Limits...
Liposomal curcumin inhibits hypoxia-induced angiogenesis after transcatheter arterial embolization in VX2 rabbit liver tumors.

Dai F, Zhang X, Shen W, Chen J, Liu L, Gao G - Onco Targets Ther (2015)

Bottom Line: The levels of HIF-1α and VEGF, and MVD in tumors of liposomal curcumin TAE-treated group were significantly decreased compared to the TAE-treated group (P<0.05).There was a slight decrease in tumor size in the liposomal curcumin TAE-treated group at third-day time points compared to the TAE-treated group; the difference was not statistically significant (P>0.05).A significant correlation between VEGF protein and MVD was noted as well (r=0.519, P=0.027).

View Article: PubMed Central - PubMed

Affiliation: The First College of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Department of Interventional Radiology, The Second Hospital of Nanjing, Medical School, Southeast University, Nanjing, People's Republic of China.

ABSTRACT

Purpose: The purpose of the study is to investigate the inhibition of hypoxia-induced angiogenesis after embolization in VX2 rabbit liver tumors by liposomal curcumin.

Materials and methods: A total of 54 VX2 rabbits were divided into three groups, and each group had three subgroups according to the sacrifice time. The animals in the control group (n=18) underwent sham embolization. Transcatheter arterial embolization (TAE)-treated group (n=18) animals underwent embolization with lipiodol (0.1 mL/kg body weight) and 90-180 µm polyvinyl alcohol (PVA) particles. Liposomal curcumin TAE-treated group (n=18) animals underwent embolization with liposomal curcumin (20 mg/kg body weight) mixed with lipiodol (0.1 mL/kg body weight) and 90-180 µm PVA particles. After embolization, the animals in each subgroup were sacrificed at 6 hours, 24 hours, and 3 days, and the tumor samples were collected. Immunohistochemical staining was performed to evaluate expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) proteins, and microvessel density (MVD). Real-time polymerase chain reaction was performed to examine VEGF mRNA levels.

Results: The levels of HIF-1α and VEGF, and MVD in tumors of liposomal curcumin TAE-treated group were significantly decreased compared to the TAE-treated group (P<0.05). There was a slight decrease in tumor size in the liposomal curcumin TAE-treated group at third-day time points compared to the TAE-treated group; the difference was not statistically significant (P>0.05). The HIF-1α protein correlated considerably with VEGF mRNA (r=0.705, P=0.001) and protein (r=0.655, P=0.003), and MVD (r=0.521, P=0.027). A significant correlation between VEGF protein and MVD was noted as well (r=0.519, P=0.027).

Conclusion: Liposomal curcumin downregulates HIF-1α protein levels and inhibits hypoxia-induced angiogenesis after embolization in VX2 rabbit liver tumors.

No MeSH data available.


Related in: MedlinePlus

Expression of MVD.Notes: In the column graph, compared to the TAE-treated group, a significant decrease and statistical difference in the liposomal curcumin TAE-treated group is indicated by “*”. In the line graph, “*” shows that the mean MVD significantly increased and had the statistical difference between 6 hours and 3 days in the TAE-treated group.Abbreviations: TAE, transcatheter arterial embolization; MVD, microvessel density.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4592055&req=5

f8-ott-8-2601: Expression of MVD.Notes: In the column graph, compared to the TAE-treated group, a significant decrease and statistical difference in the liposomal curcumin TAE-treated group is indicated by “*”. In the line graph, “*” shows that the mean MVD significantly increased and had the statistical difference between 6 hours and 3 days in the TAE-treated group.Abbreviations: TAE, transcatheter arterial embolization; MVD, microvessel density.

Mentions: Microvessels were heterogeneously distributed in the tumors, and the most intense vascularization was observed at the invading edge of tumor margins and in adjacent normal hepatic sinusoids in the TAE-treated group in Figure 7. The line graph in Figure 8 shows that the mean MVD presented an obvious rising (P<0.05) from 6 hours to 3 days in the TAE-treated group. Meanwhile, the column graph revealed that the mean MVD had significantly decreased (P<0.05) in the liposomal curcumin TAE-treated group compared with the TAE-treated group. Although the liposomal curcumin TAE-treated group tumors showed a small increase in mean MVD compared with the control group, the difference did not reach statistical significance (P>0.05).


Liposomal curcumin inhibits hypoxia-induced angiogenesis after transcatheter arterial embolization in VX2 rabbit liver tumors.

Dai F, Zhang X, Shen W, Chen J, Liu L, Gao G - Onco Targets Ther (2015)

Expression of MVD.Notes: In the column graph, compared to the TAE-treated group, a significant decrease and statistical difference in the liposomal curcumin TAE-treated group is indicated by “*”. In the line graph, “*” shows that the mean MVD significantly increased and had the statistical difference between 6 hours and 3 days in the TAE-treated group.Abbreviations: TAE, transcatheter arterial embolization; MVD, microvessel density.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592055&req=5

f8-ott-8-2601: Expression of MVD.Notes: In the column graph, compared to the TAE-treated group, a significant decrease and statistical difference in the liposomal curcumin TAE-treated group is indicated by “*”. In the line graph, “*” shows that the mean MVD significantly increased and had the statistical difference between 6 hours and 3 days in the TAE-treated group.Abbreviations: TAE, transcatheter arterial embolization; MVD, microvessel density.
Mentions: Microvessels were heterogeneously distributed in the tumors, and the most intense vascularization was observed at the invading edge of tumor margins and in adjacent normal hepatic sinusoids in the TAE-treated group in Figure 7. The line graph in Figure 8 shows that the mean MVD presented an obvious rising (P<0.05) from 6 hours to 3 days in the TAE-treated group. Meanwhile, the column graph revealed that the mean MVD had significantly decreased (P<0.05) in the liposomal curcumin TAE-treated group compared with the TAE-treated group. Although the liposomal curcumin TAE-treated group tumors showed a small increase in mean MVD compared with the control group, the difference did not reach statistical significance (P>0.05).

Bottom Line: The levels of HIF-1α and VEGF, and MVD in tumors of liposomal curcumin TAE-treated group were significantly decreased compared to the TAE-treated group (P<0.05).There was a slight decrease in tumor size in the liposomal curcumin TAE-treated group at third-day time points compared to the TAE-treated group; the difference was not statistically significant (P>0.05).A significant correlation between VEGF protein and MVD was noted as well (r=0.519, P=0.027).

View Article: PubMed Central - PubMed

Affiliation: The First College of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Department of Interventional Radiology, The Second Hospital of Nanjing, Medical School, Southeast University, Nanjing, People's Republic of China.

ABSTRACT

Purpose: The purpose of the study is to investigate the inhibition of hypoxia-induced angiogenesis after embolization in VX2 rabbit liver tumors by liposomal curcumin.

Materials and methods: A total of 54 VX2 rabbits were divided into three groups, and each group had three subgroups according to the sacrifice time. The animals in the control group (n=18) underwent sham embolization. Transcatheter arterial embolization (TAE)-treated group (n=18) animals underwent embolization with lipiodol (0.1 mL/kg body weight) and 90-180 µm polyvinyl alcohol (PVA) particles. Liposomal curcumin TAE-treated group (n=18) animals underwent embolization with liposomal curcumin (20 mg/kg body weight) mixed with lipiodol (0.1 mL/kg body weight) and 90-180 µm PVA particles. After embolization, the animals in each subgroup were sacrificed at 6 hours, 24 hours, and 3 days, and the tumor samples were collected. Immunohistochemical staining was performed to evaluate expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) proteins, and microvessel density (MVD). Real-time polymerase chain reaction was performed to examine VEGF mRNA levels.

Results: The levels of HIF-1α and VEGF, and MVD in tumors of liposomal curcumin TAE-treated group were significantly decreased compared to the TAE-treated group (P<0.05). There was a slight decrease in tumor size in the liposomal curcumin TAE-treated group at third-day time points compared to the TAE-treated group; the difference was not statistically significant (P>0.05). The HIF-1α protein correlated considerably with VEGF mRNA (r=0.705, P=0.001) and protein (r=0.655, P=0.003), and MVD (r=0.521, P=0.027). A significant correlation between VEGF protein and MVD was noted as well (r=0.519, P=0.027).

Conclusion: Liposomal curcumin downregulates HIF-1α protein levels and inhibits hypoxia-induced angiogenesis after embolization in VX2 rabbit liver tumors.

No MeSH data available.


Related in: MedlinePlus