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Metagenomic Human Repiratory Air in a Hospital Environment.

Lai YY, Li Y, Lang J, Tong X, Zhang L, Fang J, Xing J, Cai M, Xu H, Deng Y, Xiao F, Tian G - PLoS ONE (2015)

Bottom Line: Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies.To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study.It is also potential as a transmissible pathogen real-time modelling system worldwide.

View Article: PubMed Central - PubMed

Affiliation: Genomics and Synthetic Biology Center, Tsinghua University, Beijing, China.

ABSTRACT
Hospital-acquired infection (HAI) or nosocomial infection is an issue that frequent hospital environment. We believe conventional regulated Petri dish method is insufficient to evaluate HAI. To address this problem, metagenomic sequencing was applied to screen airborne microbes in four rooms of Beijing Hospital. With air-in amount of sampler being setup to one person's respiration quantity, metagenomic sequencing identified huge numbers of species in the rooms which had already qualified widely accepted petridish exposing standard, imposing urgency for new technology. Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies. To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study. It is also potential as a transmissible pathogen real-time modelling system worldwide.

No MeSH data available.


Related in: MedlinePlus

PCoA analysis for indoor and outdoor air.Data were normalized between 0 and 1 and compared with PM2.5 and PM10 data set[47]. (red: Indoor results; blue: outdoor results).
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pone.0139044.g004: PCoA analysis for indoor and outdoor air.Data were normalized between 0 and 1 and compared with PM2.5 and PM10 data set[47]. (red: Indoor results; blue: outdoor results).

Mentions: The Principal coordinates analysis (PCoA) (Fig 4) revealed the significant difference of the four rooms we sampled with the outdoor metagenomic sequencing results from other literature [47], indicating that the indoor airborne microorganism pattern is quite different with that of outdoor pattern. Only 36 species cross indoor air and outdoor air as in S3 Table [47] among thousands of species.


Metagenomic Human Repiratory Air in a Hospital Environment.

Lai YY, Li Y, Lang J, Tong X, Zhang L, Fang J, Xing J, Cai M, Xu H, Deng Y, Xiao F, Tian G - PLoS ONE (2015)

PCoA analysis for indoor and outdoor air.Data were normalized between 0 and 1 and compared with PM2.5 and PM10 data set[47]. (red: Indoor results; blue: outdoor results).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592066&req=5

pone.0139044.g004: PCoA analysis for indoor and outdoor air.Data were normalized between 0 and 1 and compared with PM2.5 and PM10 data set[47]. (red: Indoor results; blue: outdoor results).
Mentions: The Principal coordinates analysis (PCoA) (Fig 4) revealed the significant difference of the four rooms we sampled with the outdoor metagenomic sequencing results from other literature [47], indicating that the indoor airborne microorganism pattern is quite different with that of outdoor pattern. Only 36 species cross indoor air and outdoor air as in S3 Table [47] among thousands of species.

Bottom Line: Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies.To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study.It is also potential as a transmissible pathogen real-time modelling system worldwide.

View Article: PubMed Central - PubMed

Affiliation: Genomics and Synthetic Biology Center, Tsinghua University, Beijing, China.

ABSTRACT
Hospital-acquired infection (HAI) or nosocomial infection is an issue that frequent hospital environment. We believe conventional regulated Petri dish method is insufficient to evaluate HAI. To address this problem, metagenomic sequencing was applied to screen airborne microbes in four rooms of Beijing Hospital. With air-in amount of sampler being setup to one person's respiration quantity, metagenomic sequencing identified huge numbers of species in the rooms which had already qualified widely accepted petridish exposing standard, imposing urgency for new technology. Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies. To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study. It is also potential as a transmissible pathogen real-time modelling system worldwide.

No MeSH data available.


Related in: MedlinePlus