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Efficient production of lumichrome by Microbacterium sp. strain TPU 3598.

Yamamoto K, Asano Y - Appl. Environ. Microbiol. (2015)

Bottom Line: In the resting cell method, 20 g of cells (wet weight) in 100 ml of potassium phosphate buffer, pH 7.0, produced 2.4 g of lumichrome from 3.8 g of riboflavin (98% yield).Since the lumichrome production by these methods was carried out in suspension, the resulting lumichrome was easily purified from the cultivation medium or reaction mixture by centrifugation and crystallization.Thus, the biochemical methods we describe here are a significant improvement in terms of simplicity and yield over the existing chemical, photolytic, and other biochemical methods of lumichrome production.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Kurokawa, Imizu, Toyama, Japan; Asano Active Enzyme Molecule Project, ERATO, JST, Kurokawa, Imizu, Toyama, Japan.

No MeSH data available.


Related in: MedlinePlus

Effect of pH on lumichrome production. (A) Cultivation method. The isolated strain was cultivated at 30°C for 10 h in 10 ml of nutrient medium containing 1.0 μmol riboflavin. (B) Resting cell method. Lumichrome production was carried out with 200 mg of cells at 30°C for 8 h in 10 ml of buffer containing 50 μmol riboflavin. ●, acetate buffer; ◆, KPB; ▲, Tris-HCl buffer; ■, Gly-NaOH buffer. Error bars indicate the standard deviations from three replicates.
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Figure 4: Effect of pH on lumichrome production. (A) Cultivation method. The isolated strain was cultivated at 30°C for 10 h in 10 ml of nutrient medium containing 1.0 μmol riboflavin. (B) Resting cell method. Lumichrome production was carried out with 200 mg of cells at 30°C for 8 h in 10 ml of buffer containing 50 μmol riboflavin. ●, acetate buffer; ◆, KPB; ▲, Tris-HCl buffer; ■, Gly-NaOH buffer. Error bars indicate the standard deviations from three replicates.

Mentions: The optimal pH of the cultivation method was determined by testing a pH range of 5.0 to 9.0 as described in Materials and Methods. Cell growth and lumichrome production at pH 5.0 were much lower than those at the other pH values (0.4% yield). The strain grew well in the pH range from 6.0 to 9.0, and levels of lumichrome production between pH 7.0 and 9.0 were similar to each other, while the production level at pH 6.0 was lower than that at pH 7.0 to 9.0 (Fig. 4A). Therefore, we selected pH 7.0 as the optimal pH for the production of lumichrome by the cultivation method.


Efficient production of lumichrome by Microbacterium sp. strain TPU 3598.

Yamamoto K, Asano Y - Appl. Environ. Microbiol. (2015)

Effect of pH on lumichrome production. (A) Cultivation method. The isolated strain was cultivated at 30°C for 10 h in 10 ml of nutrient medium containing 1.0 μmol riboflavin. (B) Resting cell method. Lumichrome production was carried out with 200 mg of cells at 30°C for 8 h in 10 ml of buffer containing 50 μmol riboflavin. ●, acetate buffer; ◆, KPB; ▲, Tris-HCl buffer; ■, Gly-NaOH buffer. Error bars indicate the standard deviations from three replicates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592859&req=5

Figure 4: Effect of pH on lumichrome production. (A) Cultivation method. The isolated strain was cultivated at 30°C for 10 h in 10 ml of nutrient medium containing 1.0 μmol riboflavin. (B) Resting cell method. Lumichrome production was carried out with 200 mg of cells at 30°C for 8 h in 10 ml of buffer containing 50 μmol riboflavin. ●, acetate buffer; ◆, KPB; ▲, Tris-HCl buffer; ■, Gly-NaOH buffer. Error bars indicate the standard deviations from three replicates.
Mentions: The optimal pH of the cultivation method was determined by testing a pH range of 5.0 to 9.0 as described in Materials and Methods. Cell growth and lumichrome production at pH 5.0 were much lower than those at the other pH values (0.4% yield). The strain grew well in the pH range from 6.0 to 9.0, and levels of lumichrome production between pH 7.0 and 9.0 were similar to each other, while the production level at pH 6.0 was lower than that at pH 7.0 to 9.0 (Fig. 4A). Therefore, we selected pH 7.0 as the optimal pH for the production of lumichrome by the cultivation method.

Bottom Line: In the resting cell method, 20 g of cells (wet weight) in 100 ml of potassium phosphate buffer, pH 7.0, produced 2.4 g of lumichrome from 3.8 g of riboflavin (98% yield).Since the lumichrome production by these methods was carried out in suspension, the resulting lumichrome was easily purified from the cultivation medium or reaction mixture by centrifugation and crystallization.Thus, the biochemical methods we describe here are a significant improvement in terms of simplicity and yield over the existing chemical, photolytic, and other biochemical methods of lumichrome production.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Kurokawa, Imizu, Toyama, Japan; Asano Active Enzyme Molecule Project, ERATO, JST, Kurokawa, Imizu, Toyama, Japan.

No MeSH data available.


Related in: MedlinePlus