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Antimicrobial Resistance and Molecular Investigation of H2S-Negative Salmonella enterica subsp. enterica serovar Choleraesuis Isolates in China.

Xie J, Yi S, Zhu J, Li P, Liang B, Li H, Yang X, Wang L, Hao R, Jia L, Wu Z, Qiu S, Song H - PLoS ONE (2015)

Bottom Line: Fifteen isolates were found to be multidrug resistant.By MLST analysis, the H2S-negative isolates were all found to belong to ST68 and H2S-positive isolates belong to ST145.By CRISPR analysis, no significant differences in CRISPR 1 were detected; however, one H2S-negative isolate was found to contain three new spacers in CRISPR 2.

View Article: PubMed Central - PubMed

Affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.

ABSTRACT
Salmonella enterica subsp. enterica serovar Choleraesuis is a highly invasive pathogen of swine that frequently causes serious outbreaks, in particular in Asia, and can also cause severe invasive disease in humans. In this study, 21 S. Choleraesuis isolates, detected from 21 patients with diarrhea in China between 2010 and 2011, were found to include 19 H2S-negative S. Choleraesuis isolates and two H2S-positive isolates. This is the first report of H2S-negative S. Choleraesuis isolated from humans. The majority of H2S-negative isolates exhibited high resistance to ampicillin, chloramphenicol, gentamicin, tetracycline, ticarcillin, and trimethoprim-sulfamethoxazole, but only six isolates were resistant to norfloxacin. In contrast, all of the isolates were sensitive to cephalosporins. Fifteen isolates were found to be multidrug resistant. In norfloxacin-resistant isolates, we detected mutations in the gyrA and parC genes and identified two new mutations in the parC gene. Pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and clustered regularly interspaced short palindromic repeat (CRISPR) analysis were employed to investigate the genetic relatedness of H2S-negative and H2S-positive S. Choleraesuis isolates. PFGE revealed two groups, with all 19 H2S-negative S. Choleraesuis isolates belonging to Group I and H2S-positive isolates belonging to Group II. By MLST analysis, the H2S-negative isolates were all found to belong to ST68 and H2S-positive isolates belong to ST145. By CRISPR analysis, no significant differences in CRISPR 1 were detected; however, one H2S-negative isolate was found to contain three new spacers in CRISPR 2. All 19 H2S-negative isolates also possessed a frame-shift mutation at position 760 of phsA gene compared with H2S-positive isolates, which may be responsible for the H2S-negative phenotype. Moreover, the 19 H2S-negative isolates have similar PFGE patterns and same mutation site in the phsA gene, these results indicated that these H2S-negative isolates may have been prevalent in China. These findings suggested that surveillance should be increased of H2S-negative S. Choleraesuis in China.

No MeSH data available.


Related in: MedlinePlus

Dendrogram analysis of PFGE for the 21 S. Choleraesuis isolates by XbaI-digestion.The strain number, species, origin and ST are shown for each isolate.
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pone.0139115.g002: Dendrogram analysis of PFGE for the 21 S. Choleraesuis isolates by XbaI-digestion.The strain number, species, origin and ST are shown for each isolate.

Mentions: The 21 S. Choleraesuis isolates were subtyped into 16 unique XbaI PFGE patterns (Fig 2). The 19 H2S-negative isolates produced 14 different profiles, while the two H2S-positive isolates produced two profiles. Cluster analysis of the PFGE patterns revealed two main distinct groups accounting for approximately 70% pattern similarity. In Group I, all of the 19 isolates, belonging to ST68, were H2S-negative. This group included three isolates from Shanghai (SC1209, SC1214, and SC1220) and four from Nanjing (SC1201–1204) that shared approximately 96% similarity in their PFGE patterns. This finding indicated that H2S-negative S. Choleraesuis isolates belonging to ST68 have the potential for cross-region dissemination. In Group II, there were only two isolates, which belonging to ST145 were identified as H2S-positive, and shared approximately 96% similarity as well.


Antimicrobial Resistance and Molecular Investigation of H2S-Negative Salmonella enterica subsp. enterica serovar Choleraesuis Isolates in China.

Xie J, Yi S, Zhu J, Li P, Liang B, Li H, Yang X, Wang L, Hao R, Jia L, Wu Z, Qiu S, Song H - PLoS ONE (2015)

Dendrogram analysis of PFGE for the 21 S. Choleraesuis isolates by XbaI-digestion.The strain number, species, origin and ST are shown for each isolate.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592067&req=5

pone.0139115.g002: Dendrogram analysis of PFGE for the 21 S. Choleraesuis isolates by XbaI-digestion.The strain number, species, origin and ST are shown for each isolate.
Mentions: The 21 S. Choleraesuis isolates were subtyped into 16 unique XbaI PFGE patterns (Fig 2). The 19 H2S-negative isolates produced 14 different profiles, while the two H2S-positive isolates produced two profiles. Cluster analysis of the PFGE patterns revealed two main distinct groups accounting for approximately 70% pattern similarity. In Group I, all of the 19 isolates, belonging to ST68, were H2S-negative. This group included three isolates from Shanghai (SC1209, SC1214, and SC1220) and four from Nanjing (SC1201–1204) that shared approximately 96% similarity in their PFGE patterns. This finding indicated that H2S-negative S. Choleraesuis isolates belonging to ST68 have the potential for cross-region dissemination. In Group II, there were only two isolates, which belonging to ST145 were identified as H2S-positive, and shared approximately 96% similarity as well.

Bottom Line: Fifteen isolates were found to be multidrug resistant.By MLST analysis, the H2S-negative isolates were all found to belong to ST68 and H2S-positive isolates belong to ST145.By CRISPR analysis, no significant differences in CRISPR 1 were detected; however, one H2S-negative isolate was found to contain three new spacers in CRISPR 2.

View Article: PubMed Central - PubMed

Affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.

ABSTRACT
Salmonella enterica subsp. enterica serovar Choleraesuis is a highly invasive pathogen of swine that frequently causes serious outbreaks, in particular in Asia, and can also cause severe invasive disease in humans. In this study, 21 S. Choleraesuis isolates, detected from 21 patients with diarrhea in China between 2010 and 2011, were found to include 19 H2S-negative S. Choleraesuis isolates and two H2S-positive isolates. This is the first report of H2S-negative S. Choleraesuis isolated from humans. The majority of H2S-negative isolates exhibited high resistance to ampicillin, chloramphenicol, gentamicin, tetracycline, ticarcillin, and trimethoprim-sulfamethoxazole, but only six isolates were resistant to norfloxacin. In contrast, all of the isolates were sensitive to cephalosporins. Fifteen isolates were found to be multidrug resistant. In norfloxacin-resistant isolates, we detected mutations in the gyrA and parC genes and identified two new mutations in the parC gene. Pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and clustered regularly interspaced short palindromic repeat (CRISPR) analysis were employed to investigate the genetic relatedness of H2S-negative and H2S-positive S. Choleraesuis isolates. PFGE revealed two groups, with all 19 H2S-negative S. Choleraesuis isolates belonging to Group I and H2S-positive isolates belonging to Group II. By MLST analysis, the H2S-negative isolates were all found to belong to ST68 and H2S-positive isolates belong to ST145. By CRISPR analysis, no significant differences in CRISPR 1 were detected; however, one H2S-negative isolate was found to contain three new spacers in CRISPR 2. All 19 H2S-negative isolates also possessed a frame-shift mutation at position 760 of phsA gene compared with H2S-positive isolates, which may be responsible for the H2S-negative phenotype. Moreover, the 19 H2S-negative isolates have similar PFGE patterns and same mutation site in the phsA gene, these results indicated that these H2S-negative isolates may have been prevalent in China. These findings suggested that surveillance should be increased of H2S-negative S. Choleraesuis in China.

No MeSH data available.


Related in: MedlinePlus