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Antimicrobial Resistance and Molecular Investigation of H2S-Negative Salmonella enterica subsp. enterica serovar Choleraesuis Isolates in China.

Xie J, Yi S, Zhu J, Li P, Liang B, Li H, Yang X, Wang L, Hao R, Jia L, Wu Z, Qiu S, Song H - PLoS ONE (2015)

Bottom Line: Fifteen isolates were found to be multidrug resistant.By MLST analysis, the H2S-negative isolates were all found to belong to ST68 and H2S-positive isolates belong to ST145.By CRISPR analysis, no significant differences in CRISPR 1 were detected; however, one H2S-negative isolate was found to contain three new spacers in CRISPR 2.

View Article: PubMed Central - PubMed

Affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.

ABSTRACT
Salmonella enterica subsp. enterica serovar Choleraesuis is a highly invasive pathogen of swine that frequently causes serious outbreaks, in particular in Asia, and can also cause severe invasive disease in humans. In this study, 21 S. Choleraesuis isolates, detected from 21 patients with diarrhea in China between 2010 and 2011, were found to include 19 H2S-negative S. Choleraesuis isolates and two H2S-positive isolates. This is the first report of H2S-negative S. Choleraesuis isolated from humans. The majority of H2S-negative isolates exhibited high resistance to ampicillin, chloramphenicol, gentamicin, tetracycline, ticarcillin, and trimethoprim-sulfamethoxazole, but only six isolates were resistant to norfloxacin. In contrast, all of the isolates were sensitive to cephalosporins. Fifteen isolates were found to be multidrug resistant. In norfloxacin-resistant isolates, we detected mutations in the gyrA and parC genes and identified two new mutations in the parC gene. Pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and clustered regularly interspaced short palindromic repeat (CRISPR) analysis were employed to investigate the genetic relatedness of H2S-negative and H2S-positive S. Choleraesuis isolates. PFGE revealed two groups, with all 19 H2S-negative S. Choleraesuis isolates belonging to Group I and H2S-positive isolates belonging to Group II. By MLST analysis, the H2S-negative isolates were all found to belong to ST68 and H2S-positive isolates belong to ST145. By CRISPR analysis, no significant differences in CRISPR 1 were detected; however, one H2S-negative isolate was found to contain three new spacers in CRISPR 2. All 19 H2S-negative isolates also possessed a frame-shift mutation at position 760 of phsA gene compared with H2S-positive isolates, which may be responsible for the H2S-negative phenotype. Moreover, the 19 H2S-negative isolates have similar PFGE patterns and same mutation site in the phsA gene, these results indicated that these H2S-negative isolates may have been prevalent in China. These findings suggested that surveillance should be increased of H2S-negative S. Choleraesuis in China.

No MeSH data available.


Related in: MedlinePlus

Alignment of phsA sequences in 21 S. Choleraesuis.The deletion of G at position 760 resulted in a frame-shift mutation. The first sequence is H2S-positive S. Choleraesuis strain SC-B67 (NC_006905.1).
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pone.0139115.g003: Alignment of phsA sequences in 21 S. Choleraesuis.The deletion of G at position 760 resulted in a frame-shift mutation. The first sequence is H2S-positive S. Choleraesuis strain SC-B67 (NC_006905.1).

Mentions: We analyzed the phs operon of all 21 S. Choleraesuis isolates and compared it with that of S. Choleraesuis strain SC-B67. In all 19 H2S-negative isolates, a single base deletion was detected at position 760 in the phsA gene (Fig 3). This deletion led to a frame-shift mutation, resulting in a nonfunctional protein that may be unable to generate H2S. No mutations in the phsB and phsC genes were detected (data not shown). These results suggested that the H2S-negative phenotype may result of the frame-shift mutation at position 760 in the phsA gene (GenBank accession numbers: KP184398–184416, 18419–18420).


Antimicrobial Resistance and Molecular Investigation of H2S-Negative Salmonella enterica subsp. enterica serovar Choleraesuis Isolates in China.

Xie J, Yi S, Zhu J, Li P, Liang B, Li H, Yang X, Wang L, Hao R, Jia L, Wu Z, Qiu S, Song H - PLoS ONE (2015)

Alignment of phsA sequences in 21 S. Choleraesuis.The deletion of G at position 760 resulted in a frame-shift mutation. The first sequence is H2S-positive S. Choleraesuis strain SC-B67 (NC_006905.1).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592067&req=5

pone.0139115.g003: Alignment of phsA sequences in 21 S. Choleraesuis.The deletion of G at position 760 resulted in a frame-shift mutation. The first sequence is H2S-positive S. Choleraesuis strain SC-B67 (NC_006905.1).
Mentions: We analyzed the phs operon of all 21 S. Choleraesuis isolates and compared it with that of S. Choleraesuis strain SC-B67. In all 19 H2S-negative isolates, a single base deletion was detected at position 760 in the phsA gene (Fig 3). This deletion led to a frame-shift mutation, resulting in a nonfunctional protein that may be unable to generate H2S. No mutations in the phsB and phsC genes were detected (data not shown). These results suggested that the H2S-negative phenotype may result of the frame-shift mutation at position 760 in the phsA gene (GenBank accession numbers: KP184398–184416, 18419–18420).

Bottom Line: Fifteen isolates were found to be multidrug resistant.By MLST analysis, the H2S-negative isolates were all found to belong to ST68 and H2S-positive isolates belong to ST145.By CRISPR analysis, no significant differences in CRISPR 1 were detected; however, one H2S-negative isolate was found to contain three new spacers in CRISPR 2.

View Article: PubMed Central - PubMed

Affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.

ABSTRACT
Salmonella enterica subsp. enterica serovar Choleraesuis is a highly invasive pathogen of swine that frequently causes serious outbreaks, in particular in Asia, and can also cause severe invasive disease in humans. In this study, 21 S. Choleraesuis isolates, detected from 21 patients with diarrhea in China between 2010 and 2011, were found to include 19 H2S-negative S. Choleraesuis isolates and two H2S-positive isolates. This is the first report of H2S-negative S. Choleraesuis isolated from humans. The majority of H2S-negative isolates exhibited high resistance to ampicillin, chloramphenicol, gentamicin, tetracycline, ticarcillin, and trimethoprim-sulfamethoxazole, but only six isolates were resistant to norfloxacin. In contrast, all of the isolates were sensitive to cephalosporins. Fifteen isolates were found to be multidrug resistant. In norfloxacin-resistant isolates, we detected mutations in the gyrA and parC genes and identified two new mutations in the parC gene. Pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and clustered regularly interspaced short palindromic repeat (CRISPR) analysis were employed to investigate the genetic relatedness of H2S-negative and H2S-positive S. Choleraesuis isolates. PFGE revealed two groups, with all 19 H2S-negative S. Choleraesuis isolates belonging to Group I and H2S-positive isolates belonging to Group II. By MLST analysis, the H2S-negative isolates were all found to belong to ST68 and H2S-positive isolates belong to ST145. By CRISPR analysis, no significant differences in CRISPR 1 were detected; however, one H2S-negative isolate was found to contain three new spacers in CRISPR 2. All 19 H2S-negative isolates also possessed a frame-shift mutation at position 760 of phsA gene compared with H2S-positive isolates, which may be responsible for the H2S-negative phenotype. Moreover, the 19 H2S-negative isolates have similar PFGE patterns and same mutation site in the phsA gene, these results indicated that these H2S-negative isolates may have been prevalent in China. These findings suggested that surveillance should be increased of H2S-negative S. Choleraesuis in China.

No MeSH data available.


Related in: MedlinePlus