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Protective Role of Aerobic Exercise Against Cisplatin-Induced Nephrotoxicity in Rats.

Zeynali F, Nematbakhsh M, Mojtahedi H, Poorshahnazari A, Talebi A, Pezeshki Z, Mazaheri S, Moslemi F - Asian J Sports Med (2015)

Bottom Line: Finally, the animals were sacrificed for the biochemical measurement and tissue histopathology investigation.Kidney and serum levels of MDA and nitrite did not alter significantly.Also, the severity of kidney tissue damage decreased significantly in groups I and II (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, IR Iran ; Department of Physical Education, Faculty of Physical Education, Isfahan University, Isfahan, IR Iran.

ABSTRACT

Background: Cisplatin (CP) is a chemotherapy drug and nephrotoxicity is considered as its major side effect. Aerobic exercise is well known as an approach to reduce the side effects of many drugs.

Objectives: This study was designed to determine the protective role of aerobic exercise against CP-induced nephrotoxicity.

Materials and methods: Thirty male Wistar rats were randomly divided into four groups. Group I had aerobic exercise on a treadmill one hour per day and five days per week for eight weeks. Then, the exercise protocol was continued for another week, but during this week, the animals also received CP (2.5 mg/kg/day; ip). Group II underwent the same protocol as group I without exercise in the last week during the CP therapy. Groups III and IV were assigned as positive and negative control groups, and were treated with CP and saline without exercise, respectively. Finally, the animals were sacrificed for the biochemical measurement and tissue histopathology investigation.

Results: CP alone without exercise increased serum levels of blood urea nitrogen (BUN), creatinine (Cr), and malondialdehyde (MDA); and kidney nitrite level, while treadmill exercise in group I significantly ameliorated these parameters (P < 0.05). Kidney and serum levels of MDA and nitrite did not alter significantly. Also, the severity of kidney tissue damage decreased significantly in groups I and II (P < 0.05).

Conclusions: Aerobic exercise may reduce CP-induced nephrotoxicity with a favorable effect on renal function by increasing activation of antioxidant system.

No MeSH data available.


Related in: MedlinePlus

The Images (100×) of Kidney Tissue Stained With Hande to Examine Tissue Damage in the Kidney of The Four Experimental GroupsThe arrow shows the kidney tissue damage. CP, cisplatin; EX, exercise
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fig19783: The Images (100×) of Kidney Tissue Stained With Hande to Examine Tissue Damage in the Kidney of The Four Experimental GroupsThe arrow shows the kidney tissue damage. CP, cisplatin; EX, exercise

Mentions: CP alone reduced BW in the positive control group when compared with the negative control group (sham) (P < 0.05). Exercise had no significant effect on BW. The KW significantly increased in the positive control group when compared with the sham group (P < 0.05). No significant changes in KW were observed in groups I and II in comparison with the negative and positive control groups. KTDS was evaluated and scored by a pathologist. Higher kidney damage score was detected in the positive control group when compared with the negative control group (P < 0.05). The KTDS in EX + CP + EX and EX + CP groups significantly reduced when compared with the positive control group (P < 0.05) (Figure 1). The images of kidney tissues is shown in Figure 2.


Protective Role of Aerobic Exercise Against Cisplatin-Induced Nephrotoxicity in Rats.

Zeynali F, Nematbakhsh M, Mojtahedi H, Poorshahnazari A, Talebi A, Pezeshki Z, Mazaheri S, Moslemi F - Asian J Sports Med (2015)

The Images (100×) of Kidney Tissue Stained With Hande to Examine Tissue Damage in the Kidney of The Four Experimental GroupsThe arrow shows the kidney tissue damage. CP, cisplatin; EX, exercise
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4594143&req=5

fig19783: The Images (100×) of Kidney Tissue Stained With Hande to Examine Tissue Damage in the Kidney of The Four Experimental GroupsThe arrow shows the kidney tissue damage. CP, cisplatin; EX, exercise
Mentions: CP alone reduced BW in the positive control group when compared with the negative control group (sham) (P < 0.05). Exercise had no significant effect on BW. The KW significantly increased in the positive control group when compared with the sham group (P < 0.05). No significant changes in KW were observed in groups I and II in comparison with the negative and positive control groups. KTDS was evaluated and scored by a pathologist. Higher kidney damage score was detected in the positive control group when compared with the negative control group (P < 0.05). The KTDS in EX + CP + EX and EX + CP groups significantly reduced when compared with the positive control group (P < 0.05) (Figure 1). The images of kidney tissues is shown in Figure 2.

Bottom Line: Finally, the animals were sacrificed for the biochemical measurement and tissue histopathology investigation.Kidney and serum levels of MDA and nitrite did not alter significantly.Also, the severity of kidney tissue damage decreased significantly in groups I and II (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, IR Iran ; Department of Physical Education, Faculty of Physical Education, Isfahan University, Isfahan, IR Iran.

ABSTRACT

Background: Cisplatin (CP) is a chemotherapy drug and nephrotoxicity is considered as its major side effect. Aerobic exercise is well known as an approach to reduce the side effects of many drugs.

Objectives: This study was designed to determine the protective role of aerobic exercise against CP-induced nephrotoxicity.

Materials and methods: Thirty male Wistar rats were randomly divided into four groups. Group I had aerobic exercise on a treadmill one hour per day and five days per week for eight weeks. Then, the exercise protocol was continued for another week, but during this week, the animals also received CP (2.5 mg/kg/day; ip). Group II underwent the same protocol as group I without exercise in the last week during the CP therapy. Groups III and IV were assigned as positive and negative control groups, and were treated with CP and saline without exercise, respectively. Finally, the animals were sacrificed for the biochemical measurement and tissue histopathology investigation.

Results: CP alone without exercise increased serum levels of blood urea nitrogen (BUN), creatinine (Cr), and malondialdehyde (MDA); and kidney nitrite level, while treadmill exercise in group I significantly ameliorated these parameters (P < 0.05). Kidney and serum levels of MDA and nitrite did not alter significantly. Also, the severity of kidney tissue damage decreased significantly in groups I and II (P < 0.05).

Conclusions: Aerobic exercise may reduce CP-induced nephrotoxicity with a favorable effect on renal function by increasing activation of antioxidant system.

No MeSH data available.


Related in: MedlinePlus