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Association of insulin-like growth factor-binding protein-3 with radiotherapy response and prognosis of esophageal squamous cell carcinoma.

Luo LL, Zhao L, Xi M, He LR, Shen JX, Li QQ, Liu SL, Zhang P, Xie D, Liu MZ - Chin J Cancer (2015)

Bottom Line: The threshold for IGFBP-3 positivity was set to greater than 65% [area under the ROC curve (AUC) = 0.690, P < 0.019].The levels of IGFBP-3 protein expression were decreased in 70.0% (7 of 10) of ESCC tissues compared with adjacent non-malignant esophageal tissue.In addition, IGFBP-3 expression was associated with pathologic classification (P < 0.05 for T, N, and M categories and clinical stage).

View Article: PubMed Central - PubMed

Affiliation: Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, Guangdong, P.R. China. luolil@sysucc.org.cn.

ABSTRACT

Background: Insulin-like growth factor-binding protein-3 (IGFBP-3) is suggested to predict the radiosensitivity and/or prognosis of patients with esophageal squamous cell carcinoma (ESCC). The present study was designed to investigate the clinical and prognostic effects of IGFBP-3 on ESCC.

Methods: IGFBP-3 was detected by immunohistochemistry in paraffin-embedded tissues from 70 ESCC patients treated with radiotherapy alone and further examined by western blotting analysis in 10 pairs of fresh ESCC tissues and adjacent non-malignant esophageal specimens. Receiver operating characteristic (ROC) analysis was used to determine cut-off scores for tumor positivity and to evaluate patient survival status. The χ(2) test was performed to analyze the association of IGFBP-3 expression with clinical characteristics and radiotherapy response. Associations between prognostic outcomes and IGFBP-3 expression were investigated using Kaplan-Meier analysis and the Cox proportional hazards model.

Results: The threshold for IGFBP-3 positivity was set to greater than 65% [area under the ROC curve (AUC) = 0.690, P < 0.019]. Of the 70 ESCC patient tissues tested, 32 (45.7%) were defined as having high IGFBP-3 expression. The levels of IGFBP-3 protein expression were decreased in 70.0% (7 of 10) of ESCC tissues compared with adjacent non-malignant esophageal tissue. In addition, IGFBP-3 expression was associated with pathologic classification (P < 0.05 for T, N, and M categories and clinical stage). Patients with elevated protein level of IGFBP-3 in the tumor had an improved radiotherapy response and prolonged overall survival (P < 0.001).

Conclusions: High level of IGFBP-3 expression in ESCC associates with early clinical stages and are predictive for favorable survival of the patients treated with radiotherapy.

No MeSH data available.


Related in: MedlinePlus

Receiver operating characteristic (ROC) analysis was performed to determine the cut-off score for the positive expression of insulin-like growth factor-binding protein-3 (IGFBP-3) in 70 esophageal squamous cell carcinoma (ESCC) patients. The sensitivity and specificity for each outcome were plotted: a T category, b N category, c M category, d tumor grade, e survival status, and f progression-free survival
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Fig1: Receiver operating characteristic (ROC) analysis was performed to determine the cut-off score for the positive expression of insulin-like growth factor-binding protein-3 (IGFBP-3) in 70 esophageal squamous cell carcinoma (ESCC) patients. The sensitivity and specificity for each outcome were plotted: a T category, b N category, c M category, d tumor grade, e survival status, and f progression-free survival

Mentions: The ROC analysis for each clinicopathologic parameter showed the point on the curve closest to (0.0, 1.0), which maximizes both the sensitivity and specificity for the outcome (Fig. 1). The corresponding areas under the ROC curve (AUC) with 95% confidence interval (CI) are shown in Table 2. According to the ROC analysis, values above the critical value of 0.65 were defined as positive for IGFBP-3 protein expression.Fig. 1


Association of insulin-like growth factor-binding protein-3 with radiotherapy response and prognosis of esophageal squamous cell carcinoma.

Luo LL, Zhao L, Xi M, He LR, Shen JX, Li QQ, Liu SL, Zhang P, Xie D, Liu MZ - Chin J Cancer (2015)

Receiver operating characteristic (ROC) analysis was performed to determine the cut-off score for the positive expression of insulin-like growth factor-binding protein-3 (IGFBP-3) in 70 esophageal squamous cell carcinoma (ESCC) patients. The sensitivity and specificity for each outcome were plotted: a T category, b N category, c M category, d tumor grade, e survival status, and f progression-free survival
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4593339&req=5

Fig1: Receiver operating characteristic (ROC) analysis was performed to determine the cut-off score for the positive expression of insulin-like growth factor-binding protein-3 (IGFBP-3) in 70 esophageal squamous cell carcinoma (ESCC) patients. The sensitivity and specificity for each outcome were plotted: a T category, b N category, c M category, d tumor grade, e survival status, and f progression-free survival
Mentions: The ROC analysis for each clinicopathologic parameter showed the point on the curve closest to (0.0, 1.0), which maximizes both the sensitivity and specificity for the outcome (Fig. 1). The corresponding areas under the ROC curve (AUC) with 95% confidence interval (CI) are shown in Table 2. According to the ROC analysis, values above the critical value of 0.65 were defined as positive for IGFBP-3 protein expression.Fig. 1

Bottom Line: The threshold for IGFBP-3 positivity was set to greater than 65% [area under the ROC curve (AUC) = 0.690, P < 0.019].The levels of IGFBP-3 protein expression were decreased in 70.0% (7 of 10) of ESCC tissues compared with adjacent non-malignant esophageal tissue.In addition, IGFBP-3 expression was associated with pathologic classification (P < 0.05 for T, N, and M categories and clinical stage).

View Article: PubMed Central - PubMed

Affiliation: Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, Guangdong, P.R. China. luolil@sysucc.org.cn.

ABSTRACT

Background: Insulin-like growth factor-binding protein-3 (IGFBP-3) is suggested to predict the radiosensitivity and/or prognosis of patients with esophageal squamous cell carcinoma (ESCC). The present study was designed to investigate the clinical and prognostic effects of IGFBP-3 on ESCC.

Methods: IGFBP-3 was detected by immunohistochemistry in paraffin-embedded tissues from 70 ESCC patients treated with radiotherapy alone and further examined by western blotting analysis in 10 pairs of fresh ESCC tissues and adjacent non-malignant esophageal specimens. Receiver operating characteristic (ROC) analysis was used to determine cut-off scores for tumor positivity and to evaluate patient survival status. The χ(2) test was performed to analyze the association of IGFBP-3 expression with clinical characteristics and radiotherapy response. Associations between prognostic outcomes and IGFBP-3 expression were investigated using Kaplan-Meier analysis and the Cox proportional hazards model.

Results: The threshold for IGFBP-3 positivity was set to greater than 65% [area under the ROC curve (AUC) = 0.690, P < 0.019]. Of the 70 ESCC patient tissues tested, 32 (45.7%) were defined as having high IGFBP-3 expression. The levels of IGFBP-3 protein expression were decreased in 70.0% (7 of 10) of ESCC tissues compared with adjacent non-malignant esophageal tissue. In addition, IGFBP-3 expression was associated with pathologic classification (P < 0.05 for T, N, and M categories and clinical stage). Patients with elevated protein level of IGFBP-3 in the tumor had an improved radiotherapy response and prolonged overall survival (P < 0.001).

Conclusions: High level of IGFBP-3 expression in ESCC associates with early clinical stages and are predictive for favorable survival of the patients treated with radiotherapy.

No MeSH data available.


Related in: MedlinePlus