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Effects of Very Low Dose Fast Neutrons on Cell Membrane And Secondary Protein Structure in Rat Erythrocytes.

Saeed A, Raouf GA, Nafee SS, Shaheen SA, Al-Hadeethi Y - PLoS ONE (2015)

Bottom Line: The effects of ionizing radiation on biological cells have been reported in several literatures.The observed band shift in the CH2 stretching bands toward the lower frequencies may be associated with the decrease in membrane fluidity.The curve fitting of the amide I revealed an increase in the percentage area of α-helix opposing a decrease in the β-structure protein secondary structure, which may be attributed to protein denaturation.

View Article: PubMed Central - PubMed

Affiliation: Physics Department, Faculty of Science, King Abdulaziz University, Jeddah, KSA.

ABSTRACT
The effects of ionizing radiation on biological cells have been reported in several literatures. Most of them were mainly concerned with doses greater than 0.01 Gy and were also concerned with gamma rays. On the other hand, the studies on very low dose fast neutrons (VLDFN) are rare. In this study, we have investigated the effects of VLDFN on cell membrane and protein secondary structure of rat erythrocytes. Twelve female Wistar rats were irradiated with neutrons of total dose 0.009 Gy (241Am-Be, 0.2 mGy/h) and twelve others were used as control. Blood samples were taken at the 0, 4th, 8th, and 12th days postirradiation. Fourier transform infrared (FTIR) spectra of rat erythrocytes were recorded. Second derivative and curve fitting were used to analysis FTIR spectra. Hierarchical cluster analysis (HCA) was used to classify group spectra. The second derivative and curve fitting of FTIR spectra revealed that the most significant alterations in the cell membrane and protein secondary structure upon neutron irradiation were detected after 4 days postirradiation. The increase in membrane polarity, phospholipids chain length, packing, and unsaturation were noticed from the corresponding measured FTIR area ratios. This may be due to the membrane lipid peroxidation. The observed band shift in the CH2 stretching bands toward the lower frequencies may be associated with the decrease in membrane fluidity. The curve fitting of the amide I revealed an increase in the percentage area of α-helix opposing a decrease in the β-structure protein secondary structure, which may be attributed to protein denaturation. The results provide detailed insights into the VLDFN effects on erythrocytes. VLDFN can cause an oxidative stress to the irradiated erythrocytes, which appears clearly after 4 days postirradiation.

No MeSH data available.


HCA of rats' erythrocytes Protein and cell membrane FTIR spectra.HCA spectral analysis of protein and carbonyl (C = O) (1800–1500 cm−1); CH3, CH2 asymmetric and symmetric stretching, amid A, and amid B (3500–2800 cm−1) of rat erythrocytes obtained from the control and irradiated groups at 0, 4, 8, and 12 days postirradiation.
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pone.0139854.g006: HCA of rats' erythrocytes Protein and cell membrane FTIR spectra.HCA spectral analysis of protein and carbonyl (C = O) (1800–1500 cm−1); CH3, CH2 asymmetric and symmetric stretching, amid A, and amid B (3500–2800 cm−1) of rat erythrocytes obtained from the control and irradiated groups at 0, 4, 8, and 12 days postirradiation.

Mentions: The dendrograms of HCA created two initial clusters represented by two branches, which were further subdivided into smaller clusters (Fig 6). Wards method Euclidean distances dendrogram, linkage tree, succeeded to distinguish between irradiated and control rats at 4 days postirradiation. However, it failed to distinguish between irradiated and control rats in other groups.


Effects of Very Low Dose Fast Neutrons on Cell Membrane And Secondary Protein Structure in Rat Erythrocytes.

Saeed A, Raouf GA, Nafee SS, Shaheen SA, Al-Hadeethi Y - PLoS ONE (2015)

HCA of rats' erythrocytes Protein and cell membrane FTIR spectra.HCA spectral analysis of protein and carbonyl (C = O) (1800–1500 cm−1); CH3, CH2 asymmetric and symmetric stretching, amid A, and amid B (3500–2800 cm−1) of rat erythrocytes obtained from the control and irradiated groups at 0, 4, 8, and 12 days postirradiation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4593584&req=5

pone.0139854.g006: HCA of rats' erythrocytes Protein and cell membrane FTIR spectra.HCA spectral analysis of protein and carbonyl (C = O) (1800–1500 cm−1); CH3, CH2 asymmetric and symmetric stretching, amid A, and amid B (3500–2800 cm−1) of rat erythrocytes obtained from the control and irradiated groups at 0, 4, 8, and 12 days postirradiation.
Mentions: The dendrograms of HCA created two initial clusters represented by two branches, which were further subdivided into smaller clusters (Fig 6). Wards method Euclidean distances dendrogram, linkage tree, succeeded to distinguish between irradiated and control rats at 4 days postirradiation. However, it failed to distinguish between irradiated and control rats in other groups.

Bottom Line: The effects of ionizing radiation on biological cells have been reported in several literatures.The observed band shift in the CH2 stretching bands toward the lower frequencies may be associated with the decrease in membrane fluidity.The curve fitting of the amide I revealed an increase in the percentage area of α-helix opposing a decrease in the β-structure protein secondary structure, which may be attributed to protein denaturation.

View Article: PubMed Central - PubMed

Affiliation: Physics Department, Faculty of Science, King Abdulaziz University, Jeddah, KSA.

ABSTRACT
The effects of ionizing radiation on biological cells have been reported in several literatures. Most of them were mainly concerned with doses greater than 0.01 Gy and were also concerned with gamma rays. On the other hand, the studies on very low dose fast neutrons (VLDFN) are rare. In this study, we have investigated the effects of VLDFN on cell membrane and protein secondary structure of rat erythrocytes. Twelve female Wistar rats were irradiated with neutrons of total dose 0.009 Gy (241Am-Be, 0.2 mGy/h) and twelve others were used as control. Blood samples were taken at the 0, 4th, 8th, and 12th days postirradiation. Fourier transform infrared (FTIR) spectra of rat erythrocytes were recorded. Second derivative and curve fitting were used to analysis FTIR spectra. Hierarchical cluster analysis (HCA) was used to classify group spectra. The second derivative and curve fitting of FTIR spectra revealed that the most significant alterations in the cell membrane and protein secondary structure upon neutron irradiation were detected after 4 days postirradiation. The increase in membrane polarity, phospholipids chain length, packing, and unsaturation were noticed from the corresponding measured FTIR area ratios. This may be due to the membrane lipid peroxidation. The observed band shift in the CH2 stretching bands toward the lower frequencies may be associated with the decrease in membrane fluidity. The curve fitting of the amide I revealed an increase in the percentage area of α-helix opposing a decrease in the β-structure protein secondary structure, which may be attributed to protein denaturation. The results provide detailed insights into the VLDFN effects on erythrocytes. VLDFN can cause an oxidative stress to the irradiated erythrocytes, which appears clearly after 4 days postirradiation.

No MeSH data available.