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New circulating biomarkers for predicting cardiovascular death in healthy population.

Melander O, Modrego J, Zamorano-León JJ, Santos-Sancho JM, Lahera V, López-Farré AJ - J. Cell. Mol. Med. (2015)

Bottom Line: To determine in healthy individuals new circulating protein biomarkers, whose systemic levels may be associated with the risk of future development of CVD incidents and death.There were proteins whose expression in plasma was significantly higher in participants suffering CVD death as compared with those that did not die by CVD.Several protein plasma levels and protein isotypes related to inflammation and thrombo-coagulating phenomena were independently associated with the risk of future CVD death.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Sciences, Lund University, Malmö, Sweden.

No MeSH data available.


Related in: MedlinePlus

On the upper left side is shown a representative dot-blot to determine plasma vitamin D-binding protein (DBP) and fibrinogen-γ-chain expression in participants with and without cardiovascular death during follow-up. In the images is also shown representatives expression of β-actin in plasma that was used as loading protein control and for to normalize the densitometric analysis in dot-blots. The bar graphs show the densitometric analysis of all dot-blots in arbitrary units (A.U.). Densitometric values were normalysed by plasma β-actin expression and are represented as mean ± SEM. *P < 0.05 with respect to participants with non-cardiovascular death during follow-up. On the upper right side is shown a representative Western-blot showing single bands of 55 and 57 kD of apparent molecular weights corresponding to DBP and fibrinogen-γ-chain respectively.
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fig03: On the upper left side is shown a representative dot-blot to determine plasma vitamin D-binding protein (DBP) and fibrinogen-γ-chain expression in participants with and without cardiovascular death during follow-up. In the images is also shown representatives expression of β-actin in plasma that was used as loading protein control and for to normalize the densitometric analysis in dot-blots. The bar graphs show the densitometric analysis of all dot-blots in arbitrary units (A.U.). Densitometric values were normalysed by plasma β-actin expression and are represented as mean ± SEM. *P < 0.05 with respect to participants with non-cardiovascular death during follow-up. On the upper right side is shown a representative Western-blot showing single bands of 55 and 57 kD of apparent molecular weights corresponding to DBP and fibrinogen-γ-chain respectively.

Mentions: The association of the increased plasma expression of some of these proteins with future cardiovascular death was also analysed using dot-blot analysis. As Figure 3 shows, dot-blot analysis demonstrated that total expression of both DBP and fibrinogen-γ-chain was significantly higher in participants with cardiovascular death than in those with non-cardiovascular death during follow-up. Western blot analysis supported that the antibodies used in dot-blots recognized single bands of apparent molecular weight around 55 kD for DBP and 57 kD for fibrinogen-γ-chain, corresponding to the reported molecular weight for each of these proteins (Fig. 3).


New circulating biomarkers for predicting cardiovascular death in healthy population.

Melander O, Modrego J, Zamorano-León JJ, Santos-Sancho JM, Lahera V, López-Farré AJ - J. Cell. Mol. Med. (2015)

On the upper left side is shown a representative dot-blot to determine plasma vitamin D-binding protein (DBP) and fibrinogen-γ-chain expression in participants with and without cardiovascular death during follow-up. In the images is also shown representatives expression of β-actin in plasma that was used as loading protein control and for to normalize the densitometric analysis in dot-blots. The bar graphs show the densitometric analysis of all dot-blots in arbitrary units (A.U.). Densitometric values were normalysed by plasma β-actin expression and are represented as mean ± SEM. *P < 0.05 with respect to participants with non-cardiovascular death during follow-up. On the upper right side is shown a representative Western-blot showing single bands of 55 and 57 kD of apparent molecular weights corresponding to DBP and fibrinogen-γ-chain respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4594690&req=5

fig03: On the upper left side is shown a representative dot-blot to determine plasma vitamin D-binding protein (DBP) and fibrinogen-γ-chain expression in participants with and without cardiovascular death during follow-up. In the images is also shown representatives expression of β-actin in plasma that was used as loading protein control and for to normalize the densitometric analysis in dot-blots. The bar graphs show the densitometric analysis of all dot-blots in arbitrary units (A.U.). Densitometric values were normalysed by plasma β-actin expression and are represented as mean ± SEM. *P < 0.05 with respect to participants with non-cardiovascular death during follow-up. On the upper right side is shown a representative Western-blot showing single bands of 55 and 57 kD of apparent molecular weights corresponding to DBP and fibrinogen-γ-chain respectively.
Mentions: The association of the increased plasma expression of some of these proteins with future cardiovascular death was also analysed using dot-blot analysis. As Figure 3 shows, dot-blot analysis demonstrated that total expression of both DBP and fibrinogen-γ-chain was significantly higher in participants with cardiovascular death than in those with non-cardiovascular death during follow-up. Western blot analysis supported that the antibodies used in dot-blots recognized single bands of apparent molecular weight around 55 kD for DBP and 57 kD for fibrinogen-γ-chain, corresponding to the reported molecular weight for each of these proteins (Fig. 3).

Bottom Line: To determine in healthy individuals new circulating protein biomarkers, whose systemic levels may be associated with the risk of future development of CVD incidents and death.There were proteins whose expression in plasma was significantly higher in participants suffering CVD death as compared with those that did not die by CVD.Several protein plasma levels and protein isotypes related to inflammation and thrombo-coagulating phenomena were independently associated with the risk of future CVD death.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Sciences, Lund University, Malmö, Sweden.

No MeSH data available.


Related in: MedlinePlus