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p38 MAPK regulates PKAα and CUB-serine protease in Amphibalanus amphitrite cyprids.

Zhang G, He LS, Him Wong Y, Xu Y, Zhang Y, Qian PY - Sci Rep (2015)

Bottom Line: The results showed that the expression levels of 80 proteins were significantly modified (p < 0.05).These differentially expressed proteins were assigned to 15 functional groups according to the KOG database and 9 pathways were significantly enriched.Overall, p38 MAPK might regulate multiple functions in A. amphitrite cyprids, including the energy supply, metamorphosis, neural system and cement glands.

View Article: PubMed Central - PubMed

Affiliation: Environmental Science Programs and Division of Life Science, School of Science, the Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, P. R. China.

ABSTRACT
The MKK3-p38 MAPK pathway has been reported to mediate larval settlement in Amphibalanus (=Balanus) amphitrite. To clarify the underlying molecular mechanism, we applied label-free proteomics to analyze changes in the proteome of cyprids treated with a p38 MAPK inhibitor. The results showed that the expression levels of 80 proteins were significantly modified (p < 0.05). These differentially expressed proteins were assigned to 15 functional groups according to the KOG database and 9 pathways were significantly enriched. Further analysis revealed that p38 MAPK might regulate the energy supply and metamorphosis. Two potential regulatory proteins, CUB-serine protease and PKAα, were both down-regulated in expression. CUB-serine protease localized to postaxial seta 2 and 3, as well as the 4 subterminal sensilla in the antennule. Importantly, it was co-localized with the neuron transmitter serotonin in the sections, suggesting that the CUB-serine protease was present in the neural system. PKAα was highly expressed during the cyprid and juvenile stages, and it was co-localized with phospho-p38 MAPK (pp38 MAPK) to the cement gland, suggesting that PKAα might have some functions in cement glands. Overall, p38 MAPK might regulate multiple functions in A. amphitrite cyprids, including the energy supply, metamorphosis, neural system and cement glands.

No MeSH data available.


Characterization of PKAα expression.(A) Western blot showing that the level of PKAα decreased in response to 10 and 20 μM SB203580. (B) PKAα was highly expressed during the cyprid and juvenile stages.
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f6: Characterization of PKAα expression.(A) Western blot showing that the level of PKAα decreased in response to 10 and 20 μM SB203580. (B) PKAα was highly expressed during the cyprid and juvenile stages.

Mentions: Western blot analysis confirmed that the inhibition of p38 MAPK using SB203580 decreased the level of PKAα (Fig. 6A). Among the developmental stages, PKAα was highly expressed in the cyprid and juvenile stages (Fig. 6B).


p38 MAPK regulates PKAα and CUB-serine protease in Amphibalanus amphitrite cyprids.

Zhang G, He LS, Him Wong Y, Xu Y, Zhang Y, Qian PY - Sci Rep (2015)

Characterization of PKAα expression.(A) Western blot showing that the level of PKAα decreased in response to 10 and 20 μM SB203580. (B) PKAα was highly expressed during the cyprid and juvenile stages.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4593178&req=5

f6: Characterization of PKAα expression.(A) Western blot showing that the level of PKAα decreased in response to 10 and 20 μM SB203580. (B) PKAα was highly expressed during the cyprid and juvenile stages.
Mentions: Western blot analysis confirmed that the inhibition of p38 MAPK using SB203580 decreased the level of PKAα (Fig. 6A). Among the developmental stages, PKAα was highly expressed in the cyprid and juvenile stages (Fig. 6B).

Bottom Line: The results showed that the expression levels of 80 proteins were significantly modified (p < 0.05).These differentially expressed proteins were assigned to 15 functional groups according to the KOG database and 9 pathways were significantly enriched.Overall, p38 MAPK might regulate multiple functions in A. amphitrite cyprids, including the energy supply, metamorphosis, neural system and cement glands.

View Article: PubMed Central - PubMed

Affiliation: Environmental Science Programs and Division of Life Science, School of Science, the Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, P. R. China.

ABSTRACT
The MKK3-p38 MAPK pathway has been reported to mediate larval settlement in Amphibalanus (=Balanus) amphitrite. To clarify the underlying molecular mechanism, we applied label-free proteomics to analyze changes in the proteome of cyprids treated with a p38 MAPK inhibitor. The results showed that the expression levels of 80 proteins were significantly modified (p < 0.05). These differentially expressed proteins were assigned to 15 functional groups according to the KOG database and 9 pathways were significantly enriched. Further analysis revealed that p38 MAPK might regulate the energy supply and metamorphosis. Two potential regulatory proteins, CUB-serine protease and PKAα, were both down-regulated in expression. CUB-serine protease localized to postaxial seta 2 and 3, as well as the 4 subterminal sensilla in the antennule. Importantly, it was co-localized with the neuron transmitter serotonin in the sections, suggesting that the CUB-serine protease was present in the neural system. PKAα was highly expressed during the cyprid and juvenile stages, and it was co-localized with phospho-p38 MAPK (pp38 MAPK) to the cement gland, suggesting that PKAα might have some functions in cement glands. Overall, p38 MAPK might regulate multiple functions in A. amphitrite cyprids, including the energy supply, metamorphosis, neural system and cement glands.

No MeSH data available.