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Wheat WCBP1 encodes a putative copper-binding protein involved in stripe rust resistance and inhibition of leaf senescence.

Li X, Liu T, Chen W, Zhong S, Zhang H, Tang Z, Chang Z, Wang L, Zhang M, Li L, Rao H, Ren Z, Luo P - BMC Plant Biol. (2015)

Bottom Line: We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval.Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. sadoneli@gmail.com.

ABSTRACT

Background: Stripe rust, a highly destructive foliar disease of wheat (Triticum aestivum), causes severe losses, which may be accompanied by reduced photosynthetic activity and accelerated leaf senescence.

Methods: We used suppression subtractive hybridization (SSH) to examine the mechanisms of resistance in the resistant wheat line L693 (Reg. No. GP-972, PI 672538), which was derived from a lineage that includes a wide cross between common and Thinopyrum intermedium. Sequencing of an SSH cDNA library identified 112 expressed sequence tags.

Results: In silico mapping placed one of these tags [GenBank: JK972238] on chromosome 1A. Primers based on [GenBank: JK972238] amplified a polymorphic band, which co-segregated with YrL693. We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval. Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.

Discussion: Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.

Conclusions: The unique chromosomal location and expression mode of WCBP1 suggested that WCBP1 is the putative candidate gene of YrL693, which was involved in leaf senescence and photosynthesis related to plant responses to stripe rust infection during the grain-filling stage.

No MeSH data available.


Related in: MedlinePlus

Chlorophyll fluorescence-related parameters used to determine differences in photosystem II (PSII) activity between the resistant line L693 and the susceptible line L661 following Pst inoculation and after heading. a, Values for the maximal photochemical efficiency of PSII (Fv/Fm); b, the efficiency of excitation capture by the open PSII reaction centers (F’v/F’m); c, the photochemical quenching coefficient (qP); d, quantum yield of photochemical energy conversion in PSII (ΦPSII); e, quantum yield of regulated non-photochemical energy loss in PSII (NPQ); and f, quantum yield of non-regulated non-photochemical energy loss in PSII (NO) in wheat flag leaves. The parameters ΦPSII, NPQ and NO were derived from averages of related parameters according to a previously described method [36]. The disease had fully developed at heading, and 10 plants per genotype were used for the measurements. Bars represent the standard error, and significance was determined using independent sample t-tests. Asterisks represent significant differences as follows: **P ≤0.01, *P ≤0.05, and Ns for no significant difference. Raised asterisks or NS represent the differences in gene expression between L693 and L661 at each time point. The asterisks or the Ns label on the trend line represent differences in gene expression between two adjacent time points in the same genotype
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Fig8: Chlorophyll fluorescence-related parameters used to determine differences in photosystem II (PSII) activity between the resistant line L693 and the susceptible line L661 following Pst inoculation and after heading. a, Values for the maximal photochemical efficiency of PSII (Fv/Fm); b, the efficiency of excitation capture by the open PSII reaction centers (F’v/F’m); c, the photochemical quenching coefficient (qP); d, quantum yield of photochemical energy conversion in PSII (ΦPSII); e, quantum yield of regulated non-photochemical energy loss in PSII (NPQ); and f, quantum yield of non-regulated non-photochemical energy loss in PSII (NO) in wheat flag leaves. The parameters ΦPSII, NPQ and NO were derived from averages of related parameters according to a previously described method [36]. The disease had fully developed at heading, and 10 plants per genotype were used for the measurements. Bars represent the standard error, and significance was determined using independent sample t-tests. Asterisks represent significant differences as follows: **P ≤0.01, *P ≤0.05, and Ns for no significant difference. Raised asterisks or NS represent the differences in gene expression between L693 and L661 at each time point. The asterisks or the Ns label on the trend line represent differences in gene expression between two adjacent time points in the same genotype

Mentions: Although the changes in the maximal photochemical efficiency of PSІІ in the dark-adapted leaves (Fv/Fm) were similar between L693 and L661, the Fv/Fm value obtained for L693 was significantly higher than that obtained for L661 at 30 days after heading. A significant decrease in Fv/Fm occurred in L661 between 20 days and 30 days after heading, whereas only a slight decrease occurred in this timeframe in L693 (Fig. 8a). The efficiencies of excitation capture by the open PSII reaction centers (Fv‘/ Fm’) observed in L693 were significantly lower than, similar to, and higher than those recorded in L693 prior to 20 days, at 20 days and at 30 days after heading, respectively (Fig. 8b). The photochemical quenching coefficient (qP) was similar in L693 and L661. Although the qP values obtained in L693 were greater than those in L661, the differences were not significant at the P = 0.05 level (Fig. 8c). The changes in the quantum yield of photochemical energy conversion in PSII (ΦPSII) (Fig. 8d), the quantum yield of regulated non-photochemical energy loss in PSII (NPQ) (Fig. 8e), and the quantum yield of non-regulated, non-photochemical energy loss in PSII (NO) (Fig. 8f) were quite similar in L693 and L661. The value of ΦPSII recorded in L693 was significantly lower at the heading stage and significantly greater at 30 days after heading, compared with that in L661 (Fig. 8d). In L693, the value of NPQ obtained was significantly greater than that in L661 at the heading stage (Fig. 8e), and the value of NO was significantly lower than that in L661 at 30 days after heading (Fig. 8f).Fig. 8


Wheat WCBP1 encodes a putative copper-binding protein involved in stripe rust resistance and inhibition of leaf senescence.

Li X, Liu T, Chen W, Zhong S, Zhang H, Tang Z, Chang Z, Wang L, Zhang M, Li L, Rao H, Ren Z, Luo P - BMC Plant Biol. (2015)

Chlorophyll fluorescence-related parameters used to determine differences in photosystem II (PSII) activity between the resistant line L693 and the susceptible line L661 following Pst inoculation and after heading. a, Values for the maximal photochemical efficiency of PSII (Fv/Fm); b, the efficiency of excitation capture by the open PSII reaction centers (F’v/F’m); c, the photochemical quenching coefficient (qP); d, quantum yield of photochemical energy conversion in PSII (ΦPSII); e, quantum yield of regulated non-photochemical energy loss in PSII (NPQ); and f, quantum yield of non-regulated non-photochemical energy loss in PSII (NO) in wheat flag leaves. The parameters ΦPSII, NPQ and NO were derived from averages of related parameters according to a previously described method [36]. The disease had fully developed at heading, and 10 plants per genotype were used for the measurements. Bars represent the standard error, and significance was determined using independent sample t-tests. Asterisks represent significant differences as follows: **P ≤0.01, *P ≤0.05, and Ns for no significant difference. Raised asterisks or NS represent the differences in gene expression between L693 and L661 at each time point. The asterisks or the Ns label on the trend line represent differences in gene expression between two adjacent time points in the same genotype
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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Fig8: Chlorophyll fluorescence-related parameters used to determine differences in photosystem II (PSII) activity between the resistant line L693 and the susceptible line L661 following Pst inoculation and after heading. a, Values for the maximal photochemical efficiency of PSII (Fv/Fm); b, the efficiency of excitation capture by the open PSII reaction centers (F’v/F’m); c, the photochemical quenching coefficient (qP); d, quantum yield of photochemical energy conversion in PSII (ΦPSII); e, quantum yield of regulated non-photochemical energy loss in PSII (NPQ); and f, quantum yield of non-regulated non-photochemical energy loss in PSII (NO) in wheat flag leaves. The parameters ΦPSII, NPQ and NO were derived from averages of related parameters according to a previously described method [36]. The disease had fully developed at heading, and 10 plants per genotype were used for the measurements. Bars represent the standard error, and significance was determined using independent sample t-tests. Asterisks represent significant differences as follows: **P ≤0.01, *P ≤0.05, and Ns for no significant difference. Raised asterisks or NS represent the differences in gene expression between L693 and L661 at each time point. The asterisks or the Ns label on the trend line represent differences in gene expression between two adjacent time points in the same genotype
Mentions: Although the changes in the maximal photochemical efficiency of PSІІ in the dark-adapted leaves (Fv/Fm) were similar between L693 and L661, the Fv/Fm value obtained for L693 was significantly higher than that obtained for L661 at 30 days after heading. A significant decrease in Fv/Fm occurred in L661 between 20 days and 30 days after heading, whereas only a slight decrease occurred in this timeframe in L693 (Fig. 8a). The efficiencies of excitation capture by the open PSII reaction centers (Fv‘/ Fm’) observed in L693 were significantly lower than, similar to, and higher than those recorded in L693 prior to 20 days, at 20 days and at 30 days after heading, respectively (Fig. 8b). The photochemical quenching coefficient (qP) was similar in L693 and L661. Although the qP values obtained in L693 were greater than those in L661, the differences were not significant at the P = 0.05 level (Fig. 8c). The changes in the quantum yield of photochemical energy conversion in PSII (ΦPSII) (Fig. 8d), the quantum yield of regulated non-photochemical energy loss in PSII (NPQ) (Fig. 8e), and the quantum yield of non-regulated, non-photochemical energy loss in PSII (NO) (Fig. 8f) were quite similar in L693 and L661. The value of ΦPSII recorded in L693 was significantly lower at the heading stage and significantly greater at 30 days after heading, compared with that in L661 (Fig. 8d). In L693, the value of NPQ obtained was significantly greater than that in L661 at the heading stage (Fig. 8e), and the value of NO was significantly lower than that in L661 at 30 days after heading (Fig. 8f).Fig. 8

Bottom Line: We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval.Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. sadoneli@gmail.com.

ABSTRACT

Background: Stripe rust, a highly destructive foliar disease of wheat (Triticum aestivum), causes severe losses, which may be accompanied by reduced photosynthetic activity and accelerated leaf senescence.

Methods: We used suppression subtractive hybridization (SSH) to examine the mechanisms of resistance in the resistant wheat line L693 (Reg. No. GP-972, PI 672538), which was derived from a lineage that includes a wide cross between common and Thinopyrum intermedium. Sequencing of an SSH cDNA library identified 112 expressed sequence tags.

Results: In silico mapping placed one of these tags [GenBank: JK972238] on chromosome 1A. Primers based on [GenBank: JK972238] amplified a polymorphic band, which co-segregated with YrL693. We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval. Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.

Discussion: Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.

Conclusions: The unique chromosomal location and expression mode of WCBP1 suggested that WCBP1 is the putative candidate gene of YrL693, which was involved in leaf senescence and photosynthesis related to plant responses to stripe rust infection during the grain-filling stage.

No MeSH data available.


Related in: MedlinePlus