results suggest that central administration of alarin stimulates glucose uptake mediated by activation of Akt signal pathway in type 2 diabetic animals.

No MeSH data available.


Related in: MedlinePlus