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A Nanoparticle-based Sensor Platform for Cell Tracking and Status/Function Assessment.

Yeo D, Wiraja C, Chuah YJ, Gao Y, Xu C - Sci Rep (2015)

Bottom Line: Upon intracellular entry, nanosensors reside within the cell cytoplasm, serving as a depot to continuously release sensor molecules for up to 30 days.When the biomarker(s) is expressed, a detectable signal is generated (On).As a proof-of-concept, three nanosensor formulations were synthesized to monitor cell viability, secretion of nitric oxide, and β-actin mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical &Biomedical Engineering, Nanyang Technological University, Singapore.

ABSTRACT
Nanoparticles are increasingly popular choices for labeling and tracking cells in biomedical applications such as cell therapy. However, all current types of nanoparticles fail to provide real-time, noninvasive monitoring of cell status and functions while often generating false positive signals. Herein, a nanosensor platform to track the real-time expression of specific biomarkers that correlate with cell status and functions is reported. Nanosensors are synthesized by encapsulating various sensor molecules within biodegradable polymeric nanoparticles. Upon intracellular entry, nanosensors reside within the cell cytoplasm, serving as a depot to continuously release sensor molecules for up to 30 days. In the absence of the target biomarkers, the released sensor molecules remain 'Off'. When the biomarker(s) is expressed, a detectable signal is generated (On). As a proof-of-concept, three nanosensor formulations were synthesized to monitor cell viability, secretion of nitric oxide, and β-actin mRNA expression.

No MeSH data available.


Related in: MedlinePlus

Schematic illustration of the nanosensor platform and intracellular implementation.
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f1: Schematic illustration of the nanosensor platform and intracellular implementation.

Mentions: Herein, we report an alternative approach that utilizes nanotechnology for cell tracking and status/function assessment without the above concerns. Specifically, a versatile nanoparticle platform (i.e. nanosensors) is developed to efficiently label a wide range of cell types with minimal changes to phenotype11. The nanosensors are prepared by encapsulating sensor molecules within biodegradable polymeric NPs. Thereafter, nanosensors are decorated with moieties to facilitate cell endocytosis. Although specific targeting molecules can be chosen, cationic poly-L-lysine was utilized in this study for efficient endocytosis in vitro. Free nanosensor particles were then separated from labeled cells. Upon intracellular entry, they reside within the cell cytoplasm, serving as a depot to continuously release sensor molecules. These sensors comprise organic fluorophore derivatives (e.g. Calcein Acetomethoxy (CAM)) and hydrophilic oligonucleotide probes designed to detect specific molecular sequences. In the absence of the biomarkers, the released sensor molecules remain ‘Off’. When the biomarker(s) is expressed, a detectable signal is generated (On). (Fig. 1).


A Nanoparticle-based Sensor Platform for Cell Tracking and Status/Function Assessment.

Yeo D, Wiraja C, Chuah YJ, Gao Y, Xu C - Sci Rep (2015)

Schematic illustration of the nanosensor platform and intracellular implementation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4593999&req=5

f1: Schematic illustration of the nanosensor platform and intracellular implementation.
Mentions: Herein, we report an alternative approach that utilizes nanotechnology for cell tracking and status/function assessment without the above concerns. Specifically, a versatile nanoparticle platform (i.e. nanosensors) is developed to efficiently label a wide range of cell types with minimal changes to phenotype11. The nanosensors are prepared by encapsulating sensor molecules within biodegradable polymeric NPs. Thereafter, nanosensors are decorated with moieties to facilitate cell endocytosis. Although specific targeting molecules can be chosen, cationic poly-L-lysine was utilized in this study for efficient endocytosis in vitro. Free nanosensor particles were then separated from labeled cells. Upon intracellular entry, they reside within the cell cytoplasm, serving as a depot to continuously release sensor molecules. These sensors comprise organic fluorophore derivatives (e.g. Calcein Acetomethoxy (CAM)) and hydrophilic oligonucleotide probes designed to detect specific molecular sequences. In the absence of the biomarkers, the released sensor molecules remain ‘Off’. When the biomarker(s) is expressed, a detectable signal is generated (On). (Fig. 1).

Bottom Line: Upon intracellular entry, nanosensors reside within the cell cytoplasm, serving as a depot to continuously release sensor molecules for up to 30 days.When the biomarker(s) is expressed, a detectable signal is generated (On).As a proof-of-concept, three nanosensor formulations were synthesized to monitor cell viability, secretion of nitric oxide, and β-actin mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical &Biomedical Engineering, Nanyang Technological University, Singapore.

ABSTRACT
Nanoparticles are increasingly popular choices for labeling and tracking cells in biomedical applications such as cell therapy. However, all current types of nanoparticles fail to provide real-time, noninvasive monitoring of cell status and functions while often generating false positive signals. Herein, a nanosensor platform to track the real-time expression of specific biomarkers that correlate with cell status and functions is reported. Nanosensors are synthesized by encapsulating various sensor molecules within biodegradable polymeric nanoparticles. Upon intracellular entry, nanosensors reside within the cell cytoplasm, serving as a depot to continuously release sensor molecules for up to 30 days. In the absence of the target biomarkers, the released sensor molecules remain 'Off'. When the biomarker(s) is expressed, a detectable signal is generated (On). As a proof-of-concept, three nanosensor formulations were synthesized to monitor cell viability, secretion of nitric oxide, and β-actin mRNA expression.

No MeSH data available.


Related in: MedlinePlus