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Intracerebroventricular Injection of Alarin Increased Glucose Uptake in Skeletal Muscle of Diabetic Rats.

Zhang Z, Wu Y, Sheng S, Guo L, He B, Fang P, Shi M, Bo P, Zhu Y - PLoS ONE (2015)

Bottom Line: We found that central treatment with alarin significantly increased the food intake, body weight and glucose infusion rates in hyperinsulinemic euglycemic clamp tests of the animals.Besides, the treatment also enhanced 2-deoxy-[3H]-D-glucose uptake, vesicle-associated membrane protein 2 contents, glucose transporter 4 protein and mRNA expression, as well as pAktThr308, pAktSer473 and total Akt levels in muscle cells, but reduced plasma glucose and insulin levels of the rats.These results suggest that central administration of alarin stimulates glucose uptake mediated by activation of Akt signal pathway in type 2 diabetic animals.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu, China, 225001.

ABSTRACT
In order to investigate the central effect of alarin on glucose uptake, we administered alarin and/ or its inhibitor, ala6-25Cys into the cerebral ventricles of the type 2 diabetic rats. Then the relative parameters about glucose uptake in skeletal muscles were measured. We found that central treatment with alarin significantly increased the food intake, body weight and glucose infusion rates in hyperinsulinemic euglycemic clamp tests of the animals. Besides, the treatment also enhanced 2-deoxy-[3H]-D-glucose uptake, vesicle-associated membrane protein 2 contents, glucose transporter 4 protein and mRNA expression, as well as pAktThr308, pAktSer473 and total Akt levels in muscle cells, but reduced plasma glucose and insulin levels of the rats. All of the alarin-inducing events may be antagonised by central injection of ala6-25Cys. These results suggest that central administration of alarin stimulates glucose uptake mediated by activation of Akt signal pathway in type 2 diabetic animals.

No MeSH data available.


Related in: MedlinePlus

The central alarin administration significantly elevated glucose infusing rates in hyperinsulinemic-euglycemic clamp tests in the rats (n = 8).The infusing rates were higher in the alarin group than diabetic controls (DC). In the ala6-25Cys+alarin group (ala6-25Cys+Al) the rates were increased compared with the ala6-25Cys group, but attenuated compared with the alarin group. In addition, the rate in the diabetic control group was lower than healthy controls (HC). The data shown are the means ± SEM. ●● P < 0.01 vs. HC; * P < 0.05 vs. DC; # P < 0.05 vs ala6-25Cys group; △P < 0.05 vs. alarin group.
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pone.0139327.g004: The central alarin administration significantly elevated glucose infusing rates in hyperinsulinemic-euglycemic clamp tests in the rats (n = 8).The infusing rates were higher in the alarin group than diabetic controls (DC). In the ala6-25Cys+alarin group (ala6-25Cys+Al) the rates were increased compared with the ala6-25Cys group, but attenuated compared with the alarin group. In addition, the rate in the diabetic control group was lower than healthy controls (HC). The data shown are the means ± SEM. ●● P < 0.01 vs. HC; * P < 0.05 vs. DC; # P < 0.05 vs ala6-25Cys group; △P < 0.05 vs. alarin group.

Mentions: The central administration of alarin significantly elevated glucose infusing rates in the hyperinsulinemic-euglycemic clamp tests. As compared with diabetic controls, the infusion rates in the alarin group increased by 25.4% (P < 0.05), which were attenuated by co-treatment with ala6-25Cys (Fig 4). In the ala6-25Cys+alarin group the rats were enhanced by 33.3% (P < 0.05) compared with the ala6-25Cys group, but decreased by 18.5% (P < 0.05) compared with the alarin group. As same, the infusion rates were significantly lower (P < 0.01) in the diabetic control group than healthy controls (S5 File).


Intracerebroventricular Injection of Alarin Increased Glucose Uptake in Skeletal Muscle of Diabetic Rats.

Zhang Z, Wu Y, Sheng S, Guo L, He B, Fang P, Shi M, Bo P, Zhu Y - PLoS ONE (2015)

The central alarin administration significantly elevated glucose infusing rates in hyperinsulinemic-euglycemic clamp tests in the rats (n = 8).The infusing rates were higher in the alarin group than diabetic controls (DC). In the ala6-25Cys+alarin group (ala6-25Cys+Al) the rates were increased compared with the ala6-25Cys group, but attenuated compared with the alarin group. In addition, the rate in the diabetic control group was lower than healthy controls (HC). The data shown are the means ± SEM. ●● P < 0.01 vs. HC; * P < 0.05 vs. DC; # P < 0.05 vs ala6-25Cys group; △P < 0.05 vs. alarin group.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4595443&req=5

pone.0139327.g004: The central alarin administration significantly elevated glucose infusing rates in hyperinsulinemic-euglycemic clamp tests in the rats (n = 8).The infusing rates were higher in the alarin group than diabetic controls (DC). In the ala6-25Cys+alarin group (ala6-25Cys+Al) the rates were increased compared with the ala6-25Cys group, but attenuated compared with the alarin group. In addition, the rate in the diabetic control group was lower than healthy controls (HC). The data shown are the means ± SEM. ●● P < 0.01 vs. HC; * P < 0.05 vs. DC; # P < 0.05 vs ala6-25Cys group; △P < 0.05 vs. alarin group.
Mentions: The central administration of alarin significantly elevated glucose infusing rates in the hyperinsulinemic-euglycemic clamp tests. As compared with diabetic controls, the infusion rates in the alarin group increased by 25.4% (P < 0.05), which were attenuated by co-treatment with ala6-25Cys (Fig 4). In the ala6-25Cys+alarin group the rats were enhanced by 33.3% (P < 0.05) compared with the ala6-25Cys group, but decreased by 18.5% (P < 0.05) compared with the alarin group. As same, the infusion rates were significantly lower (P < 0.01) in the diabetic control group than healthy controls (S5 File).

Bottom Line: We found that central treatment with alarin significantly increased the food intake, body weight and glucose infusion rates in hyperinsulinemic euglycemic clamp tests of the animals.Besides, the treatment also enhanced 2-deoxy-[3H]-D-glucose uptake, vesicle-associated membrane protein 2 contents, glucose transporter 4 protein and mRNA expression, as well as pAktThr308, pAktSer473 and total Akt levels in muscle cells, but reduced plasma glucose and insulin levels of the rats.These results suggest that central administration of alarin stimulates glucose uptake mediated by activation of Akt signal pathway in type 2 diabetic animals.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu, China, 225001.

ABSTRACT
In order to investigate the central effect of alarin on glucose uptake, we administered alarin and/ or its inhibitor, ala6-25Cys into the cerebral ventricles of the type 2 diabetic rats. Then the relative parameters about glucose uptake in skeletal muscles were measured. We found that central treatment with alarin significantly increased the food intake, body weight and glucose infusion rates in hyperinsulinemic euglycemic clamp tests of the animals. Besides, the treatment also enhanced 2-deoxy-[3H]-D-glucose uptake, vesicle-associated membrane protein 2 contents, glucose transporter 4 protein and mRNA expression, as well as pAktThr308, pAktSer473 and total Akt levels in muscle cells, but reduced plasma glucose and insulin levels of the rats. All of the alarin-inducing events may be antagonised by central injection of ala6-25Cys. These results suggest that central administration of alarin stimulates glucose uptake mediated by activation of Akt signal pathway in type 2 diabetic animals.

No MeSH data available.


Related in: MedlinePlus