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Expression of Fascin-1 on human lung cancer and paracarcinoma tissue and its relation to clinicopathological characteristics in patients with lung cancer.

Zhao W, Gao J, Wu J, Liu QH, Wang ZG, Li HL, Xing LH - Onco Targets Ther (2015)

Bottom Line: This study set the expression of Fascin-1 in vascular endothelial cells as the positive control, and used phosphate buffered saline (replacing the primary antibodies) as negative control.Differences in Fascin-1 expression between different age groups, clinical stages, and lymph node metastases were statistically significant (P<0.05), while differences in Fascin-1 expression between sexes, tumor stages, and pathological types demonstrated no statistical significance (P>0.05).In lung cancer, Fascin-1 expression was closely related to tumor invasion and metastasis, and the difference in expression of Fascin-1 had a significant effect on the survival time of the lung cancer patients.

View Article: PubMed Central - PubMed

Affiliation: The Third Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People's Republic of China.

ABSTRACT

Background: Lung cancer poses a severe threat to human life. Biomarkers of cancers are helpful in the diagnosis and treatment of patients with cancers. Biomarkers of lung cancers are rare, and thus deserve further research.

Objective: The objective of the present study was to explore the expression of Fascin-1 in human lung cancer and paracarcinoma tissue, its correlation with clinicopathological characteristics in patients with lung cancer, and study the possible relationship between Fascin-1 expression and clinical-biological behavior of lung cancer.

Method: This study used the MaxVision two-step immunohistochemical detection method to detect Fascin-1 expression in 84 of lung cancer and paracarcinoma tissues. This study set the expression of Fascin-1 in vascular endothelial cells as the positive control, and used phosphate buffered saline (replacing the primary antibodies) as negative control.

Result: Of all the 84 lung cancer tissues and paracarcinoma tissues, positive expression of the Fascin-1 protein were detected in 78 cases (92.9%) and 27 cases (32.1%), respectively, and the difference was statistically significant (P<0.05). Differences in Fascin-1 expression between different age groups, clinical stages, and lymph node metastases were statistically significant (P<0.05), while differences in Fascin-1 expression between sexes, tumor stages, and pathological types demonstrated no statistical significance (P>0.05). The survival times of the patients with different Fascin-1 protein-positive expressions in lung cancer tissues were statistically significant (P>0.05), while the survival times of the patients with different Fascin-1 protein-positive expressions in paracarcinoma tissues were not statistically significant (P>0.05).

Conclusion: In lung cancer, Fascin-1 expression was closely related to tumor invasion and metastasis, and the difference in expression of Fascin-1 had a significant effect on the survival time of the lung cancer patients. Therefore, Fascin-1 might be expected to serve as a possible potential biomarker of lung cancer.

No MeSH data available.


Related in: MedlinePlus

Fascin-1 protein-positive expression in the paracarcinoma tissues (DAB ×400).Notes: (A) Bronchial epithelial cells; (B) alveolar epithelial cells.
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f2-ott-8-2571: Fascin-1 protein-positive expression in the paracarcinoma tissues (DAB ×400).Notes: (A) Bronchial epithelial cells; (B) alveolar epithelial cells.

Mentions: Fascin-1 protein was diffused throughout the lung cancer tissues, and dyeing of the cancer cells was stronger than that near the cancer nests. Fascin-1 immunohistochemical dyeing was mainly localized in the cytoplasm and cell membrane of positive cells, with focal shape or diffused yellow granular shape. In all the 84 lung cancer tissues, there were 78 cases (92. 9%) with Fascin-1 protein-positive expression, as shown in Figure 1; in 84 cases of paracarcinoma tissues, there were 27 cases (32.1%) with Fascin-1 protein-positive expression, and the difference was statistically significant (χ2=66.06, P<0.05). In paracarcinoma tissues, Fascin-1 was mainly expressed in the glandular epithelial cells, with membranous type positive expression, as illustrated in Figure 2. Fascin-1 positive staining was visible in vascular endothelial cells of interstitial lung cancer. In the paracarcinoma tissues, capillary endothelial cells and dendritic cells were positive for the expression of Fascin-1, while the bronchial alveolar epithelial cells and pulmonary vascular endothelial cells were negative for the expression of Fascin-1.


Expression of Fascin-1 on human lung cancer and paracarcinoma tissue and its relation to clinicopathological characteristics in patients with lung cancer.

Zhao W, Gao J, Wu J, Liu QH, Wang ZG, Li HL, Xing LH - Onco Targets Ther (2015)

Fascin-1 protein-positive expression in the paracarcinoma tissues (DAB ×400).Notes: (A) Bronchial epithelial cells; (B) alveolar epithelial cells.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592059&req=5

f2-ott-8-2571: Fascin-1 protein-positive expression in the paracarcinoma tissues (DAB ×400).Notes: (A) Bronchial epithelial cells; (B) alveolar epithelial cells.
Mentions: Fascin-1 protein was diffused throughout the lung cancer tissues, and dyeing of the cancer cells was stronger than that near the cancer nests. Fascin-1 immunohistochemical dyeing was mainly localized in the cytoplasm and cell membrane of positive cells, with focal shape or diffused yellow granular shape. In all the 84 lung cancer tissues, there were 78 cases (92. 9%) with Fascin-1 protein-positive expression, as shown in Figure 1; in 84 cases of paracarcinoma tissues, there were 27 cases (32.1%) with Fascin-1 protein-positive expression, and the difference was statistically significant (χ2=66.06, P<0.05). In paracarcinoma tissues, Fascin-1 was mainly expressed in the glandular epithelial cells, with membranous type positive expression, as illustrated in Figure 2. Fascin-1 positive staining was visible in vascular endothelial cells of interstitial lung cancer. In the paracarcinoma tissues, capillary endothelial cells and dendritic cells were positive for the expression of Fascin-1, while the bronchial alveolar epithelial cells and pulmonary vascular endothelial cells were negative for the expression of Fascin-1.

Bottom Line: This study set the expression of Fascin-1 in vascular endothelial cells as the positive control, and used phosphate buffered saline (replacing the primary antibodies) as negative control.Differences in Fascin-1 expression between different age groups, clinical stages, and lymph node metastases were statistically significant (P<0.05), while differences in Fascin-1 expression between sexes, tumor stages, and pathological types demonstrated no statistical significance (P>0.05).In lung cancer, Fascin-1 expression was closely related to tumor invasion and metastasis, and the difference in expression of Fascin-1 had a significant effect on the survival time of the lung cancer patients.

View Article: PubMed Central - PubMed

Affiliation: The Third Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People's Republic of China.

ABSTRACT

Background: Lung cancer poses a severe threat to human life. Biomarkers of cancers are helpful in the diagnosis and treatment of patients with cancers. Biomarkers of lung cancers are rare, and thus deserve further research.

Objective: The objective of the present study was to explore the expression of Fascin-1 in human lung cancer and paracarcinoma tissue, its correlation with clinicopathological characteristics in patients with lung cancer, and study the possible relationship between Fascin-1 expression and clinical-biological behavior of lung cancer.

Method: This study used the MaxVision two-step immunohistochemical detection method to detect Fascin-1 expression in 84 of lung cancer and paracarcinoma tissues. This study set the expression of Fascin-1 in vascular endothelial cells as the positive control, and used phosphate buffered saline (replacing the primary antibodies) as negative control.

Result: Of all the 84 lung cancer tissues and paracarcinoma tissues, positive expression of the Fascin-1 protein were detected in 78 cases (92.9%) and 27 cases (32.1%), respectively, and the difference was statistically significant (P<0.05). Differences in Fascin-1 expression between different age groups, clinical stages, and lymph node metastases were statistically significant (P<0.05), while differences in Fascin-1 expression between sexes, tumor stages, and pathological types demonstrated no statistical significance (P>0.05). The survival times of the patients with different Fascin-1 protein-positive expressions in lung cancer tissues were statistically significant (P>0.05), while the survival times of the patients with different Fascin-1 protein-positive expressions in paracarcinoma tissues were not statistically significant (P>0.05).

Conclusion: In lung cancer, Fascin-1 expression was closely related to tumor invasion and metastasis, and the difference in expression of Fascin-1 had a significant effect on the survival time of the lung cancer patients. Therefore, Fascin-1 might be expected to serve as a possible potential biomarker of lung cancer.

No MeSH data available.


Related in: MedlinePlus