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Metagenomic Human Repiratory Air in a Hospital Environment.

Lai YY, Li Y, Lang J, Tong X, Zhang L, Fang J, Xing J, Cai M, Xu H, Deng Y, Xiao F, Tian G - PLoS ONE (2015)

Bottom Line: Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies.To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study.It is also potential as a transmissible pathogen real-time modelling system worldwide.

View Article: PubMed Central - PubMed

Affiliation: Genomics and Synthetic Biology Center, Tsinghua University, Beijing, China.

ABSTRACT
Hospital-acquired infection (HAI) or nosocomial infection is an issue that frequent hospital environment. We believe conventional regulated Petri dish method is insufficient to evaluate HAI. To address this problem, metagenomic sequencing was applied to screen airborne microbes in four rooms of Beijing Hospital. With air-in amount of sampler being setup to one person's respiration quantity, metagenomic sequencing identified huge numbers of species in the rooms which had already qualified widely accepted petridish exposing standard, imposing urgency for new technology. Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies. To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study. It is also potential as a transmissible pathogen real-time modelling system worldwide.

No MeSH data available.


Related in: MedlinePlus

Number of metagenomic recovered species for each group of microorganisms.a) sampling equipment, the soft tube connected to sampling head allows it to be easily fixed at any position, even could be put before the mouth. b) the sampling filter paper at Nov.6,2013. Left: blank; right: after work for 24 hours. c) Numbers of species and d) proportions of species number in each group of microbes in four rooms.
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pone.0139044.g001: Number of metagenomic recovered species for each group of microorganisms.a) sampling equipment, the soft tube connected to sampling head allows it to be easily fixed at any position, even could be put before the mouth. b) the sampling filter paper at Nov.6,2013. Left: blank; right: after work for 24 hours. c) Numbers of species and d) proportions of species number in each group of microbes in four rooms.

Mentions: Considering future application convenience, we used an air sampler with 0.2μm polytetrafluoroethene (PTFE) filter membrane to intercept all microorganisms in the airflow. It could be regarded as a simplified bioaerosol method. The advantage of this way to routine bioaerosol air sampling [26] or cyclone method [27] is that the filter paper is easy to handle and mail to far away metagenomic lab for sequencing. Fig 1B are two pieces of filter papers, one with 24 hour captured airborne particles and another is blank control. Just the naked eyes we could see some difference. If some of the spraying liquid which can display some malignant pathogen on the filter paper could be technically worked out, monitoring people could then quickly decide whether it is necessary to mail certain samples. Such advantages are quite important while establish a monitoring network. Since most of the sampling sites are actually not only lack metagenomic equipment, but also lack trained personals to handle complex monitoring devices or follow complicated protocols. Therefore, the robust and cost effective filter paper system become a feasible choice. And for a huge monitoring network, individual sampler accuracy is not so important as that of network distribution. Moreover, filter paper does not sacrifice the detection limits. There is a research report which made use of automobile AC filters[28] to sample for 16S rRNA sequence. This is another cost-effective way for establishing a monitoring network. Not quite fit for hospital indoor air due to variation of aircondition distribution in room, but proper for hospital automobile monitoring, one of the HAI transmission routes in Table 1.


Metagenomic Human Repiratory Air in a Hospital Environment.

Lai YY, Li Y, Lang J, Tong X, Zhang L, Fang J, Xing J, Cai M, Xu H, Deng Y, Xiao F, Tian G - PLoS ONE (2015)

Number of metagenomic recovered species for each group of microorganisms.a) sampling equipment, the soft tube connected to sampling head allows it to be easily fixed at any position, even could be put before the mouth. b) the sampling filter paper at Nov.6,2013. Left: blank; right: after work for 24 hours. c) Numbers of species and d) proportions of species number in each group of microbes in four rooms.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592066&req=5

pone.0139044.g001: Number of metagenomic recovered species for each group of microorganisms.a) sampling equipment, the soft tube connected to sampling head allows it to be easily fixed at any position, even could be put before the mouth. b) the sampling filter paper at Nov.6,2013. Left: blank; right: after work for 24 hours. c) Numbers of species and d) proportions of species number in each group of microbes in four rooms.
Mentions: Considering future application convenience, we used an air sampler with 0.2μm polytetrafluoroethene (PTFE) filter membrane to intercept all microorganisms in the airflow. It could be regarded as a simplified bioaerosol method. The advantage of this way to routine bioaerosol air sampling [26] or cyclone method [27] is that the filter paper is easy to handle and mail to far away metagenomic lab for sequencing. Fig 1B are two pieces of filter papers, one with 24 hour captured airborne particles and another is blank control. Just the naked eyes we could see some difference. If some of the spraying liquid which can display some malignant pathogen on the filter paper could be technically worked out, monitoring people could then quickly decide whether it is necessary to mail certain samples. Such advantages are quite important while establish a monitoring network. Since most of the sampling sites are actually not only lack metagenomic equipment, but also lack trained personals to handle complex monitoring devices or follow complicated protocols. Therefore, the robust and cost effective filter paper system become a feasible choice. And for a huge monitoring network, individual sampler accuracy is not so important as that of network distribution. Moreover, filter paper does not sacrifice the detection limits. There is a research report which made use of automobile AC filters[28] to sample for 16S rRNA sequence. This is another cost-effective way for establishing a monitoring network. Not quite fit for hospital indoor air due to variation of aircondition distribution in room, but proper for hospital automobile monitoring, one of the HAI transmission routes in Table 1.

Bottom Line: Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies.To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study.It is also potential as a transmissible pathogen real-time modelling system worldwide.

View Article: PubMed Central - PubMed

Affiliation: Genomics and Synthetic Biology Center, Tsinghua University, Beijing, China.

ABSTRACT
Hospital-acquired infection (HAI) or nosocomial infection is an issue that frequent hospital environment. We believe conventional regulated Petri dish method is insufficient to evaluate HAI. To address this problem, metagenomic sequencing was applied to screen airborne microbes in four rooms of Beijing Hospital. With air-in amount of sampler being setup to one person's respiration quantity, metagenomic sequencing identified huge numbers of species in the rooms which had already qualified widely accepted petridish exposing standard, imposing urgency for new technology. Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies. To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study. It is also potential as a transmissible pathogen real-time modelling system worldwide.

No MeSH data available.


Related in: MedlinePlus