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Novel Paramyxoviruses in Bats from Sub-Saharan Africa, 2007-2012.

Mortlock M, Kuzmin IV, Weyer J, Gilbert AT, Agwanda B, Rupprecht CE, Nel LH, Kearney T, Malekani JM, Markotter W - Emerging Infect. Dis. (2015)

Bottom Line: As part of a larger survey for detection of pathogens among wildlife in sub-Saharan Africa conducted during 2007-2012, multiple diverse paramyxovirus sequences were detected in renal tissues of bats.Phylogenetic analysis supports the presence of at least 2 major viral lineages and suggests that paramyxoviruses are strongly associated with several bat genera.

View Article: PubMed Central - PubMed

ABSTRACT
As part of a larger survey for detection of pathogens among wildlife in sub-Saharan Africa conducted during 2007-2012, multiple diverse paramyxovirus sequences were detected in renal tissues of bats. Phylogenetic analysis supports the presence of at least 2 major viral lineages and suggests that paramyxoviruses are strongly associated with several bat genera.

No MeSH data available.


Related in: MedlinePlus

Maximum clade credibility tree based on partial polymerase (large) gene sequences (439 bp) of paramyxoviruses built in BEAST version 1.7.4 software (http://beast.bio.ed.ac.uk/), applying the general time reversible plus invariant sites plus gamma model inferred by jModelTest version 0.1.1 (10). Sequences detected in this study are indicated in red. Identical sequences were collapsed to only show a representative. Genus-specific clusters are indicated on the right and show possible opportunistic infections in other species grouping within these clusters. The 2 lineages reported are indicated in the gray areas.
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Figure 1: Maximum clade credibility tree based on partial polymerase (large) gene sequences (439 bp) of paramyxoviruses built in BEAST version 1.7.4 software (http://beast.bio.ed.ac.uk/), applying the general time reversible plus invariant sites plus gamma model inferred by jModelTest version 0.1.1 (10). Sequences detected in this study are indicated in red. Identical sequences were collapsed to only show a representative. Genus-specific clusters are indicated on the right and show possible opportunistic infections in other species grouping within these clusters. The 2 lineages reported are indicated in the gray areas.

Mentions: During 2007–2012, we sampled 1,220 bats representing at least 48 species from multiple locations in selected countries in Africa (Table 1). Bats were anesthetized with the use of ketamine (0.05–0.1 mg/g body mass) and exsanguinated by cardiac puncture. Voucher specimens were identified through morphologic characterization (7) or, alternatively, through genetic barcoding. Approximately 30–100 mg of renal tissue was used for RNA extraction. A heminested primer set targeting the conserved polymerase (large) gene of Respirovirus, Morbillivirus, and Henipavirus was used for sample screening through reverse transcription PCR (8). A total of 103 samples (8.4%) tested positive, and the obtained amplicons of ≈490 bp were sequenced (Technical Appendix Table 1). For phylogenetic analysis, representative paramyxovirus sequences available from GenBank were included (Technical Appendix Table 2), and Bayesian analysis was performed by using BEAST version 1.7.4 software (http://beast.bio.ed.ac.uk/) (Figure).


Novel Paramyxoviruses in Bats from Sub-Saharan Africa, 2007-2012.

Mortlock M, Kuzmin IV, Weyer J, Gilbert AT, Agwanda B, Rupprecht CE, Nel LH, Kearney T, Malekani JM, Markotter W - Emerging Infect. Dis. (2015)

Maximum clade credibility tree based on partial polymerase (large) gene sequences (439 bp) of paramyxoviruses built in BEAST version 1.7.4 software (http://beast.bio.ed.ac.uk/), applying the general time reversible plus invariant sites plus gamma model inferred by jModelTest version 0.1.1 (10). Sequences detected in this study are indicated in red. Identical sequences were collapsed to only show a representative. Genus-specific clusters are indicated on the right and show possible opportunistic infections in other species grouping within these clusters. The 2 lineages reported are indicated in the gray areas.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4593419&req=5

Figure 1: Maximum clade credibility tree based on partial polymerase (large) gene sequences (439 bp) of paramyxoviruses built in BEAST version 1.7.4 software (http://beast.bio.ed.ac.uk/), applying the general time reversible plus invariant sites plus gamma model inferred by jModelTest version 0.1.1 (10). Sequences detected in this study are indicated in red. Identical sequences were collapsed to only show a representative. Genus-specific clusters are indicated on the right and show possible opportunistic infections in other species grouping within these clusters. The 2 lineages reported are indicated in the gray areas.
Mentions: During 2007–2012, we sampled 1,220 bats representing at least 48 species from multiple locations in selected countries in Africa (Table 1). Bats were anesthetized with the use of ketamine (0.05–0.1 mg/g body mass) and exsanguinated by cardiac puncture. Voucher specimens were identified through morphologic characterization (7) or, alternatively, through genetic barcoding. Approximately 30–100 mg of renal tissue was used for RNA extraction. A heminested primer set targeting the conserved polymerase (large) gene of Respirovirus, Morbillivirus, and Henipavirus was used for sample screening through reverse transcription PCR (8). A total of 103 samples (8.4%) tested positive, and the obtained amplicons of ≈490 bp were sequenced (Technical Appendix Table 1). For phylogenetic analysis, representative paramyxovirus sequences available from GenBank were included (Technical Appendix Table 2), and Bayesian analysis was performed by using BEAST version 1.7.4 software (http://beast.bio.ed.ac.uk/) (Figure).

Bottom Line: As part of a larger survey for detection of pathogens among wildlife in sub-Saharan Africa conducted during 2007-2012, multiple diverse paramyxovirus sequences were detected in renal tissues of bats.Phylogenetic analysis supports the presence of at least 2 major viral lineages and suggests that paramyxoviruses are strongly associated with several bat genera.

View Article: PubMed Central - PubMed

ABSTRACT
As part of a larger survey for detection of pathogens among wildlife in sub-Saharan Africa conducted during 2007-2012, multiple diverse paramyxovirus sequences were detected in renal tissues of bats. Phylogenetic analysis supports the presence of at least 2 major viral lineages and suggests that paramyxoviruses are strongly associated with several bat genera.

No MeSH data available.


Related in: MedlinePlus