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The role of putrescine in the regulation of proteins and fatty acids of thylakoid membranes under salt stress.

Shu S, Yuan Y, Chen J, Sun J, Zhang W, Tang Y, Zhong M, Guo S - Sci Rep (2015)

Bottom Line: Polyamines can alleviate the inhibitory effects of salinity on plant growth by regulating photosynthetic efficiency.Put altered unsaturated fatty acid content, thereby alleviating the disintegration of thylakoid grana lamellae and reducing the number of plastoglobuli in thylakoid membranes.Our results suggest that Put regulates protein expression at transcriptional and translational levels by increasing endogenous polyamines levels in thylakoid membranes, which may stabilise photosynthetic apparatus under salt stress.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.

ABSTRACT
Polyamines can alleviate the inhibitory effects of salinity on plant growth by regulating photosynthetic efficiency. However, little information is available to explain the specific mechanisms underlying the contribution of polyamines to salt tolerance of the photosynthetic apparatus. Here, we investigated the role of putrescine (Put) on the photosynthetic apparatus of cucumber seedlings under salt stress. We found that NaCl stress resulted in severe ion toxicity and oxidative stress in cucumber chloroplasts. In addition, salinity caused a significant increase in the saturated fatty acid contents of thylakoid membranes. Put altered unsaturated fatty acid content, thereby alleviating the disintegration of thylakoid grana lamellae and reducing the number of plastoglobuli in thylakoid membranes. BN-PAGE revealed Put up-regulated the expression of ATP synthase, CP47, D1, Qb, and psbA proteins and down-regulated CP24, D2, and LHCII type III in NaCl-stressed thylakoid membranes. qRT-PCR analysis of gene expression was used to compare transcript and protein accumulation among 10 candidate proteins. For five of these proteins, induced transcript accumulation was consistent with the pattern of induced protein accumulation. Our results suggest that Put regulates protein expression at transcriptional and translational levels by increasing endogenous polyamines levels in thylakoid membranes, which may stabilise photosynthetic apparatus under salt stress.

No MeSH data available.


Related in: MedlinePlus

Effects of exogenous putrescine (Put) on Na+(a) and Cl−(b) concentrations in the chloroplasts of cucumber plants grown in nutrient solutions with or without NaCl for 7 days. Each histogram represents a mean ± SE of three independent experiments. Different letters indicate significant differences between treatments (P < 0.05). The data were taken on the third leaves (numbered basipetally) after the final salt treatment (75 mM NaCl). Cont, 0 mM NaCl + 0 mM Put; Put, 0 mM NaCl + 8 mM Put; NaCl, 75 mM NaCl + 0 mM Put; NaCl+Put, 75 mM NaCl + 8 mM Put.
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f1: Effects of exogenous putrescine (Put) on Na+(a) and Cl−(b) concentrations in the chloroplasts of cucumber plants grown in nutrient solutions with or without NaCl for 7 days. Each histogram represents a mean ± SE of three independent experiments. Different letters indicate significant differences between treatments (P < 0.05). The data were taken on the third leaves (numbered basipetally) after the final salt treatment (75 mM NaCl). Cont, 0 mM NaCl + 0 mM Put; Put, 0 mM NaCl + 8 mM Put; NaCl, 75 mM NaCl + 0 mM Put; NaCl+Put, 75 mM NaCl + 8 mM Put.

Mentions: Na+ and Cl− concentrations in cucumber chloroplasts were measured on day 7 after the final salt treatment (75 mM NaCl) with or without Put. We found that there were significant increases in the contents of Na+ (Fig. 1a) and Cl− (Fig. 1b) of the NaCl-stressed chloroplasts compared with the controls (1386.7% and 166.2% respectively). However, application of 8 mM Put dramatically decreased the levels of accumulated Na+ and Cl− in the salt-stressed chloroplasts. No significant differences were observed in the Na+ and Cl− concentrations of the chloroplasts between the control and the control plus Put treatment.


The role of putrescine in the regulation of proteins and fatty acids of thylakoid membranes under salt stress.

Shu S, Yuan Y, Chen J, Sun J, Zhang W, Tang Y, Zhong M, Guo S - Sci Rep (2015)

Effects of exogenous putrescine (Put) on Na+(a) and Cl−(b) concentrations in the chloroplasts of cucumber plants grown in nutrient solutions with or without NaCl for 7 days. Each histogram represents a mean ± SE of three independent experiments. Different letters indicate significant differences between treatments (P < 0.05). The data were taken on the third leaves (numbered basipetally) after the final salt treatment (75 mM NaCl). Cont, 0 mM NaCl + 0 mM Put; Put, 0 mM NaCl + 8 mM Put; NaCl, 75 mM NaCl + 0 mM Put; NaCl+Put, 75 mM NaCl + 8 mM Put.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4593046&req=5

f1: Effects of exogenous putrescine (Put) on Na+(a) and Cl−(b) concentrations in the chloroplasts of cucumber plants grown in nutrient solutions with or without NaCl for 7 days. Each histogram represents a mean ± SE of three independent experiments. Different letters indicate significant differences between treatments (P < 0.05). The data were taken on the third leaves (numbered basipetally) after the final salt treatment (75 mM NaCl). Cont, 0 mM NaCl + 0 mM Put; Put, 0 mM NaCl + 8 mM Put; NaCl, 75 mM NaCl + 0 mM Put; NaCl+Put, 75 mM NaCl + 8 mM Put.
Mentions: Na+ and Cl− concentrations in cucumber chloroplasts were measured on day 7 after the final salt treatment (75 mM NaCl) with or without Put. We found that there were significant increases in the contents of Na+ (Fig. 1a) and Cl− (Fig. 1b) of the NaCl-stressed chloroplasts compared with the controls (1386.7% and 166.2% respectively). However, application of 8 mM Put dramatically decreased the levels of accumulated Na+ and Cl− in the salt-stressed chloroplasts. No significant differences were observed in the Na+ and Cl− concentrations of the chloroplasts between the control and the control plus Put treatment.

Bottom Line: Polyamines can alleviate the inhibitory effects of salinity on plant growth by regulating photosynthetic efficiency.Put altered unsaturated fatty acid content, thereby alleviating the disintegration of thylakoid grana lamellae and reducing the number of plastoglobuli in thylakoid membranes.Our results suggest that Put regulates protein expression at transcriptional and translational levels by increasing endogenous polyamines levels in thylakoid membranes, which may stabilise photosynthetic apparatus under salt stress.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.

ABSTRACT
Polyamines can alleviate the inhibitory effects of salinity on plant growth by regulating photosynthetic efficiency. However, little information is available to explain the specific mechanisms underlying the contribution of polyamines to salt tolerance of the photosynthetic apparatus. Here, we investigated the role of putrescine (Put) on the photosynthetic apparatus of cucumber seedlings under salt stress. We found that NaCl stress resulted in severe ion toxicity and oxidative stress in cucumber chloroplasts. In addition, salinity caused a significant increase in the saturated fatty acid contents of thylakoid membranes. Put altered unsaturated fatty acid content, thereby alleviating the disintegration of thylakoid grana lamellae and reducing the number of plastoglobuli in thylakoid membranes. BN-PAGE revealed Put up-regulated the expression of ATP synthase, CP47, D1, Qb, and psbA proteins and down-regulated CP24, D2, and LHCII type III in NaCl-stressed thylakoid membranes. qRT-PCR analysis of gene expression was used to compare transcript and protein accumulation among 10 candidate proteins. For five of these proteins, induced transcript accumulation was consistent with the pattern of induced protein accumulation. Our results suggest that Put regulates protein expression at transcriptional and translational levels by increasing endogenous polyamines levels in thylakoid membranes, which may stabilise photosynthetic apparatus under salt stress.

No MeSH data available.


Related in: MedlinePlus