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Protective Role of Crocin Against Nicotine-induced Damages on Male Mice Liver.

Jalili C, Tabatabaei H, Kakaberiei S, Roshankhah S, Salahshoor MR - Int J Prev Med (2015)

Bottom Line: It has different pharmacological effects such as antioxidant and anticancer.The results indicated that nicotine administration significantly decreased liver weight (48.37%) and increased the mean diameter of hepatocyte (239%), central hepatic vein (28.45%), liver enzymes level (ALP 29.43%, AST 21.81%, ALT 21.55%), and blood serum nitric oxide level (57.18%) compared to saline group (P < 0.05).However, crocin and crocin plus nicotine administration significantly boosted liver weight (49.54%) and decreased the mean diameter of hepatocyte (40.48%), central hepatic vein (15.44%), liver enzymes (ALP 22.02%, AST 19.05%, ALT 23.11%), and nitric oxide levels (35.80%) in all groups compared to nicotine group (percentages represent the maximum dose) (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

ABSTRACT

Background: Nicotine is a major pharmacologically active substance in cigarette smoke. It is mainly metabolized in liver and causes devastating effects. Crocin is the chemical ingredient primarily responsible for the color of saffron. It has different pharmacological effects such as antioxidant and anticancer. This study was designed to evaluate the protective role of crocin against nicotine on the liver of mice.

Methods: Forty-eight mice were equally divided into 8 groups; control (normal saline), nicotine (2.5 mg/kg), crocin (12.5, 25 and 50 mg/kg) and crocin plus nicotine treated groups. Saline, crocin, nicotine and crocin/nicotine (once a day) were intraperitoneally injected for 4 weeks. The liver weight and histology, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and serum nitric oxide levels have been studied.

Results: The results indicated that nicotine administration significantly decreased liver weight (48.37%) and increased the mean diameter of hepatocyte (239%), central hepatic vein (28.45%), liver enzymes level (ALP 29.43%, AST 21.81%, ALT 21.55%), and blood serum nitric oxide level (57.18%) compared to saline group (P < 0.05). However, crocin and crocin plus nicotine administration significantly boosted liver weight (49.54%) and decreased the mean diameter of hepatocyte (40.48%), central hepatic vein (15.44%), liver enzymes (ALP 22.02%, AST 19.05%, ALT 23.11%), and nitric oxide levels (35.80%) in all groups compared to nicotine group (percentages represent the maximum dose) (P < 0.05).

Conclusions: Crocin showed its partly protective effect against nicotine-induced liver toxicity.

No MeSH data available.


Related in: MedlinePlus

Forty-eight mice were equally divided into 8 groups. *Significant increase of alanine aminotransferase enzyme in nicotine group compared to saline group (P < 0.05). **Significant decrease in all groups of crocin compared to nicotine group administration (P < 0.05). ***Significant decrease in all groups of crocin-nicotine compared to nicotine group (P < 0.05). The numbers above the columns of the chart are mean ± standard error of triplicate experiments
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Figure 5: Forty-eight mice were equally divided into 8 groups. *Significant increase of alanine aminotransferase enzyme in nicotine group compared to saline group (P < 0.05). **Significant decrease in all groups of crocin compared to nicotine group administration (P < 0.05). ***Significant decrease in all groups of crocin-nicotine compared to nicotine group (P < 0.05). The numbers above the columns of the chart are mean ± standard error of triplicate experiments

Mentions: Nicotine (2.5 ml/kg) caused a significant increase in the mean of ALT, AST, and ALP enzymes compared to saline group (P < 0.05). In addition, the mean of ALT, AST, and ALP enzymes decreased significantly in crocin and crocin-nicotine in all groups administration compared to nicotine group (P < 0.05) [Figures 5-7].


Protective Role of Crocin Against Nicotine-induced Damages on Male Mice Liver.

Jalili C, Tabatabaei H, Kakaberiei S, Roshankhah S, Salahshoor MR - Int J Prev Med (2015)

Forty-eight mice were equally divided into 8 groups. *Significant increase of alanine aminotransferase enzyme in nicotine group compared to saline group (P < 0.05). **Significant decrease in all groups of crocin compared to nicotine group administration (P < 0.05). ***Significant decrease in all groups of crocin-nicotine compared to nicotine group (P < 0.05). The numbers above the columns of the chart are mean ± standard error of triplicate experiments
© Copyright Policy - open-access
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC4593238&req=5

Figure 5: Forty-eight mice were equally divided into 8 groups. *Significant increase of alanine aminotransferase enzyme in nicotine group compared to saline group (P < 0.05). **Significant decrease in all groups of crocin compared to nicotine group administration (P < 0.05). ***Significant decrease in all groups of crocin-nicotine compared to nicotine group (P < 0.05). The numbers above the columns of the chart are mean ± standard error of triplicate experiments
Mentions: Nicotine (2.5 ml/kg) caused a significant increase in the mean of ALT, AST, and ALP enzymes compared to saline group (P < 0.05). In addition, the mean of ALT, AST, and ALP enzymes decreased significantly in crocin and crocin-nicotine in all groups administration compared to nicotine group (P < 0.05) [Figures 5-7].

Bottom Line: It has different pharmacological effects such as antioxidant and anticancer.The results indicated that nicotine administration significantly decreased liver weight (48.37%) and increased the mean diameter of hepatocyte (239%), central hepatic vein (28.45%), liver enzymes level (ALP 29.43%, AST 21.81%, ALT 21.55%), and blood serum nitric oxide level (57.18%) compared to saline group (P < 0.05).However, crocin and crocin plus nicotine administration significantly boosted liver weight (49.54%) and decreased the mean diameter of hepatocyte (40.48%), central hepatic vein (15.44%), liver enzymes (ALP 22.02%, AST 19.05%, ALT 23.11%), and nitric oxide levels (35.80%) in all groups compared to nicotine group (percentages represent the maximum dose) (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

ABSTRACT

Background: Nicotine is a major pharmacologically active substance in cigarette smoke. It is mainly metabolized in liver and causes devastating effects. Crocin is the chemical ingredient primarily responsible for the color of saffron. It has different pharmacological effects such as antioxidant and anticancer. This study was designed to evaluate the protective role of crocin against nicotine on the liver of mice.

Methods: Forty-eight mice were equally divided into 8 groups; control (normal saline), nicotine (2.5 mg/kg), crocin (12.5, 25 and 50 mg/kg) and crocin plus nicotine treated groups. Saline, crocin, nicotine and crocin/nicotine (once a day) were intraperitoneally injected for 4 weeks. The liver weight and histology, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and serum nitric oxide levels have been studied.

Results: The results indicated that nicotine administration significantly decreased liver weight (48.37%) and increased the mean diameter of hepatocyte (239%), central hepatic vein (28.45%), liver enzymes level (ALP 29.43%, AST 21.81%, ALT 21.55%), and blood serum nitric oxide level (57.18%) compared to saline group (P < 0.05). However, crocin and crocin plus nicotine administration significantly boosted liver weight (49.54%) and decreased the mean diameter of hepatocyte (40.48%), central hepatic vein (15.44%), liver enzymes (ALP 22.02%, AST 19.05%, ALT 23.11%), and nitric oxide levels (35.80%) in all groups compared to nicotine group (percentages represent the maximum dose) (P < 0.05).

Conclusions: Crocin showed its partly protective effect against nicotine-induced liver toxicity.

No MeSH data available.


Related in: MedlinePlus