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Wheat WCBP1 encodes a putative copper-binding protein involved in stripe rust resistance and inhibition of leaf senescence.

Li X, Liu T, Chen W, Zhong S, Zhang H, Tang Z, Chang Z, Wang L, Zhang M, Li L, Rao H, Ren Z, Luo P - BMC Plant Biol. (2015)

Bottom Line: We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval.Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. sadoneli@gmail.com.

ABSTRACT

Background: Stripe rust, a highly destructive foliar disease of wheat (Triticum aestivum), causes severe losses, which may be accompanied by reduced photosynthetic activity and accelerated leaf senescence.

Methods: We used suppression subtractive hybridization (SSH) to examine the mechanisms of resistance in the resistant wheat line L693 (Reg. No. GP-972, PI 672538), which was derived from a lineage that includes a wide cross between common and Thinopyrum intermedium. Sequencing of an SSH cDNA library identified 112 expressed sequence tags.

Results: In silico mapping placed one of these tags [GenBank: JK972238] on chromosome 1A. Primers based on [GenBank: JK972238] amplified a polymorphic band, which co-segregated with YrL693. We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval. Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.

Discussion: Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.

Conclusions: The unique chromosomal location and expression mode of WCBP1 suggested that WCBP1 is the putative candidate gene of YrL693, which was involved in leaf senescence and photosynthesis related to plant responses to stripe rust infection during the grain-filling stage.

No MeSH data available.


Related in: MedlinePlus

Comparative genomic mapping of ESTs in the WCBP1 (red) chromosomal region of wheat chromosome 1B (purple) with Brachypodium distachyon chromosome 3 (green), Oryza sativa japonica chromosome 10 (orange), and Sorghum bicolor chromosome 1 (red). The gene order in all species is clockwise
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Fig4: Comparative genomic mapping of ESTs in the WCBP1 (red) chromosomal region of wheat chromosome 1B (purple) with Brachypodium distachyon chromosome 3 (green), Oryza sativa japonica chromosome 10 (orange), and Sorghum bicolor chromosome 1 (red). The gene order in all species is clockwise

Mentions: Orthologs of wheat ESTs BE518403 and BE444094 were detected on B. distachyon chromosome 3, rice chromosome 10, and sorghum chromosome 1. Comparative genomic analysis established the collinearity of WCBP1 genomic regions with a 2.22 Mb (Bradi3g26160–Bradi3g27877) region on B. distachyon chromosome 3, a 3.49 Mb region (Os10g0396400–Os10g0456500) on rice chromosome 10, and a 8.12 Mb region (Sbo1g020230–Sb01g023110) on sorghum chromosome 1, regions that carry 112, 260, and 297 genes, respectively. The gene order was highly conserved among wheat, B. distachyon, and rice (Fig. 4), but showed an intra-chromosomal inversion in the 8.12 Mb chromosome region of sorghum. We also found an orthologous genomic region (from 27,059,150 to 27,060,251) of wheat contig 1BS-3450437 carrying the SSR marker Xgwm18-1B at 27.06 Mb on B. distachyon chromosome 3 (E-value = 5.34E−34), but there was no corresponding ortholog in either rice or sorghum (Fig. 3). An ortholog of WCBP1 was found on Bradi3g27550 (E-value ≈ 0) at 28 Mb on B. distachyon chromosome 3, on Os10g0440500 (E-value = 5.34E−168) at 16 Mb on rice chromosome 10, and on Sb01g020970 (E-value = 1.14E−130) at 24.05 Mb on sorghum chromosome 1 (Figs. 3 and 4).Fig. 4


Wheat WCBP1 encodes a putative copper-binding protein involved in stripe rust resistance and inhibition of leaf senescence.

Li X, Liu T, Chen W, Zhong S, Zhang H, Tang Z, Chang Z, Wang L, Zhang M, Li L, Rao H, Ren Z, Luo P - BMC Plant Biol. (2015)

Comparative genomic mapping of ESTs in the WCBP1 (red) chromosomal region of wheat chromosome 1B (purple) with Brachypodium distachyon chromosome 3 (green), Oryza sativa japonica chromosome 10 (orange), and Sorghum bicolor chromosome 1 (red). The gene order in all species is clockwise
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4595213&req=5

Fig4: Comparative genomic mapping of ESTs in the WCBP1 (red) chromosomal region of wheat chromosome 1B (purple) with Brachypodium distachyon chromosome 3 (green), Oryza sativa japonica chromosome 10 (orange), and Sorghum bicolor chromosome 1 (red). The gene order in all species is clockwise
Mentions: Orthologs of wheat ESTs BE518403 and BE444094 were detected on B. distachyon chromosome 3, rice chromosome 10, and sorghum chromosome 1. Comparative genomic analysis established the collinearity of WCBP1 genomic regions with a 2.22 Mb (Bradi3g26160–Bradi3g27877) region on B. distachyon chromosome 3, a 3.49 Mb region (Os10g0396400–Os10g0456500) on rice chromosome 10, and a 8.12 Mb region (Sbo1g020230–Sb01g023110) on sorghum chromosome 1, regions that carry 112, 260, and 297 genes, respectively. The gene order was highly conserved among wheat, B. distachyon, and rice (Fig. 4), but showed an intra-chromosomal inversion in the 8.12 Mb chromosome region of sorghum. We also found an orthologous genomic region (from 27,059,150 to 27,060,251) of wheat contig 1BS-3450437 carrying the SSR marker Xgwm18-1B at 27.06 Mb on B. distachyon chromosome 3 (E-value = 5.34E−34), but there was no corresponding ortholog in either rice or sorghum (Fig. 3). An ortholog of WCBP1 was found on Bradi3g27550 (E-value ≈ 0) at 28 Mb on B. distachyon chromosome 3, on Os10g0440500 (E-value = 5.34E−168) at 16 Mb on rice chromosome 10, and on Sb01g020970 (E-value = 1.14E−130) at 24.05 Mb on sorghum chromosome 1 (Figs. 3 and 4).Fig. 4

Bottom Line: We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval.Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. sadoneli@gmail.com.

ABSTRACT

Background: Stripe rust, a highly destructive foliar disease of wheat (Triticum aestivum), causes severe losses, which may be accompanied by reduced photosynthetic activity and accelerated leaf senescence.

Methods: We used suppression subtractive hybridization (SSH) to examine the mechanisms of resistance in the resistant wheat line L693 (Reg. No. GP-972, PI 672538), which was derived from a lineage that includes a wide cross between common and Thinopyrum intermedium. Sequencing of an SSH cDNA library identified 112 expressed sequence tags.

Results: In silico mapping placed one of these tags [GenBank: JK972238] on chromosome 1A. Primers based on [GenBank: JK972238] amplified a polymorphic band, which co-segregated with YrL693. We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval. Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.

Discussion: Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.

Conclusions: The unique chromosomal location and expression mode of WCBP1 suggested that WCBP1 is the putative candidate gene of YrL693, which was involved in leaf senescence and photosynthesis related to plant responses to stripe rust infection during the grain-filling stage.

No MeSH data available.


Related in: MedlinePlus