Limits...
Antimicrobial activity of iron oxide nanoparticle upon modulation of nanoparticle-bacteria interface.

Arakha M, Pal S, Samantarrai D, Panigrahi TK, Mallick BC, Pramanik K, Mallick B, Jha S - Sci Rep (2015)

Bottom Line: Additionally, the nanocrystals obtained were found to have spherical size with 10-20 nm diameter.However, coating with chitosan molecule resulted significant increase in antimicrobial propensity of IONP.The data, altogether, indicated that the chitosan coating of IONP result in interface that enhances ROS production, hence the antimicrobial activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.

ABSTRACT
Investigating the interaction patterns at nano-bio interface is a key challenge for safe use of nanoparticles (NPs) to any biological system. The study intends to explore the role of interaction pattern at the iron oxide nanoparticle (IONP)-bacteria interface affecting antimicrobial propensity of IONP. To this end, IONP with magnetite like atomic arrangement and negative surface potential (n-IONP) was synthesized by co-precipitation method. Positively charged chitosan molecule coating was used to reverse the surface potential of n-IONP, i.e. positive surface potential IONP (p-IONP). The comparative data from fourier transform infrared spectroscope, XRD, and zeta potential analyzer indicated the successful coating of IONP surface with chitosan molecule. Additionally, the nanocrystals obtained were found to have spherical size with 10-20 nm diameter. The BacLight fluorescence assay, bacterial growth kinetic and colony forming unit studies indicated that n-IONP (<50 μM) has insignificant antimicrobial activity against Bacillus subtilis and Escherichia coli. However, coating with chitosan molecule resulted significant increase in antimicrobial propensity of IONP. Additionally, the assay to study reactive oxygen species (ROS) indicated relatively higher ROS production upon p-IONP treatment of the bacteria. The data, altogether, indicated that the chitosan coating of IONP result in interface that enhances ROS production, hence the antimicrobial activity.

No MeSH data available.


Related in: MedlinePlus

FE-SEM image of n-IONP (Fig. 2a) and p-IONP (Fig. 2b).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4594095&req=5

f2: FE-SEM image of n-IONP (Fig. 2a) and p-IONP (Fig. 2b).

Mentions: The images obtained using FE-SEM, Fig. 2, indicate that both types of IONPs are nearly spherical in shape with size ranging from 8–20 nm, which is very close to the estimated particle size by XRD analysis, i.e. ~11 +/− 5 nm. Both FE-SEM analysis and XRD analysis suggest that the size of the NPs does not change significantly upon chitosan coating. Yu S. et al. have reported a similar observation, 8 nm IONP retained its size on coating with poly-(methacrylic acid)28. Additionally, slight difference in particle size for n-IONP and p-IONP may be dedicated to instrumental artefact or sampling artefact or both.


Antimicrobial activity of iron oxide nanoparticle upon modulation of nanoparticle-bacteria interface.

Arakha M, Pal S, Samantarrai D, Panigrahi TK, Mallick BC, Pramanik K, Mallick B, Jha S - Sci Rep (2015)

FE-SEM image of n-IONP (Fig. 2a) and p-IONP (Fig. 2b).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4594095&req=5

f2: FE-SEM image of n-IONP (Fig. 2a) and p-IONP (Fig. 2b).
Mentions: The images obtained using FE-SEM, Fig. 2, indicate that both types of IONPs are nearly spherical in shape with size ranging from 8–20 nm, which is very close to the estimated particle size by XRD analysis, i.e. ~11 +/− 5 nm. Both FE-SEM analysis and XRD analysis suggest that the size of the NPs does not change significantly upon chitosan coating. Yu S. et al. have reported a similar observation, 8 nm IONP retained its size on coating with poly-(methacrylic acid)28. Additionally, slight difference in particle size for n-IONP and p-IONP may be dedicated to instrumental artefact or sampling artefact or both.

Bottom Line: Additionally, the nanocrystals obtained were found to have spherical size with 10-20 nm diameter.However, coating with chitosan molecule resulted significant increase in antimicrobial propensity of IONP.The data, altogether, indicated that the chitosan coating of IONP result in interface that enhances ROS production, hence the antimicrobial activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.

ABSTRACT
Investigating the interaction patterns at nano-bio interface is a key challenge for safe use of nanoparticles (NPs) to any biological system. The study intends to explore the role of interaction pattern at the iron oxide nanoparticle (IONP)-bacteria interface affecting antimicrobial propensity of IONP. To this end, IONP with magnetite like atomic arrangement and negative surface potential (n-IONP) was synthesized by co-precipitation method. Positively charged chitosan molecule coating was used to reverse the surface potential of n-IONP, i.e. positive surface potential IONP (p-IONP). The comparative data from fourier transform infrared spectroscope, XRD, and zeta potential analyzer indicated the successful coating of IONP surface with chitosan molecule. Additionally, the nanocrystals obtained were found to have spherical size with 10-20 nm diameter. The BacLight fluorescence assay, bacterial growth kinetic and colony forming unit studies indicated that n-IONP (<50 μM) has insignificant antimicrobial activity against Bacillus subtilis and Escherichia coli. However, coating with chitosan molecule resulted significant increase in antimicrobial propensity of IONP. Additionally, the assay to study reactive oxygen species (ROS) indicated relatively higher ROS production upon p-IONP treatment of the bacteria. The data, altogether, indicated that the chitosan coating of IONP result in interface that enhances ROS production, hence the antimicrobial activity.

No MeSH data available.


Related in: MedlinePlus