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Antimicrobial activity of iron oxide nanoparticle upon modulation of nanoparticle-bacteria interface.

Arakha M, Pal S, Samantarrai D, Panigrahi TK, Mallick BC, Pramanik K, Mallick B, Jha S - Sci Rep (2015)

Bottom Line: Additionally, the nanocrystals obtained were found to have spherical size with 10-20 nm diameter.However, coating with chitosan molecule resulted significant increase in antimicrobial propensity of IONP.The data, altogether, indicated that the chitosan coating of IONP result in interface that enhances ROS production, hence the antimicrobial activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.

ABSTRACT
Investigating the interaction patterns at nano-bio interface is a key challenge for safe use of nanoparticles (NPs) to any biological system. The study intends to explore the role of interaction pattern at the iron oxide nanoparticle (IONP)-bacteria interface affecting antimicrobial propensity of IONP. To this end, IONP with magnetite like atomic arrangement and negative surface potential (n-IONP) was synthesized by co-precipitation method. Positively charged chitosan molecule coating was used to reverse the surface potential of n-IONP, i.e. positive surface potential IONP (p-IONP). The comparative data from fourier transform infrared spectroscope, XRD, and zeta potential analyzer indicated the successful coating of IONP surface with chitosan molecule. Additionally, the nanocrystals obtained were found to have spherical size with 10-20 nm diameter. The BacLight fluorescence assay, bacterial growth kinetic and colony forming unit studies indicated that n-IONP (<50 μM) has insignificant antimicrobial activity against Bacillus subtilis and Escherichia coli. However, coating with chitosan molecule resulted significant increase in antimicrobial propensity of IONP. Additionally, the assay to study reactive oxygen species (ROS) indicated relatively higher ROS production upon p-IONP treatment of the bacteria. The data, altogether, indicated that the chitosan coating of IONP result in interface that enhances ROS production, hence the antimicrobial activity.

No MeSH data available.


Related in: MedlinePlus

Growth kinetics of B. subtilis (Figs. 3a and 3b) and E. coli (Figs. 3c and 3d) in absence and presence of different concentrations of n-IONP (Fig. 3a for B. subtilis and 3c for E. coli) & p-IONP (Fig. 3b for B. subtilis and 3d for E. coli).Different concentrations of the NPs taken were 2.5, 5, 10, 25, and 50 μM, and injected at the log phase of growth kinetics (shown by arrow). Triplicate experiments were done for each reaction, and the error bar represents the standard error of mean.
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f3: Growth kinetics of B. subtilis (Figs. 3a and 3b) and E. coli (Figs. 3c and 3d) in absence and presence of different concentrations of n-IONP (Fig. 3a for B. subtilis and 3c for E. coli) & p-IONP (Fig. 3b for B. subtilis and 3d for E. coli).Different concentrations of the NPs taken were 2.5, 5, 10, 25, and 50 μM, and injected at the log phase of growth kinetics (shown by arrow). Triplicate experiments were done for each reaction, and the error bar represents the standard error of mean.

Mentions: The growth kinetic studies of B. subtilis and E. coli in presence of different concentrations of n-IONP and p-IONP are shown in Fig. 3. Figure 3a,c display the growth curve of B. subtilis and E. coli, respectively, in presence of different concentrations of n-IONP. As shown in the figures, insignificant growth inhibition compared to control were observed for the studied concentrations of n-IONP, whereas the inhibition is relatively very dominant for B. subtilis (Fig. 3b) and E. coli (Fig. 3d) in presence of relative p-IONP concentrations. However, CFU measurements indicate the antimicrobial activity of n-IONP at higher concentrations (Fig. 4). The viability of both bacterial cells reduced by approximately 30% in presence of 50 μM of n-IONP. However, the coated IONP has significant effect on bacterial viability, viability reduced by 70% in presence of 50 μM p-IONP. The data indicates strong antimicrobial propensity of p-IONP against studied bacterial strains. Additionally, the data support the growth kinetic studies observed in presence of n-IONP and p-IONP.


Antimicrobial activity of iron oxide nanoparticle upon modulation of nanoparticle-bacteria interface.

Arakha M, Pal S, Samantarrai D, Panigrahi TK, Mallick BC, Pramanik K, Mallick B, Jha S - Sci Rep (2015)

Growth kinetics of B. subtilis (Figs. 3a and 3b) and E. coli (Figs. 3c and 3d) in absence and presence of different concentrations of n-IONP (Fig. 3a for B. subtilis and 3c for E. coli) & p-IONP (Fig. 3b for B. subtilis and 3d for E. coli).Different concentrations of the NPs taken were 2.5, 5, 10, 25, and 50 μM, and injected at the log phase of growth kinetics (shown by arrow). Triplicate experiments were done for each reaction, and the error bar represents the standard error of mean.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4594095&req=5

f3: Growth kinetics of B. subtilis (Figs. 3a and 3b) and E. coli (Figs. 3c and 3d) in absence and presence of different concentrations of n-IONP (Fig. 3a for B. subtilis and 3c for E. coli) & p-IONP (Fig. 3b for B. subtilis and 3d for E. coli).Different concentrations of the NPs taken were 2.5, 5, 10, 25, and 50 μM, and injected at the log phase of growth kinetics (shown by arrow). Triplicate experiments were done for each reaction, and the error bar represents the standard error of mean.
Mentions: The growth kinetic studies of B. subtilis and E. coli in presence of different concentrations of n-IONP and p-IONP are shown in Fig. 3. Figure 3a,c display the growth curve of B. subtilis and E. coli, respectively, in presence of different concentrations of n-IONP. As shown in the figures, insignificant growth inhibition compared to control were observed for the studied concentrations of n-IONP, whereas the inhibition is relatively very dominant for B. subtilis (Fig. 3b) and E. coli (Fig. 3d) in presence of relative p-IONP concentrations. However, CFU measurements indicate the antimicrobial activity of n-IONP at higher concentrations (Fig. 4). The viability of both bacterial cells reduced by approximately 30% in presence of 50 μM of n-IONP. However, the coated IONP has significant effect on bacterial viability, viability reduced by 70% in presence of 50 μM p-IONP. The data indicates strong antimicrobial propensity of p-IONP against studied bacterial strains. Additionally, the data support the growth kinetic studies observed in presence of n-IONP and p-IONP.

Bottom Line: Additionally, the nanocrystals obtained were found to have spherical size with 10-20 nm diameter.However, coating with chitosan molecule resulted significant increase in antimicrobial propensity of IONP.The data, altogether, indicated that the chitosan coating of IONP result in interface that enhances ROS production, hence the antimicrobial activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.

ABSTRACT
Investigating the interaction patterns at nano-bio interface is a key challenge for safe use of nanoparticles (NPs) to any biological system. The study intends to explore the role of interaction pattern at the iron oxide nanoparticle (IONP)-bacteria interface affecting antimicrobial propensity of IONP. To this end, IONP with magnetite like atomic arrangement and negative surface potential (n-IONP) was synthesized by co-precipitation method. Positively charged chitosan molecule coating was used to reverse the surface potential of n-IONP, i.e. positive surface potential IONP (p-IONP). The comparative data from fourier transform infrared spectroscope, XRD, and zeta potential analyzer indicated the successful coating of IONP surface with chitosan molecule. Additionally, the nanocrystals obtained were found to have spherical size with 10-20 nm diameter. The BacLight fluorescence assay, bacterial growth kinetic and colony forming unit studies indicated that n-IONP (<50 μM) has insignificant antimicrobial activity against Bacillus subtilis and Escherichia coli. However, coating with chitosan molecule resulted significant increase in antimicrobial propensity of IONP. Additionally, the assay to study reactive oxygen species (ROS) indicated relatively higher ROS production upon p-IONP treatment of the bacteria. The data, altogether, indicated that the chitosan coating of IONP result in interface that enhances ROS production, hence the antimicrobial activity.

No MeSH data available.


Related in: MedlinePlus