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Construction of a Genetic Linkage Map and Identification of QTLs for Seed Weight and Seed Size Traits in Lentil (Lens culinaris Medik.).

Verma P, Goyal R, Chahota RK, Sharma TR, Abdin MZ, Bhatia S - PLoS ONE (2015)

Bottom Line: Six hundred forty seven SSR markers (including 146 previously published) were screened for parental polymorphism and 219 (33.8%) were found to be polymorphic among the parents.Phenotypic data from the RILs was used to identify QTLs for the seed weight and seed size traits by single marker analysis (SMA) followed by composite interval mapping (CIM) which resulted in one QTL each for the 2 traits (qSW and qSS) that were co-localized on LG4 and explained 48.4% and 27.5% of phenotypic variance respectively.The current study would serve as a strong foundation for further validation and fine mapping for utilization in lentil breeding programs.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Plant Genome Research, Post Box No. 10531, Aruna Asaf Ali Marg, New Delhi, 110067, India; Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi, 110062, India.

ABSTRACT
Seed weight and seed size both are quantitative traits and have been considered as important components of grain yield, thus identification of quantitative trait loci (QTL) for seed traits in lentil (Lens culinaris) would be beneficial for the improvement of grain yield. Hence the main objective of this study was to identify QTLs for seed traits using an intraspecific mapping population derived from a cross between L. culinaris cv. Precoz (seed weight-5.1g, seed size-5.7mm) and L. culinaris cv. L830 (seed weight-2.2g, seed size-4mm) comprising 126 F8-RILs. For this, two microsatellite genomic libraries enriched for (GA/CT) and (GAA/CTT) motif were constructed which resulted in the development of 501 new genomic SSR markers. Six hundred forty seven SSR markers (including 146 previously published) were screened for parental polymorphism and 219 (33.8%) were found to be polymorphic among the parents. Of these 216 were mapped on seven linkage groups at LOD4.0 spanning 1183.7cM with an average marker density of 5.48cM. Phenotypic data from the RILs was used to identify QTLs for the seed weight and seed size traits by single marker analysis (SMA) followed by composite interval mapping (CIM) which resulted in one QTL each for the 2 traits (qSW and qSS) that were co-localized on LG4 and explained 48.4% and 27.5% of phenotypic variance respectively. The current study would serve as a strong foundation for further validation and fine mapping for utilization in lentil breeding programs.

No MeSH data available.


Related in: MedlinePlus

An intraspecific linkage map of L. culinaris based on RIL mapping population generated by crossing L. culinaris cv. Precoz× L. culinaris cv. L830.The map was generated with 219 polymorphic genomic SSR markers using JoinMap version 4.0 at a LOD value of 4.0, with Kosambi mapping function. A total of 216 markers were mapped on seven linkage groups (LGs) (LG1 to LG7). The previously mapped markers used in this study are underlined. The distorted markers have been marked with “*” on the right side of the marker name.
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pone.0139666.g001: An intraspecific linkage map of L. culinaris based on RIL mapping population generated by crossing L. culinaris cv. Precoz× L. culinaris cv. L830.The map was generated with 219 polymorphic genomic SSR markers using JoinMap version 4.0 at a LOD value of 4.0, with Kosambi mapping function. A total of 216 markers were mapped on seven linkage groups (LGs) (LG1 to LG7). The previously mapped markers used in this study are underlined. The distorted markers have been marked with “*” on the right side of the marker name.

Mentions: An intraspecific genetic linkage map of lentil was constructed using the genotyping data (S2 Table) of 219 polymorphic markers using the JoinMap software. At LOD 4.0, 216 markers among 219 polymorphic markers were assigned positions on seven linkage groups (LGs). The intra-specific map generated here spanned 1183.7 cM distance of the lentil genome with an average marker density of 5.48 cM (Fig 1). The 216 mapped markers included 174 LcSSR series (generated in this study and published earlier), 7 markers of SSR series and 11 markers of GLLC series. Each linkage group differed from each other with respect to total number of markers mapped, total cM distance and marker density (Table 2). A large variation in length was exhibited by these seven linkage groups which varied from a minimum of 78.8 cM (LG7) to a maximum length of 229.1 cM (LG6). The average marker density on each linkage group revealed that the markers were distributed randomly and unevenly (Fig 1). The marker density was highest in LG3 (3.6cM) which harbored 47 markers, and lowest in LG6 (10.91cM) with 21 markers.


Construction of a Genetic Linkage Map and Identification of QTLs for Seed Weight and Seed Size Traits in Lentil (Lens culinaris Medik.).

Verma P, Goyal R, Chahota RK, Sharma TR, Abdin MZ, Bhatia S - PLoS ONE (2015)

An intraspecific linkage map of L. culinaris based on RIL mapping population generated by crossing L. culinaris cv. Precoz× L. culinaris cv. L830.The map was generated with 219 polymorphic genomic SSR markers using JoinMap version 4.0 at a LOD value of 4.0, with Kosambi mapping function. A total of 216 markers were mapped on seven linkage groups (LGs) (LG1 to LG7). The previously mapped markers used in this study are underlined. The distorted markers have been marked with “*” on the right side of the marker name.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4593543&req=5

pone.0139666.g001: An intraspecific linkage map of L. culinaris based on RIL mapping population generated by crossing L. culinaris cv. Precoz× L. culinaris cv. L830.The map was generated with 219 polymorphic genomic SSR markers using JoinMap version 4.0 at a LOD value of 4.0, with Kosambi mapping function. A total of 216 markers were mapped on seven linkage groups (LGs) (LG1 to LG7). The previously mapped markers used in this study are underlined. The distorted markers have been marked with “*” on the right side of the marker name.
Mentions: An intraspecific genetic linkage map of lentil was constructed using the genotyping data (S2 Table) of 219 polymorphic markers using the JoinMap software. At LOD 4.0, 216 markers among 219 polymorphic markers were assigned positions on seven linkage groups (LGs). The intra-specific map generated here spanned 1183.7 cM distance of the lentil genome with an average marker density of 5.48 cM (Fig 1). The 216 mapped markers included 174 LcSSR series (generated in this study and published earlier), 7 markers of SSR series and 11 markers of GLLC series. Each linkage group differed from each other with respect to total number of markers mapped, total cM distance and marker density (Table 2). A large variation in length was exhibited by these seven linkage groups which varied from a minimum of 78.8 cM (LG7) to a maximum length of 229.1 cM (LG6). The average marker density on each linkage group revealed that the markers were distributed randomly and unevenly (Fig 1). The marker density was highest in LG3 (3.6cM) which harbored 47 markers, and lowest in LG6 (10.91cM) with 21 markers.

Bottom Line: Six hundred forty seven SSR markers (including 146 previously published) were screened for parental polymorphism and 219 (33.8%) were found to be polymorphic among the parents.Phenotypic data from the RILs was used to identify QTLs for the seed weight and seed size traits by single marker analysis (SMA) followed by composite interval mapping (CIM) which resulted in one QTL each for the 2 traits (qSW and qSS) that were co-localized on LG4 and explained 48.4% and 27.5% of phenotypic variance respectively.The current study would serve as a strong foundation for further validation and fine mapping for utilization in lentil breeding programs.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Plant Genome Research, Post Box No. 10531, Aruna Asaf Ali Marg, New Delhi, 110067, India; Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi, 110062, India.

ABSTRACT
Seed weight and seed size both are quantitative traits and have been considered as important components of grain yield, thus identification of quantitative trait loci (QTL) for seed traits in lentil (Lens culinaris) would be beneficial for the improvement of grain yield. Hence the main objective of this study was to identify QTLs for seed traits using an intraspecific mapping population derived from a cross between L. culinaris cv. Precoz (seed weight-5.1g, seed size-5.7mm) and L. culinaris cv. L830 (seed weight-2.2g, seed size-4mm) comprising 126 F8-RILs. For this, two microsatellite genomic libraries enriched for (GA/CT) and (GAA/CTT) motif were constructed which resulted in the development of 501 new genomic SSR markers. Six hundred forty seven SSR markers (including 146 previously published) were screened for parental polymorphism and 219 (33.8%) were found to be polymorphic among the parents. Of these 216 were mapped on seven linkage groups at LOD4.0 spanning 1183.7cM with an average marker density of 5.48cM. Phenotypic data from the RILs was used to identify QTLs for the seed weight and seed size traits by single marker analysis (SMA) followed by composite interval mapping (CIM) which resulted in one QTL each for the 2 traits (qSW and qSS) that were co-localized on LG4 and explained 48.4% and 27.5% of phenotypic variance respectively. The current study would serve as a strong foundation for further validation and fine mapping for utilization in lentil breeding programs.

No MeSH data available.


Related in: MedlinePlus