Limits...
Gaseous 3-pentanol primes plant immunity against a bacterial speck pathogen, Pseudomonas syringae pv. tomato via salicylic acid and jasmonic acid-dependent signaling pathways in Arabidopsis.

Song GC, Choi HK, Ryu CM - Front Plant Sci (2015)

Bottom Line: We performed quantitative real-time PCR to investigate the 3-pentanol-mediated Arabidopsis immune responses by determining Pathogenesis-Related (PR) gene expression levels associated with defense signaling through salicylic acid (SA), jasmonic acid (JA), and ethylene signaling pathways.Selected Arabidopsis mutants confirmed that the 3-pentanol-mediated immune response involved SA and JA signaling pathways and the NPR1 gene.Taken together, this study indicates that gaseous 3-pentanol triggers induced resistance in Arabidopsis by priming SA and JA signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: Molecular Phytobacteriology Laboratory, Korea Research Institute of Bioscience and Biotechnology , Daejeon, South Korea.

ABSTRACT
3-Pentanol is an active organic compound produced by plants and is a component of emitted insect sex pheromones. A previous study reported that drench application of 3-pentanol elicited plant immunity against microbial pathogens and an insect pest in crop plants. Here, we evaluated whether 3-pentanol and the derivatives 1-pentanol and 2-pentanol induced plant systemic resistance using the in vitro I-plate system. Exposure of Arabidopsis seedlings to 10 μM and 100 nM 3-pentanol evaporate elicited an immune response to Pseudomonas syringae pv. tomato DC3000. We performed quantitative real-time PCR to investigate the 3-pentanol-mediated Arabidopsis immune responses by determining Pathogenesis-Related (PR) gene expression levels associated with defense signaling through salicylic acid (SA), jasmonic acid (JA), and ethylene signaling pathways. The results show that exposure to 3-pentanol and subsequent pathogen challenge upregulated PDF1.2 and PR1 expression. Selected Arabidopsis mutants confirmed that the 3-pentanol-mediated immune response involved SA and JA signaling pathways and the NPR1 gene. Taken together, this study indicates that gaseous 3-pentanol triggers induced resistance in Arabidopsis by priming SA and JA signaling pathways. To our knowledge, this is the first report that a volatile compound of an insect sex pheromone triggers plant systemic resistance against a bacterial pathogen.

No MeSH data available.


Related in: MedlinePlus

Volatile 3-pentanol-mediated induced resistance in Arabidopsis mutants against Pto.Arabidopsis Col-0 seedlings (A), etr1-3(B), jar1-1(C), npr1(D), NahG (E), and sid2(F) were exposed to 100 nM and 10 μM 3-pentanol evaporate or sterile distilled water control. After 5 days, all plants were drop-inoculated with Pto. Disease severity was measured 7 days after pathogen inoculation. Numbers represent means of 12 replications per treatment, with one seedling per replication. Different letters in (A) to (F) indicate significant differences between treatments (P = 0.05 by LSD). Error bars represent means ± SEM. (G) Plants were photographed 7 days after pathogen challenge.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4593957&req=5

Figure 4: Volatile 3-pentanol-mediated induced resistance in Arabidopsis mutants against Pto.Arabidopsis Col-0 seedlings (A), etr1-3(B), jar1-1(C), npr1(D), NahG (E), and sid2(F) were exposed to 100 nM and 10 μM 3-pentanol evaporate or sterile distilled water control. After 5 days, all plants were drop-inoculated with Pto. Disease severity was measured 7 days after pathogen inoculation. Numbers represent means of 12 replications per treatment, with one seedling per replication. Different letters in (A) to (F) indicate significant differences between treatments (P = 0.05 by LSD). Error bars represent means ± SEM. (G) Plants were photographed 7 days after pathogen challenge.

Mentions: Next, we used the I-plate system to test the effects of 100 nM and 10 μM 3-pentanol evaporate on plant defense pathway signaling in five Arabidopsis mutants [npr1 (SA signaling), NahG (SA degradation), sid2 (SA synthesis), jar1-1 (JA-resistant), and etr1-3 (ET receptor mutant)] challenged with Pto DC3000 (Lee et al., 2012). The SA signaling-related mutants npr1, NahG, and sid2, and the JA-resistant mutant jar1-1 displayed severe disease symptoms (Figures 4C–G), whereas Col-0 and etr1-3 consistently displayed induced resistance (Figures 4A,B, G). We obtained similar results from three independent experiments.


Gaseous 3-pentanol primes plant immunity against a bacterial speck pathogen, Pseudomonas syringae pv. tomato via salicylic acid and jasmonic acid-dependent signaling pathways in Arabidopsis.

Song GC, Choi HK, Ryu CM - Front Plant Sci (2015)

Volatile 3-pentanol-mediated induced resistance in Arabidopsis mutants against Pto.Arabidopsis Col-0 seedlings (A), etr1-3(B), jar1-1(C), npr1(D), NahG (E), and sid2(F) were exposed to 100 nM and 10 μM 3-pentanol evaporate or sterile distilled water control. After 5 days, all plants were drop-inoculated with Pto. Disease severity was measured 7 days after pathogen inoculation. Numbers represent means of 12 replications per treatment, with one seedling per replication. Different letters in (A) to (F) indicate significant differences between treatments (P = 0.05 by LSD). Error bars represent means ± SEM. (G) Plants were photographed 7 days after pathogen challenge.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4593957&req=5

Figure 4: Volatile 3-pentanol-mediated induced resistance in Arabidopsis mutants against Pto.Arabidopsis Col-0 seedlings (A), etr1-3(B), jar1-1(C), npr1(D), NahG (E), and sid2(F) were exposed to 100 nM and 10 μM 3-pentanol evaporate or sterile distilled water control. After 5 days, all plants were drop-inoculated with Pto. Disease severity was measured 7 days after pathogen inoculation. Numbers represent means of 12 replications per treatment, with one seedling per replication. Different letters in (A) to (F) indicate significant differences between treatments (P = 0.05 by LSD). Error bars represent means ± SEM. (G) Plants were photographed 7 days after pathogen challenge.
Mentions: Next, we used the I-plate system to test the effects of 100 nM and 10 μM 3-pentanol evaporate on plant defense pathway signaling in five Arabidopsis mutants [npr1 (SA signaling), NahG (SA degradation), sid2 (SA synthesis), jar1-1 (JA-resistant), and etr1-3 (ET receptor mutant)] challenged with Pto DC3000 (Lee et al., 2012). The SA signaling-related mutants npr1, NahG, and sid2, and the JA-resistant mutant jar1-1 displayed severe disease symptoms (Figures 4C–G), whereas Col-0 and etr1-3 consistently displayed induced resistance (Figures 4A,B, G). We obtained similar results from three independent experiments.

Bottom Line: We performed quantitative real-time PCR to investigate the 3-pentanol-mediated Arabidopsis immune responses by determining Pathogenesis-Related (PR) gene expression levels associated with defense signaling through salicylic acid (SA), jasmonic acid (JA), and ethylene signaling pathways.Selected Arabidopsis mutants confirmed that the 3-pentanol-mediated immune response involved SA and JA signaling pathways and the NPR1 gene.Taken together, this study indicates that gaseous 3-pentanol triggers induced resistance in Arabidopsis by priming SA and JA signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: Molecular Phytobacteriology Laboratory, Korea Research Institute of Bioscience and Biotechnology , Daejeon, South Korea.

ABSTRACT
3-Pentanol is an active organic compound produced by plants and is a component of emitted insect sex pheromones. A previous study reported that drench application of 3-pentanol elicited plant immunity against microbial pathogens and an insect pest in crop plants. Here, we evaluated whether 3-pentanol and the derivatives 1-pentanol and 2-pentanol induced plant systemic resistance using the in vitro I-plate system. Exposure of Arabidopsis seedlings to 10 μM and 100 nM 3-pentanol evaporate elicited an immune response to Pseudomonas syringae pv. tomato DC3000. We performed quantitative real-time PCR to investigate the 3-pentanol-mediated Arabidopsis immune responses by determining Pathogenesis-Related (PR) gene expression levels associated with defense signaling through salicylic acid (SA), jasmonic acid (JA), and ethylene signaling pathways. The results show that exposure to 3-pentanol and subsequent pathogen challenge upregulated PDF1.2 and PR1 expression. Selected Arabidopsis mutants confirmed that the 3-pentanol-mediated immune response involved SA and JA signaling pathways and the NPR1 gene. Taken together, this study indicates that gaseous 3-pentanol triggers induced resistance in Arabidopsis by priming SA and JA signaling pathways. To our knowledge, this is the first report that a volatile compound of an insect sex pheromone triggers plant systemic resistance against a bacterial pathogen.

No MeSH data available.


Related in: MedlinePlus