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Wheat WCBP1 encodes a putative copper-binding protein involved in stripe rust resistance and inhibition of leaf senescence.

Li X, Liu T, Chen W, Zhong S, Zhang H, Tang Z, Chang Z, Wang L, Zhang M, Li L, Rao H, Ren Z, Luo P - BMC Plant Biol. (2015)

Bottom Line: We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval.Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. sadoneli@gmail.com.

ABSTRACT

Background: Stripe rust, a highly destructive foliar disease of wheat (Triticum aestivum), causes severe losses, which may be accompanied by reduced photosynthetic activity and accelerated leaf senescence.

Methods: We used suppression subtractive hybridization (SSH) to examine the mechanisms of resistance in the resistant wheat line L693 (Reg. No. GP-972, PI 672538), which was derived from a lineage that includes a wide cross between common and Thinopyrum intermedium. Sequencing of an SSH cDNA library identified 112 expressed sequence tags.

Results: In silico mapping placed one of these tags [GenBank: JK972238] on chromosome 1A. Primers based on [GenBank: JK972238] amplified a polymorphic band, which co-segregated with YrL693. We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval. Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.

Discussion: Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.

Conclusions: The unique chromosomal location and expression mode of WCBP1 suggested that WCBP1 is the putative candidate gene of YrL693, which was involved in leaf senescence and photosynthesis related to plant responses to stripe rust infection during the grain-filling stage.

No MeSH data available.


Related in: MedlinePlus

Seedling (a) and adult (b) responses to a) L693, b) L661, c) L693, d) L661. c Functional classes of differentially expressed sequences induced by Pst inoculation
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Fig1: Seedling (a) and adult (b) responses to a) L693, b) L661, c) L693, d) L661. c Functional classes of differentially expressed sequences induced by Pst inoculation

Mentions: L693 was highly resistant and L661 highly susceptible to Pst race CYR32 (Fig. 1a). The response tests performed at the adult (Fig. 1b) and seedling stages (Fig. 1a) showed similar results. Random sequencing of bacterial colonies from the suppression subtractive hybridization (SSH) library identified 112 ESTs, with an average length of 178 bp, which were submitted to NCBI under the accession numbers [GenBank: JK972179] though [GenBank: JK972287] and [GenBank: JK974072] through [GenBank: JK974074] (Additional file 2: Table S1). Of these, 87 unique ESTs (uniESTs) with an average length of 197 bp (Table 1), including 21 contigs with an average length of 226 bp and 66 singletons with an average length of 188 bp, were found. Most of the uniESTs were between 50 bp and 300 bp in length (Additional file 1: Figure S2) and were further identified through clustering and assembly of putative full-length cDNAs based on the BLAST analysis option in NCBI GenBank (Additional file 2: Table S2).Fig. 1


Wheat WCBP1 encodes a putative copper-binding protein involved in stripe rust resistance and inhibition of leaf senescence.

Li X, Liu T, Chen W, Zhong S, Zhang H, Tang Z, Chang Z, Wang L, Zhang M, Li L, Rao H, Ren Z, Luo P - BMC Plant Biol. (2015)

Seedling (a) and adult (b) responses to a) L693, b) L661, c) L693, d) L661. c Functional classes of differentially expressed sequences induced by Pst inoculation
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4595213&req=5

Fig1: Seedling (a) and adult (b) responses to a) L693, b) L661, c) L693, d) L661. c Functional classes of differentially expressed sequences induced by Pst inoculation
Mentions: L693 was highly resistant and L661 highly susceptible to Pst race CYR32 (Fig. 1a). The response tests performed at the adult (Fig. 1b) and seedling stages (Fig. 1a) showed similar results. Random sequencing of bacterial colonies from the suppression subtractive hybridization (SSH) library identified 112 ESTs, with an average length of 178 bp, which were submitted to NCBI under the accession numbers [GenBank: JK972179] though [GenBank: JK972287] and [GenBank: JK974072] through [GenBank: JK974074] (Additional file 2: Table S1). Of these, 87 unique ESTs (uniESTs) with an average length of 197 bp (Table 1), including 21 contigs with an average length of 226 bp and 66 singletons with an average length of 188 bp, were found. Most of the uniESTs were between 50 bp and 300 bp in length (Additional file 1: Figure S2) and were further identified through clustering and assembly of putative full-length cDNAs based on the BLAST analysis option in NCBI GenBank (Additional file 2: Table S2).Fig. 1

Bottom Line: We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval.Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. sadoneli@gmail.com.

ABSTRACT

Background: Stripe rust, a highly destructive foliar disease of wheat (Triticum aestivum), causes severe losses, which may be accompanied by reduced photosynthetic activity and accelerated leaf senescence.

Methods: We used suppression subtractive hybridization (SSH) to examine the mechanisms of resistance in the resistant wheat line L693 (Reg. No. GP-972, PI 672538), which was derived from a lineage that includes a wide cross between common and Thinopyrum intermedium. Sequencing of an SSH cDNA library identified 112 expressed sequence tags.

Results: In silico mapping placed one of these tags [GenBank: JK972238] on chromosome 1A. Primers based on [GenBank: JK972238] amplified a polymorphic band, which co-segregated with YrL693. We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval. Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.

Discussion: Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.

Conclusions: The unique chromosomal location and expression mode of WCBP1 suggested that WCBP1 is the putative candidate gene of YrL693, which was involved in leaf senescence and photosynthesis related to plant responses to stripe rust infection during the grain-filling stage.

No MeSH data available.


Related in: MedlinePlus