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Synthesis, characterization, and cytotoxicity in human erythrocytes of multifunctional, magnetic, and luminescent nanocrystalline rare earth fluorides.

Grzyb T, Mrówczyńska L, Szczeszak A, Śniadecki Z, Runowski M, Idzikowski B, Lis S - J Nanopart Res (2015)

Bottom Line: The highest luminescence was observed for BaGdF5-based materials.The particles' magnetic characteristics were also preserved for samples in the form of a suspension in distilled water.The cytotoxicity studies against the human erythrocytes indicated that the synthesized nanoparticles are non-toxic because they did not cause the red blood cells shape changes nor did they alter their membrane structure and permeabilization.

View Article: PubMed Central - PubMed

Affiliation: Department of Rare Earths, Faculty of Chemistry, Adam Mickiewicz University, Umultowska 89b, 61-614 Poznan, Poland.

ABSTRACT

Multifunctional nanoparticles exhibiting red or green luminescence properties and magnetism were synthesized and thoroughly analyzed. The hydrothermal method was used for the synthesis of Eu(3+)- or Tb(3+)-doped GdF3-, NaGdF4-, and BaGdF5-based nanocrystalline materials. The X-ray diffraction patterns of the samples confirmed the desired compositions of the materials. Transmission electron microscope images revealed the different morphologies of the products, including the nanocrystal sizes, which varied from 12 nm in the case of BaGdF5-based nanoparticles to larger structures with dimensions exceeding 300 nm. All of the samples presented luminescence under ultraviolet irradiation, as well as when the samples were in the form of water colloids. The highest luminescence was observed for BaGdF5-based materials. The obtained nanoparticles exhibited paramagnetism along with probable evidence of superparamagnetic behavior at low temperatures. The particles' magnetic characteristics were also preserved for samples in the form of a suspension in distilled water. The cytotoxicity studies against the human erythrocytes indicated that the synthesized nanoparticles are non-toxic because they did not cause the red blood cells shape changes nor did they alter their membrane structure and permeabilization.

No MeSH data available.


Related in: MedlinePlus

a, b, c The effect of PBS and d, e, f GdF3: 2.5 %Ce3+,2.5 %Tb3+ (NaBF4) at 5 mg/mL (1 h, 37 °C) on human erythrocytes, as observed using a fluorescence microscope. (a and d) argon laser wavelength of 488 and LP505 nm, (b and e) argon laser wavelength of 488 nm and BP 565–615 nm, (c and f) argon laser wavelength of 458 nm and BP 480–520 nm. No noticeable changes were observed between the autofluorescence of the control erythrocytes (a, b, c) and the erythrocytes exposed to nanoparticles (d, e, f). Scale bars indicate 10 µm
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Fig9: a, b, c The effect of PBS and d, e, f GdF3: 2.5 %Ce3+,2.5 %Tb3+ (NaBF4) at 5 mg/mL (1 h, 37 °C) on human erythrocytes, as observed using a fluorescence microscope. (a and d) argon laser wavelength of 488 and LP505 nm, (b and e) argon laser wavelength of 488 nm and BP 565–615 nm, (c and f) argon laser wavelength of 458 nm and BP 480–520 nm. No noticeable changes were observed between the autofluorescence of the control erythrocytes (a, b, c) and the erythrocytes exposed to nanoparticles (d, e, f). Scale bars indicate 10 µm

Mentions: We applied fluorescence microscopic techniques to visualize the nanoparticles attached to the RBC membrane. Unfortunately, the fluorescence signals of the RBCs treated with nanoparticles (Fig. 9d–f) and the RBCs incubated without (Fig. 9a–c) were found to be similar. Therefore, the precise detection of RBC membrane-bound nanoparticles using fluorescence microscopy techniques was not possible.Fig. 9


Synthesis, characterization, and cytotoxicity in human erythrocytes of multifunctional, magnetic, and luminescent nanocrystalline rare earth fluorides.

Grzyb T, Mrówczyńska L, Szczeszak A, Śniadecki Z, Runowski M, Idzikowski B, Lis S - J Nanopart Res (2015)

a, b, c The effect of PBS and d, e, f GdF3: 2.5 %Ce3+,2.5 %Tb3+ (NaBF4) at 5 mg/mL (1 h, 37 °C) on human erythrocytes, as observed using a fluorescence microscope. (a and d) argon laser wavelength of 488 and LP505 nm, (b and e) argon laser wavelength of 488 nm and BP 565–615 nm, (c and f) argon laser wavelength of 458 nm and BP 480–520 nm. No noticeable changes were observed between the autofluorescence of the control erythrocytes (a, b, c) and the erythrocytes exposed to nanoparticles (d, e, f). Scale bars indicate 10 µm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4594090&req=5

Fig9: a, b, c The effect of PBS and d, e, f GdF3: 2.5 %Ce3+,2.5 %Tb3+ (NaBF4) at 5 mg/mL (1 h, 37 °C) on human erythrocytes, as observed using a fluorescence microscope. (a and d) argon laser wavelength of 488 and LP505 nm, (b and e) argon laser wavelength of 488 nm and BP 565–615 nm, (c and f) argon laser wavelength of 458 nm and BP 480–520 nm. No noticeable changes were observed between the autofluorescence of the control erythrocytes (a, b, c) and the erythrocytes exposed to nanoparticles (d, e, f). Scale bars indicate 10 µm
Mentions: We applied fluorescence microscopic techniques to visualize the nanoparticles attached to the RBC membrane. Unfortunately, the fluorescence signals of the RBCs treated with nanoparticles (Fig. 9d–f) and the RBCs incubated without (Fig. 9a–c) were found to be similar. Therefore, the precise detection of RBC membrane-bound nanoparticles using fluorescence microscopy techniques was not possible.Fig. 9

Bottom Line: The highest luminescence was observed for BaGdF5-based materials.The particles' magnetic characteristics were also preserved for samples in the form of a suspension in distilled water.The cytotoxicity studies against the human erythrocytes indicated that the synthesized nanoparticles are non-toxic because they did not cause the red blood cells shape changes nor did they alter their membrane structure and permeabilization.

View Article: PubMed Central - PubMed

Affiliation: Department of Rare Earths, Faculty of Chemistry, Adam Mickiewicz University, Umultowska 89b, 61-614 Poznan, Poland.

ABSTRACT

Multifunctional nanoparticles exhibiting red or green luminescence properties and magnetism were synthesized and thoroughly analyzed. The hydrothermal method was used for the synthesis of Eu(3+)- or Tb(3+)-doped GdF3-, NaGdF4-, and BaGdF5-based nanocrystalline materials. The X-ray diffraction patterns of the samples confirmed the desired compositions of the materials. Transmission electron microscope images revealed the different morphologies of the products, including the nanocrystal sizes, which varied from 12 nm in the case of BaGdF5-based nanoparticles to larger structures with dimensions exceeding 300 nm. All of the samples presented luminescence under ultraviolet irradiation, as well as when the samples were in the form of water colloids. The highest luminescence was observed for BaGdF5-based materials. The obtained nanoparticles exhibited paramagnetism along with probable evidence of superparamagnetic behavior at low temperatures. The particles' magnetic characteristics were also preserved for samples in the form of a suspension in distilled water. The cytotoxicity studies against the human erythrocytes indicated that the synthesized nanoparticles are non-toxic because they did not cause the red blood cells shape changes nor did they alter their membrane structure and permeabilization.

No MeSH data available.


Related in: MedlinePlus