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Molecular Factors Underlying the Deposition of Intramuscular Fat and Collagen in Skeletal Muscle of Nellore and Angus Cattle.

Martins TS, Sanglard LM, Silva W, Chizzotti ML, Rennó LN, Serão NV, Silva FF, Guimarães SE, Ladeira MM, Dodson MV, Du M, Duarte MS - PLoS ONE (2015)

Bottom Line: A greater intramuscular fat content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05).Similarly, no differences were observed in mRNA expression of adipogenic markers Zfp423, PPARγ, and C/EBPα (P>0.05) However, a greater PPARγ protein content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05).These findings demonstrate that difference in intramuscular fat content is associated with a slightly enhanced adipogenesis in skeletal muscle of Angus compared to Nellore cattle, while no difference in fibrogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, Universidade Federal de Viçosa, Viçosa, Brazil.

ABSTRACT
Studies have shown that intramuscular adipogenesis and fibrogenesis may concomitantly occur in skeletal muscle of beef cattle. Thus, we hypothesized that the discrepancy of intramuscular fat content in beef from Nellore and Angus was associated with differences in intramuscular adipogenesis and fibrogenesis during the finishing phase. To test our hypothesis, longissimus muscle samples of Nellore (n = 6; BW = 372.5 ± 37.3 kg) and Angus (n = 6; BW = 382.8 ± 23.9 kg) cattle were collected for analysis of gene and protein expression, and quantification of intramuscular fat and collagen. Least-squares means were estimated for the effect of Breed and differences were considered at P ≤ 0.05. A greater intramuscular fat content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05). No differences were observed for mRNA expression of lipogenic and lipolytic markers ACC, FAS, FABP4, SERBP-1, CPT-2, LPL, and ACOX (P > 0.05) in skeletal muscle of Nellore and Angus cattle. Similarly, no differences were observed in mRNA expression of adipogenic markers Zfp423, PPARγ, and C/EBPα (P>0.05) However, a greater PPARγ protein content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05). A greater abundance of adipo/fibrogenic cells, evaluated by the PDGFRα content, was observed in skeletal muscle of Angus than Nellore cattle (P≤0.05). No differences in fibrogenesis were observed in skeletal muscle of Angus and Nellore cattle, which is in accordance with the lack of differences in intramuscular collagen content in beef from both breeds (P>0.05). These findings demonstrate that difference in intramuscular fat content is associated with a slightly enhanced adipogenesis in skeletal muscle of Angus compared to Nellore cattle, while no difference in fibrogenesis.

No MeSH data available.


Intramuscular fat content and expression of adipogenic markers in skeletal muscle of Angus and Nellore cattle.Intramuscular fat measured by % of ether extract; B) mRNA expression of CCAAT enhancer binding protein, alpha (C/EBPα), Peroxissome proliferator activated-receptor gamma (PPARγ), and Zinc finger protein 423 (Zfp423); C) Zfp423 abundance evaluated by western-blot; D) PPARϒ abundance evaluated by western-blot; Tubulin was used as a loading control. mRNA expression and protein abundance did not differ (P ≥ 0.05).
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pone.0139943.g002: Intramuscular fat content and expression of adipogenic markers in skeletal muscle of Angus and Nellore cattle.Intramuscular fat measured by % of ether extract; B) mRNA expression of CCAAT enhancer binding protein, alpha (C/EBPα), Peroxissome proliferator activated-receptor gamma (PPARγ), and Zinc finger protein 423 (Zfp423); C) Zfp423 abundance evaluated by western-blot; D) PPARϒ abundance evaluated by western-blot; Tubulin was used as a loading control. mRNA expression and protein abundance did not differ (P ≥ 0.05).

Mentions: A greater intramuscular fat content was observed in longissimus muscle of Angus compared to Nellore cattle (P < 0.01; Fig 2A). However, no differences were found for mRNA expression of late adipogenic markers C/EBPα (P = 0.14) and PPARγ (P = 0.42) in longissimus muscle of Angus and Nellore cattle (Fig 2B). Similarly, the mRNA expression of early adipogenic marker Zfp423 in longissimus muscle did not differ (P = 0.62) among breeds (Fig 2B).


Molecular Factors Underlying the Deposition of Intramuscular Fat and Collagen in Skeletal Muscle of Nellore and Angus Cattle.

Martins TS, Sanglard LM, Silva W, Chizzotti ML, Rennó LN, Serão NV, Silva FF, Guimarães SE, Ladeira MM, Dodson MV, Du M, Duarte MS - PLoS ONE (2015)

Intramuscular fat content and expression of adipogenic markers in skeletal muscle of Angus and Nellore cattle.Intramuscular fat measured by % of ether extract; B) mRNA expression of CCAAT enhancer binding protein, alpha (C/EBPα), Peroxissome proliferator activated-receptor gamma (PPARγ), and Zinc finger protein 423 (Zfp423); C) Zfp423 abundance evaluated by western-blot; D) PPARϒ abundance evaluated by western-blot; Tubulin was used as a loading control. mRNA expression and protein abundance did not differ (P ≥ 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4593631&req=5

pone.0139943.g002: Intramuscular fat content and expression of adipogenic markers in skeletal muscle of Angus and Nellore cattle.Intramuscular fat measured by % of ether extract; B) mRNA expression of CCAAT enhancer binding protein, alpha (C/EBPα), Peroxissome proliferator activated-receptor gamma (PPARγ), and Zinc finger protein 423 (Zfp423); C) Zfp423 abundance evaluated by western-blot; D) PPARϒ abundance evaluated by western-blot; Tubulin was used as a loading control. mRNA expression and protein abundance did not differ (P ≥ 0.05).
Mentions: A greater intramuscular fat content was observed in longissimus muscle of Angus compared to Nellore cattle (P < 0.01; Fig 2A). However, no differences were found for mRNA expression of late adipogenic markers C/EBPα (P = 0.14) and PPARγ (P = 0.42) in longissimus muscle of Angus and Nellore cattle (Fig 2B). Similarly, the mRNA expression of early adipogenic marker Zfp423 in longissimus muscle did not differ (P = 0.62) among breeds (Fig 2B).

Bottom Line: A greater intramuscular fat content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05).Similarly, no differences were observed in mRNA expression of adipogenic markers Zfp423, PPARγ, and C/EBPα (P>0.05) However, a greater PPARγ protein content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05).These findings demonstrate that difference in intramuscular fat content is associated with a slightly enhanced adipogenesis in skeletal muscle of Angus compared to Nellore cattle, while no difference in fibrogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, Universidade Federal de Viçosa, Viçosa, Brazil.

ABSTRACT
Studies have shown that intramuscular adipogenesis and fibrogenesis may concomitantly occur in skeletal muscle of beef cattle. Thus, we hypothesized that the discrepancy of intramuscular fat content in beef from Nellore and Angus was associated with differences in intramuscular adipogenesis and fibrogenesis during the finishing phase. To test our hypothesis, longissimus muscle samples of Nellore (n = 6; BW = 372.5 ± 37.3 kg) and Angus (n = 6; BW = 382.8 ± 23.9 kg) cattle were collected for analysis of gene and protein expression, and quantification of intramuscular fat and collagen. Least-squares means were estimated for the effect of Breed and differences were considered at P ≤ 0.05. A greater intramuscular fat content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05). No differences were observed for mRNA expression of lipogenic and lipolytic markers ACC, FAS, FABP4, SERBP-1, CPT-2, LPL, and ACOX (P > 0.05) in skeletal muscle of Nellore and Angus cattle. Similarly, no differences were observed in mRNA expression of adipogenic markers Zfp423, PPARγ, and C/EBPα (P>0.05) However, a greater PPARγ protein content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05). A greater abundance of adipo/fibrogenic cells, evaluated by the PDGFRα content, was observed in skeletal muscle of Angus than Nellore cattle (P≤0.05). No differences in fibrogenesis were observed in skeletal muscle of Angus and Nellore cattle, which is in accordance with the lack of differences in intramuscular collagen content in beef from both breeds (P>0.05). These findings demonstrate that difference in intramuscular fat content is associated with a slightly enhanced adipogenesis in skeletal muscle of Angus compared to Nellore cattle, while no difference in fibrogenesis.

No MeSH data available.