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Liposomal curcumin inhibits hypoxia-induced angiogenesis after transcatheter arterial embolization in VX2 rabbit liver tumors.

Dai F, Zhang X, Shen W, Chen J, Liu L, Gao G - Onco Targets Ther (2015)

Bottom Line: The levels of HIF-1α and VEGF, and MVD in tumors of liposomal curcumin TAE-treated group were significantly decreased compared to the TAE-treated group (P<0.05).There was a slight decrease in tumor size in the liposomal curcumin TAE-treated group at third-day time points compared to the TAE-treated group; the difference was not statistically significant (P>0.05).A significant correlation between VEGF protein and MVD was noted as well (r=0.519, P=0.027).

View Article: PubMed Central - PubMed

Affiliation: The First College of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Department of Interventional Radiology, The Second Hospital of Nanjing, Medical School, Southeast University, Nanjing, People's Republic of China.

ABSTRACT

Purpose: The purpose of the study is to investigate the inhibition of hypoxia-induced angiogenesis after embolization in VX2 rabbit liver tumors by liposomal curcumin.

Materials and methods: A total of 54 VX2 rabbits were divided into three groups, and each group had three subgroups according to the sacrifice time. The animals in the control group (n=18) underwent sham embolization. Transcatheter arterial embolization (TAE)-treated group (n=18) animals underwent embolization with lipiodol (0.1 mL/kg body weight) and 90-180 µm polyvinyl alcohol (PVA) particles. Liposomal curcumin TAE-treated group (n=18) animals underwent embolization with liposomal curcumin (20 mg/kg body weight) mixed with lipiodol (0.1 mL/kg body weight) and 90-180 µm PVA particles. After embolization, the animals in each subgroup were sacrificed at 6 hours, 24 hours, and 3 days, and the tumor samples were collected. Immunohistochemical staining was performed to evaluate expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) proteins, and microvessel density (MVD). Real-time polymerase chain reaction was performed to examine VEGF mRNA levels.

Results: The levels of HIF-1α and VEGF, and MVD in tumors of liposomal curcumin TAE-treated group were significantly decreased compared to the TAE-treated group (P<0.05). There was a slight decrease in tumor size in the liposomal curcumin TAE-treated group at third-day time points compared to the TAE-treated group; the difference was not statistically significant (P>0.05). The HIF-1α protein correlated considerably with VEGF mRNA (r=0.705, P=0.001) and protein (r=0.655, P=0.003), and MVD (r=0.521, P=0.027). A significant correlation between VEGF protein and MVD was noted as well (r=0.519, P=0.027).

Conclusion: Liposomal curcumin downregulates HIF-1α protein levels and inhibits hypoxia-induced angiogenesis after embolization in VX2 rabbit liver tumors.

No MeSH data available.


Related in: MedlinePlus

Change in tumor size.Notes: Line graph showed the diameter of the tumor at different time points after treatment. The tumor size had no statistical significance (P>0.05) in the liposomal TAE-treated group compared to the TAE-treated group.Abbreviation: TAE, transcatheter arterial embolization.
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f3-ott-8-2601: Change in tumor size.Notes: Line graph showed the diameter of the tumor at different time points after treatment. The tumor size had no statistical significance (P>0.05) in the liposomal TAE-treated group compared to the TAE-treated group.Abbreviation: TAE, transcatheter arterial embolization.

Mentions: In the control group, the diameter of the tumor revealed a trend of obvious growth from 20.15±0.16 mm to 24.53±0.15 mm after sham embolization. The tumor size slightly decreased in the liposomal curcumin TAE-treated group compared to the TAE-treated group, and the difference was not statistically significant (P>0.05) on the third day after embolization (Figure 3).


Liposomal curcumin inhibits hypoxia-induced angiogenesis after transcatheter arterial embolization in VX2 rabbit liver tumors.

Dai F, Zhang X, Shen W, Chen J, Liu L, Gao G - Onco Targets Ther (2015)

Change in tumor size.Notes: Line graph showed the diameter of the tumor at different time points after treatment. The tumor size had no statistical significance (P>0.05) in the liposomal TAE-treated group compared to the TAE-treated group.Abbreviation: TAE, transcatheter arterial embolization.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592055&req=5

f3-ott-8-2601: Change in tumor size.Notes: Line graph showed the diameter of the tumor at different time points after treatment. The tumor size had no statistical significance (P>0.05) in the liposomal TAE-treated group compared to the TAE-treated group.Abbreviation: TAE, transcatheter arterial embolization.
Mentions: In the control group, the diameter of the tumor revealed a trend of obvious growth from 20.15±0.16 mm to 24.53±0.15 mm after sham embolization. The tumor size slightly decreased in the liposomal curcumin TAE-treated group compared to the TAE-treated group, and the difference was not statistically significant (P>0.05) on the third day after embolization (Figure 3).

Bottom Line: The levels of HIF-1α and VEGF, and MVD in tumors of liposomal curcumin TAE-treated group were significantly decreased compared to the TAE-treated group (P<0.05).There was a slight decrease in tumor size in the liposomal curcumin TAE-treated group at third-day time points compared to the TAE-treated group; the difference was not statistically significant (P>0.05).A significant correlation between VEGF protein and MVD was noted as well (r=0.519, P=0.027).

View Article: PubMed Central - PubMed

Affiliation: The First College of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Department of Interventional Radiology, The Second Hospital of Nanjing, Medical School, Southeast University, Nanjing, People's Republic of China.

ABSTRACT

Purpose: The purpose of the study is to investigate the inhibition of hypoxia-induced angiogenesis after embolization in VX2 rabbit liver tumors by liposomal curcumin.

Materials and methods: A total of 54 VX2 rabbits were divided into three groups, and each group had three subgroups according to the sacrifice time. The animals in the control group (n=18) underwent sham embolization. Transcatheter arterial embolization (TAE)-treated group (n=18) animals underwent embolization with lipiodol (0.1 mL/kg body weight) and 90-180 µm polyvinyl alcohol (PVA) particles. Liposomal curcumin TAE-treated group (n=18) animals underwent embolization with liposomal curcumin (20 mg/kg body weight) mixed with lipiodol (0.1 mL/kg body weight) and 90-180 µm PVA particles. After embolization, the animals in each subgroup were sacrificed at 6 hours, 24 hours, and 3 days, and the tumor samples were collected. Immunohistochemical staining was performed to evaluate expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) proteins, and microvessel density (MVD). Real-time polymerase chain reaction was performed to examine VEGF mRNA levels.

Results: The levels of HIF-1α and VEGF, and MVD in tumors of liposomal curcumin TAE-treated group were significantly decreased compared to the TAE-treated group (P<0.05). There was a slight decrease in tumor size in the liposomal curcumin TAE-treated group at third-day time points compared to the TAE-treated group; the difference was not statistically significant (P>0.05). The HIF-1α protein correlated considerably with VEGF mRNA (r=0.705, P=0.001) and protein (r=0.655, P=0.003), and MVD (r=0.521, P=0.027). A significant correlation between VEGF protein and MVD was noted as well (r=0.519, P=0.027).

Conclusion: Liposomal curcumin downregulates HIF-1α protein levels and inhibits hypoxia-induced angiogenesis after embolization in VX2 rabbit liver tumors.

No MeSH data available.


Related in: MedlinePlus