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Cellular Expression of Cyclooxygenase, Aromatase, Adipokines, Inflammation and Cell Proliferation Markers in Breast Cancer Specimen.

Basu S, Combe K, Kwiatkowski F, Caldefie-Chézet F, Penault-Llorca F, Bignon YJ, Vasson MP - PLoS ONE (2015)

Bottom Line: AdipoR1, adiponectin, Ob-R, leptin, COX-1, COX-2, aromatase, PGF2α, F2-isoprostanes and α-SMA were localised on higher levels in the breast tissues adjacent to the tumor compared to tumor specimens when considering either score or staining area whereas COX-2 and AdipoR2 were found to be higher considering staining intensity and Ki67 on score level in the tumor tissue.There was no significant difference observed on β-catenin either on score nor on staining area and intensity between tissues adjacent to the tumor and tumor tissues.A positive correlation was found between COX-1 and COX-2 in the tumor tissues.

View Article: PubMed Central - PubMed

Affiliation: Clermont Université, Université d'Auvergne, UMR 1019, Unité de Nutrition Humaine, CRNH-Auvergne, BP 10448, F-63000, Clermont-Ferrand, France; Oxidative Stress and Inflammation, Department of Public Health and Caring Sciences, Faculty of Medicine, Uppsala University, Uppsala, Sweden.

ABSTRACT
Current evidences suggest that expression of Ki67, cyclooxygenase (COX), aromatase, adipokines, prostaglandins, free radicals, β-catenin and α-SMA might be involved in breast cancer pathogenesis. The main objective of this study was to compare expression/localization of these potential compounds in breast cancer tissues with tissues collected adjacent to the tumor using immunohistochemistry and correlated with clinical pathology. The breast cancer specimens were collected from 30 women aged between 49 and 89 years who underwent breast surgery following cancer diagnosis. Expression levels of molecules by different stainings were graded as a score on a scale based upon staining intensity and proportion of positive cells/area or individually. AdipoR1, adiponectin, Ob-R, leptin, COX-1, COX-2, aromatase, PGF2α, F2-isoprostanes and α-SMA were localised on higher levels in the breast tissues adjacent to the tumor compared to tumor specimens when considering either score or staining area whereas COX-2 and AdipoR2 were found to be higher considering staining intensity and Ki67 on score level in the tumor tissue. There was no significant difference observed on β-catenin either on score nor on staining area and intensity between tissues adjacent to the tumor and tumor tissues. A positive correlation was found between COX-1 and COX-2 in the tumor tissues. In conclusion, these suggest that Ki67, COXs, aromatase, prostaglandin, free radicals, adipokines, β-catenin and α-SMA are involved in breast cancer. These further focus the need of examination of tissues adjacent to tumor, tumor itself and compare them with normal or benign breast tissues for a better understanding of breast cancer pathology and future evaluation of therapeutic benefit.

No MeSH data available.


Related in: MedlinePlus

F2-isoprostane and PGF2α immunostaning in adjacent breast tissue to tumor or breast tumor tissue.Adjacent breast tissue to tumor or breast tumor tissue labeled by indirect immunofluorescence for A/ F2-isoprostane and B/ PGF2α (green) with DAPI as nuclear counterstain (blue).
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pone.0138443.g005: F2-isoprostane and PGF2α immunostaning in adjacent breast tissue to tumor or breast tumor tissue.Adjacent breast tissue to tumor or breast tumor tissue labeled by indirect immunofluorescence for A/ F2-isoprostane and B/ PGF2α (green) with DAPI as nuclear counterstain (blue).

Mentions: COX-1 values were lower in the tumor tissues than tissues adjacent to the tumor (p = 0.0015) on the percent of stained area. There was less COX-2 in the tumor than in the tissues adjacent to the tumor (with a large SEM) (p = 0.0096) considering the percent area stained as seen for the score above. However when considering the intensity, COX-2 values are significantly lower (p = 0.009) for tissues adjacent to the tumor compared to tumor tissues. F2-isoprostanes and PGF2α metabolite levels were higher both on the percent of area stained (F2-isoprostanes, p = 0.0048; PGF2α, p = 0.012) and intensity levels in the tissues adjacent to the tumor than tumor tissues (F2-isoprostanes, p = 0.025; PGF2α, p = 0.037). Fig 5 shows adjacent breast tissue to tumor (left panel) and breast tumor tissues (right panel) labeled by indirect immunofluorescence for A) F2-isoprostanes and B) PGF2α with DAPI as nuclear counterstain.


Cellular Expression of Cyclooxygenase, Aromatase, Adipokines, Inflammation and Cell Proliferation Markers in Breast Cancer Specimen.

Basu S, Combe K, Kwiatkowski F, Caldefie-Chézet F, Penault-Llorca F, Bignon YJ, Vasson MP - PLoS ONE (2015)

F2-isoprostane and PGF2α immunostaning in adjacent breast tissue to tumor or breast tumor tissue.Adjacent breast tissue to tumor or breast tumor tissue labeled by indirect immunofluorescence for A/ F2-isoprostane and B/ PGF2α (green) with DAPI as nuclear counterstain (blue).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592217&req=5

pone.0138443.g005: F2-isoprostane and PGF2α immunostaning in adjacent breast tissue to tumor or breast tumor tissue.Adjacent breast tissue to tumor or breast tumor tissue labeled by indirect immunofluorescence for A/ F2-isoprostane and B/ PGF2α (green) with DAPI as nuclear counterstain (blue).
Mentions: COX-1 values were lower in the tumor tissues than tissues adjacent to the tumor (p = 0.0015) on the percent of stained area. There was less COX-2 in the tumor than in the tissues adjacent to the tumor (with a large SEM) (p = 0.0096) considering the percent area stained as seen for the score above. However when considering the intensity, COX-2 values are significantly lower (p = 0.009) for tissues adjacent to the tumor compared to tumor tissues. F2-isoprostanes and PGF2α metabolite levels were higher both on the percent of area stained (F2-isoprostanes, p = 0.0048; PGF2α, p = 0.012) and intensity levels in the tissues adjacent to the tumor than tumor tissues (F2-isoprostanes, p = 0.025; PGF2α, p = 0.037). Fig 5 shows adjacent breast tissue to tumor (left panel) and breast tumor tissues (right panel) labeled by indirect immunofluorescence for A) F2-isoprostanes and B) PGF2α with DAPI as nuclear counterstain.

Bottom Line: AdipoR1, adiponectin, Ob-R, leptin, COX-1, COX-2, aromatase, PGF2α, F2-isoprostanes and α-SMA were localised on higher levels in the breast tissues adjacent to the tumor compared to tumor specimens when considering either score or staining area whereas COX-2 and AdipoR2 were found to be higher considering staining intensity and Ki67 on score level in the tumor tissue.There was no significant difference observed on β-catenin either on score nor on staining area and intensity between tissues adjacent to the tumor and tumor tissues.A positive correlation was found between COX-1 and COX-2 in the tumor tissues.

View Article: PubMed Central - PubMed

Affiliation: Clermont Université, Université d'Auvergne, UMR 1019, Unité de Nutrition Humaine, CRNH-Auvergne, BP 10448, F-63000, Clermont-Ferrand, France; Oxidative Stress and Inflammation, Department of Public Health and Caring Sciences, Faculty of Medicine, Uppsala University, Uppsala, Sweden.

ABSTRACT
Current evidences suggest that expression of Ki67, cyclooxygenase (COX), aromatase, adipokines, prostaglandins, free radicals, β-catenin and α-SMA might be involved in breast cancer pathogenesis. The main objective of this study was to compare expression/localization of these potential compounds in breast cancer tissues with tissues collected adjacent to the tumor using immunohistochemistry and correlated with clinical pathology. The breast cancer specimens were collected from 30 women aged between 49 and 89 years who underwent breast surgery following cancer diagnosis. Expression levels of molecules by different stainings were graded as a score on a scale based upon staining intensity and proportion of positive cells/area or individually. AdipoR1, adiponectin, Ob-R, leptin, COX-1, COX-2, aromatase, PGF2α, F2-isoprostanes and α-SMA were localised on higher levels in the breast tissues adjacent to the tumor compared to tumor specimens when considering either score or staining area whereas COX-2 and AdipoR2 were found to be higher considering staining intensity and Ki67 on score level in the tumor tissue. There was no significant difference observed on β-catenin either on score nor on staining area and intensity between tissues adjacent to the tumor and tumor tissues. A positive correlation was found between COX-1 and COX-2 in the tumor tissues. In conclusion, these suggest that Ki67, COXs, aromatase, prostaglandin, free radicals, adipokines, β-catenin and α-SMA are involved in breast cancer. These further focus the need of examination of tissues adjacent to tumor, tumor itself and compare them with normal or benign breast tissues for a better understanding of breast cancer pathology and future evaluation of therapeutic benefit.

No MeSH data available.


Related in: MedlinePlus