Limits...
Subretinal heterotopic respiratory epithelium: a case report and literature review.

Zhang T, Lin H, Zhou Z, Tong N, Li Y, Zhang Y, Wang L, Liu F - Diagn Pathol (2015)

Bottom Line: A 51-year-old female underwent vitrectomy surgery to remove a group of spherical subretinal tumors beneath the detached retina.Hematoxylin and eosin staining and immunohistochemical findings showed that the characteristics of the tumor were consistent with a subretinal heterotopic respiratory epithelium.This is the first report of a respiratory epithelial heterotopia located in the subretinal space.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Qingdao Municipal Hospital, No.1 Jiaozhou Road, Shibei District, Qingdao, Shandong, China.

ABSTRACT
A 51-year-old female underwent vitrectomy surgery to remove a group of spherical subretinal tumors beneath the detached retina. Hematoxylin and eosin staining and immunohistochemical findings showed that the characteristics of the tumor were consistent with a subretinal heterotopic respiratory epithelium. This is the first report of a respiratory epithelial heterotopia located in the subretinal space.

No MeSH data available.


Related in: MedlinePlus

IHC staining for CK, GFAP and S100. The strong positive staining of CK is shown in (a). While negative staining images of GFAP and S100 are shown in (b) and (c), respectively
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4595248&req=5

Fig4: IHC staining for CK, GFAP and S100. The strong positive staining of CK is shown in (a). While negative staining images of GFAP and S100 are shown in (b) and (c), respectively

Mentions: The excised mass was fixed in 10 % formalin and embedded in paraffin. Several 6-μm sections were cut from each paraffin block, two sections were stained with hematoxylin and eosin (H&E) and periodic acid Schiff-Alcian blue, and the others were stained with various antibodies (immunohistochemistry (IHC)). Immunohistochemical staining was performed using the streptavidin-peroxidase system (Ultrasensitive; Mai Xin Inc., Fuzhou, China) according to the manufacturer’s instructions. Commercially available, pre-diluted monoclonal antibodies against the following antigens were used: cytokeratin (CK), nerve tissue protein S100, glial fibrillary acidic protein (GFAP) (all Thermo Fisher Scientific Inc., Fremont, CA, USA). H&E staining revealed that the tumor was solid and covered with pseudostratified ciliated columnar epithelium containing scattered goblet cells, which were prominently shown by Alcian blue stain (representative goblet cell was shown in Fig. 3a and b by arrow). There was loose connective tissue just beneath the epithelia, which contained blood vessels, fibroblasts, fibrocytes, lymphocytes, plasmocytes, and macrophages. IHC was positive for CK (Fig. 4a), but negative for S100 (Fig. 4b) and GFAP (Fig. 4c).Fig. 3


Subretinal heterotopic respiratory epithelium: a case report and literature review.

Zhang T, Lin H, Zhou Z, Tong N, Li Y, Zhang Y, Wang L, Liu F - Diagn Pathol (2015)

IHC staining for CK, GFAP and S100. The strong positive staining of CK is shown in (a). While negative staining images of GFAP and S100 are shown in (b) and (c), respectively
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4595248&req=5

Fig4: IHC staining for CK, GFAP and S100. The strong positive staining of CK is shown in (a). While negative staining images of GFAP and S100 are shown in (b) and (c), respectively
Mentions: The excised mass was fixed in 10 % formalin and embedded in paraffin. Several 6-μm sections were cut from each paraffin block, two sections were stained with hematoxylin and eosin (H&E) and periodic acid Schiff-Alcian blue, and the others were stained with various antibodies (immunohistochemistry (IHC)). Immunohistochemical staining was performed using the streptavidin-peroxidase system (Ultrasensitive; Mai Xin Inc., Fuzhou, China) according to the manufacturer’s instructions. Commercially available, pre-diluted monoclonal antibodies against the following antigens were used: cytokeratin (CK), nerve tissue protein S100, glial fibrillary acidic protein (GFAP) (all Thermo Fisher Scientific Inc., Fremont, CA, USA). H&E staining revealed that the tumor was solid and covered with pseudostratified ciliated columnar epithelium containing scattered goblet cells, which were prominently shown by Alcian blue stain (representative goblet cell was shown in Fig. 3a and b by arrow). There was loose connective tissue just beneath the epithelia, which contained blood vessels, fibroblasts, fibrocytes, lymphocytes, plasmocytes, and macrophages. IHC was positive for CK (Fig. 4a), but negative for S100 (Fig. 4b) and GFAP (Fig. 4c).Fig. 3

Bottom Line: A 51-year-old female underwent vitrectomy surgery to remove a group of spherical subretinal tumors beneath the detached retina.Hematoxylin and eosin staining and immunohistochemical findings showed that the characteristics of the tumor were consistent with a subretinal heterotopic respiratory epithelium.This is the first report of a respiratory epithelial heterotopia located in the subretinal space.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Qingdao Municipal Hospital, No.1 Jiaozhou Road, Shibei District, Qingdao, Shandong, China.

ABSTRACT
A 51-year-old female underwent vitrectomy surgery to remove a group of spherical subretinal tumors beneath the detached retina. Hematoxylin and eosin staining and immunohistochemical findings showed that the characteristics of the tumor were consistent with a subretinal heterotopic respiratory epithelium. This is the first report of a respiratory epithelial heterotopia located in the subretinal space.

No MeSH data available.


Related in: MedlinePlus