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Metagenomic Human Repiratory Air in a Hospital Environment.

Lai YY, Li Y, Lang J, Tong X, Zhang L, Fang J, Xing J, Cai M, Xu H, Deng Y, Xiao F, Tian G - PLoS ONE (2015)

Bottom Line: Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies.To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study.It is also potential as a transmissible pathogen real-time modelling system worldwide.

View Article: PubMed Central - PubMed

Affiliation: Genomics and Synthetic Biology Center, Tsinghua University, Beijing, China.

ABSTRACT
Hospital-acquired infection (HAI) or nosocomial infection is an issue that frequent hospital environment. We believe conventional regulated Petri dish method is insufficient to evaluate HAI. To address this problem, metagenomic sequencing was applied to screen airborne microbes in four rooms of Beijing Hospital. With air-in amount of sampler being setup to one person's respiration quantity, metagenomic sequencing identified huge numbers of species in the rooms which had already qualified widely accepted petridish exposing standard, imposing urgency for new technology. Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies. To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study. It is also potential as a transmissible pathogen real-time modelling system worldwide.

No MeSH data available.


Related in: MedlinePlus

Heatmap of metagenomic recoverd species for each group of microorganisms in four rooms.For bacteria and eukaryote, top 100 abundant species for the heatmap.
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pone.0139044.g003: Heatmap of metagenomic recoverd species for each group of microorganisms in four rooms.For bacteria and eukaryote, top 100 abundant species for the heatmap.

Mentions: The numbers of identified Archaea, Bacteria, Eukaryota and Virus species are 106 (3%), 2170 (52%), 1679 (40%) and 211 (5%), respectively, at ICU; 72 (2%), 1597 (46%), 1759 (50%) and 60 (2%), respectively, at Emergency; 98 (3%), 2261 (65%), 1025 (30%) and 81 (2%), respectively, at Outpatient; 74 (2%), 1826 (51%), 1580 (44%) and 117 (3%), respectively, at Respiratory ICU (Fig 1B and 1C and Table 6). Fig 1A shows the sampling equipment and Fig 1B shows the filter papers. For all four rooms, the proportion of Bacteria and Eukaryota species together exceeds 90%, while that of Archaea and Viruses species together is relatively small. The heatmap of Archaea, Viruses, top 100 Eukaryota species, and top 100 Bacteria species are shown in Fig 3. Relevant excel tables were loaded in S1–S4 Excel Files.


Metagenomic Human Repiratory Air in a Hospital Environment.

Lai YY, Li Y, Lang J, Tong X, Zhang L, Fang J, Xing J, Cai M, Xu H, Deng Y, Xiao F, Tian G - PLoS ONE (2015)

Heatmap of metagenomic recoverd species for each group of microorganisms in four rooms.For bacteria and eukaryote, top 100 abundant species for the heatmap.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592066&req=5

pone.0139044.g003: Heatmap of metagenomic recoverd species for each group of microorganisms in four rooms.For bacteria and eukaryote, top 100 abundant species for the heatmap.
Mentions: The numbers of identified Archaea, Bacteria, Eukaryota and Virus species are 106 (3%), 2170 (52%), 1679 (40%) and 211 (5%), respectively, at ICU; 72 (2%), 1597 (46%), 1759 (50%) and 60 (2%), respectively, at Emergency; 98 (3%), 2261 (65%), 1025 (30%) and 81 (2%), respectively, at Outpatient; 74 (2%), 1826 (51%), 1580 (44%) and 117 (3%), respectively, at Respiratory ICU (Fig 1B and 1C and Table 6). Fig 1A shows the sampling equipment and Fig 1B shows the filter papers. For all four rooms, the proportion of Bacteria and Eukaryota species together exceeds 90%, while that of Archaea and Viruses species together is relatively small. The heatmap of Archaea, Viruses, top 100 Eukaryota species, and top 100 Bacteria species are shown in Fig 3. Relevant excel tables were loaded in S1–S4 Excel Files.

Bottom Line: Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies.To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study.It is also potential as a transmissible pathogen real-time modelling system worldwide.

View Article: PubMed Central - PubMed

Affiliation: Genomics and Synthetic Biology Center, Tsinghua University, Beijing, China.

ABSTRACT
Hospital-acquired infection (HAI) or nosocomial infection is an issue that frequent hospital environment. We believe conventional regulated Petri dish method is insufficient to evaluate HAI. To address this problem, metagenomic sequencing was applied to screen airborne microbes in four rooms of Beijing Hospital. With air-in amount of sampler being setup to one person's respiration quantity, metagenomic sequencing identified huge numbers of species in the rooms which had already qualified widely accepted petridish exposing standard, imposing urgency for new technology. Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies. To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study. It is also potential as a transmissible pathogen real-time modelling system worldwide.

No MeSH data available.


Related in: MedlinePlus