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Effect of Selenium Supplementation on Redox Status of the Aortic Wall in Young Spontaneously Hypertensive Rats.

Ruseva B, Atanasova M, Tsvetkova R, Betova T, Mollova M, Alexandrova M, Laleva P, Dimitrova A - Oxid Med Cell Longev (2015)

Bottom Line: Se supplementation significantly increased GPx-1 activity of whole blood and in the aortas of WKY and SHR.Decreased lipid peroxidation level, eNOS-3 expression in the aortic wall, and serum level of anti-AGEs abs were found in SHR HSe compared with SHR NSe.In conclusion, Se supplementation improved the redox status of the aortic wall in young SHR.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria.

ABSTRACT
Selenium (Se) is an exogenous antioxidant that performs its function via the expression of selenoproteins. The aim of this study was to explore the effect of varying Se intake on the redox status of the aortic wall in young spontaneously hypertensive rats (SHR). Sixteen male Wistar Kyoto (WKY) rats and nineteen male SHR, 16-week-old, were tested after being given diets with different Se content for eight weeks. They were divided into 4 groups: control groups of WKY NSe and SHR NSe on an adequate Se diet and groups of WKY HSe and SHR HSe that received Se supplementation. The Se nutritional status was assessed by measuring whole blood glutathione peroxidase-1 (GPx-1) activity. Serum concentration of lipid hydroperoxides and serum level of antibodies against advanced glycation end products (anti-AGEs abs) were determined. Expression of GPx-1 and endothelial nitric oxide synthase (eNOS) were examined in aortic wall. Se supplementation significantly increased GPx-1 activity of whole blood and in the aortas of WKY and SHR. Decreased lipid peroxidation level, eNOS-3 expression in the aortic wall, and serum level of anti-AGEs abs were found in SHR HSe compared with SHR NSe. In conclusion, Se supplementation improved the redox status of the aortic wall in young SHR.

No MeSH data available.


A representative photomicrograph of aortic wall of WKY on high selenium content diet (thin arrow: endothelium; thick arrow: media). (a) Histology, intact endothelium and normal thickness of aortic wall (magnification ×400). (b) Expression of GPx-1, immunofluorescent lighting with high intensity (magnification ×400).
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fig5: A representative photomicrograph of aortic wall of WKY on high selenium content diet (thin arrow: endothelium; thick arrow: media). (a) Histology, intact endothelium and normal thickness of aortic wall (magnification ×400). (b) Expression of GPx-1, immunofluorescent lighting with high intensity (magnification ×400).

Mentions: The degree of determined GPx-1 expression in the aortic wall depended proportionally on the selenium content of the applied diets (Figures 4–7).


Effect of Selenium Supplementation on Redox Status of the Aortic Wall in Young Spontaneously Hypertensive Rats.

Ruseva B, Atanasova M, Tsvetkova R, Betova T, Mollova M, Alexandrova M, Laleva P, Dimitrova A - Oxid Med Cell Longev (2015)

A representative photomicrograph of aortic wall of WKY on high selenium content diet (thin arrow: endothelium; thick arrow: media). (a) Histology, intact endothelium and normal thickness of aortic wall (magnification ×400). (b) Expression of GPx-1, immunofluorescent lighting with high intensity (magnification ×400).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4592724&req=5

fig5: A representative photomicrograph of aortic wall of WKY on high selenium content diet (thin arrow: endothelium; thick arrow: media). (a) Histology, intact endothelium and normal thickness of aortic wall (magnification ×400). (b) Expression of GPx-1, immunofluorescent lighting with high intensity (magnification ×400).
Mentions: The degree of determined GPx-1 expression in the aortic wall depended proportionally on the selenium content of the applied diets (Figures 4–7).

Bottom Line: Se supplementation significantly increased GPx-1 activity of whole blood and in the aortas of WKY and SHR.Decreased lipid peroxidation level, eNOS-3 expression in the aortic wall, and serum level of anti-AGEs abs were found in SHR HSe compared with SHR NSe.In conclusion, Se supplementation improved the redox status of the aortic wall in young SHR.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria.

ABSTRACT
Selenium (Se) is an exogenous antioxidant that performs its function via the expression of selenoproteins. The aim of this study was to explore the effect of varying Se intake on the redox status of the aortic wall in young spontaneously hypertensive rats (SHR). Sixteen male Wistar Kyoto (WKY) rats and nineteen male SHR, 16-week-old, were tested after being given diets with different Se content for eight weeks. They were divided into 4 groups: control groups of WKY NSe and SHR NSe on an adequate Se diet and groups of WKY HSe and SHR HSe that received Se supplementation. The Se nutritional status was assessed by measuring whole blood glutathione peroxidase-1 (GPx-1) activity. Serum concentration of lipid hydroperoxides and serum level of antibodies against advanced glycation end products (anti-AGEs abs) were determined. Expression of GPx-1 and endothelial nitric oxide synthase (eNOS) were examined in aortic wall. Se supplementation significantly increased GPx-1 activity of whole blood and in the aortas of WKY and SHR. Decreased lipid peroxidation level, eNOS-3 expression in the aortic wall, and serum level of anti-AGEs abs were found in SHR HSe compared with SHR NSe. In conclusion, Se supplementation improved the redox status of the aortic wall in young SHR.

No MeSH data available.