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Liposomal curcumin inhibits hypoxia-induced angiogenesis after transcatheter arterial embolization in VX2 rabbit liver tumors.

Dai F, Zhang X, Shen W, Chen J, Liu L, Gao G - Onco Targets Ther (2015)

Bottom Line: The levels of HIF-1α and VEGF, and MVD in tumors of liposomal curcumin TAE-treated group were significantly decreased compared to the TAE-treated group (P<0.05).There was a slight decrease in tumor size in the liposomal curcumin TAE-treated group at third-day time points compared to the TAE-treated group; the difference was not statistically significant (P>0.05).A significant correlation between VEGF protein and MVD was noted as well (r=0.519, P=0.027).

View Article: PubMed Central - PubMed

Affiliation: The First College of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Department of Interventional Radiology, The Second Hospital of Nanjing, Medical School, Southeast University, Nanjing, People's Republic of China.

ABSTRACT

Purpose: The purpose of the study is to investigate the inhibition of hypoxia-induced angiogenesis after embolization in VX2 rabbit liver tumors by liposomal curcumin.

Materials and methods: A total of 54 VX2 rabbits were divided into three groups, and each group had three subgroups according to the sacrifice time. The animals in the control group (n=18) underwent sham embolization. Transcatheter arterial embolization (TAE)-treated group (n=18) animals underwent embolization with lipiodol (0.1 mL/kg body weight) and 90-180 µm polyvinyl alcohol (PVA) particles. Liposomal curcumin TAE-treated group (n=18) animals underwent embolization with liposomal curcumin (20 mg/kg body weight) mixed with lipiodol (0.1 mL/kg body weight) and 90-180 µm PVA particles. After embolization, the animals in each subgroup were sacrificed at 6 hours, 24 hours, and 3 days, and the tumor samples were collected. Immunohistochemical staining was performed to evaluate expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) proteins, and microvessel density (MVD). Real-time polymerase chain reaction was performed to examine VEGF mRNA levels.

Results: The levels of HIF-1α and VEGF, and MVD in tumors of liposomal curcumin TAE-treated group were significantly decreased compared to the TAE-treated group (P<0.05). There was a slight decrease in tumor size in the liposomal curcumin TAE-treated group at third-day time points compared to the TAE-treated group; the difference was not statistically significant (P>0.05). The HIF-1α protein correlated considerably with VEGF mRNA (r=0.705, P=0.001) and protein (r=0.655, P=0.003), and MVD (r=0.521, P=0.027). A significant correlation between VEGF protein and MVD was noted as well (r=0.519, P=0.027).

Conclusion: Liposomal curcumin downregulates HIF-1α protein levels and inhibits hypoxia-induced angiogenesis after embolization in VX2 rabbit liver tumors.

No MeSH data available.


Related in: MedlinePlus

The images of the tumor.Notes: (A) CT showed a low-intensity VX2 tumor (arrow) in the left liver lobe. (B) Selective left hepatic artery angiogram before TAE, showing a hyper-vascular tumor (arrow) in the left liver lobe. (C) Selective angiogram after TAE demonstrated no feeding vessel to tumor (arrow). (D) CT showed iodized oil accumulation in the tumor margin (arrow) and gas density after necrosis in embolized tumor center.Abbreviations: CT, computed tomography; TAE, transcatheter arterial embolization.
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f2-ott-8-2601: The images of the tumor.Notes: (A) CT showed a low-intensity VX2 tumor (arrow) in the left liver lobe. (B) Selective left hepatic artery angiogram before TAE, showing a hyper-vascular tumor (arrow) in the left liver lobe. (C) Selective angiogram after TAE demonstrated no feeding vessel to tumor (arrow). (D) CT showed iodized oil accumulation in the tumor margin (arrow) and gas density after necrosis in embolized tumor center.Abbreviations: CT, computed tomography; TAE, transcatheter arterial embolization.

Mentions: CT images showed that solitary VX2 tumors were grown in the left liver lobe of each model (Figure 2A). All targeted tumors were successfully catheterized, and then, the tumor-feeding vessels were obstructed (Figure 2B and C). Figure 2D shows iodized oil accumulation in the tumor margin and gas density after necrosis in embolized tumor center.


Liposomal curcumin inhibits hypoxia-induced angiogenesis after transcatheter arterial embolization in VX2 rabbit liver tumors.

Dai F, Zhang X, Shen W, Chen J, Liu L, Gao G - Onco Targets Ther (2015)

The images of the tumor.Notes: (A) CT showed a low-intensity VX2 tumor (arrow) in the left liver lobe. (B) Selective left hepatic artery angiogram before TAE, showing a hyper-vascular tumor (arrow) in the left liver lobe. (C) Selective angiogram after TAE demonstrated no feeding vessel to tumor (arrow). (D) CT showed iodized oil accumulation in the tumor margin (arrow) and gas density after necrosis in embolized tumor center.Abbreviations: CT, computed tomography; TAE, transcatheter arterial embolization.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592055&req=5

f2-ott-8-2601: The images of the tumor.Notes: (A) CT showed a low-intensity VX2 tumor (arrow) in the left liver lobe. (B) Selective left hepatic artery angiogram before TAE, showing a hyper-vascular tumor (arrow) in the left liver lobe. (C) Selective angiogram after TAE demonstrated no feeding vessel to tumor (arrow). (D) CT showed iodized oil accumulation in the tumor margin (arrow) and gas density after necrosis in embolized tumor center.Abbreviations: CT, computed tomography; TAE, transcatheter arterial embolization.
Mentions: CT images showed that solitary VX2 tumors were grown in the left liver lobe of each model (Figure 2A). All targeted tumors were successfully catheterized, and then, the tumor-feeding vessels were obstructed (Figure 2B and C). Figure 2D shows iodized oil accumulation in the tumor margin and gas density after necrosis in embolized tumor center.

Bottom Line: The levels of HIF-1α and VEGF, and MVD in tumors of liposomal curcumin TAE-treated group were significantly decreased compared to the TAE-treated group (P<0.05).There was a slight decrease in tumor size in the liposomal curcumin TAE-treated group at third-day time points compared to the TAE-treated group; the difference was not statistically significant (P>0.05).A significant correlation between VEGF protein and MVD was noted as well (r=0.519, P=0.027).

View Article: PubMed Central - PubMed

Affiliation: The First College of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China ; Department of Interventional Radiology, The Second Hospital of Nanjing, Medical School, Southeast University, Nanjing, People's Republic of China.

ABSTRACT

Purpose: The purpose of the study is to investigate the inhibition of hypoxia-induced angiogenesis after embolization in VX2 rabbit liver tumors by liposomal curcumin.

Materials and methods: A total of 54 VX2 rabbits were divided into three groups, and each group had three subgroups according to the sacrifice time. The animals in the control group (n=18) underwent sham embolization. Transcatheter arterial embolization (TAE)-treated group (n=18) animals underwent embolization with lipiodol (0.1 mL/kg body weight) and 90-180 µm polyvinyl alcohol (PVA) particles. Liposomal curcumin TAE-treated group (n=18) animals underwent embolization with liposomal curcumin (20 mg/kg body weight) mixed with lipiodol (0.1 mL/kg body weight) and 90-180 µm PVA particles. After embolization, the animals in each subgroup were sacrificed at 6 hours, 24 hours, and 3 days, and the tumor samples were collected. Immunohistochemical staining was performed to evaluate expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) proteins, and microvessel density (MVD). Real-time polymerase chain reaction was performed to examine VEGF mRNA levels.

Results: The levels of HIF-1α and VEGF, and MVD in tumors of liposomal curcumin TAE-treated group were significantly decreased compared to the TAE-treated group (P<0.05). There was a slight decrease in tumor size in the liposomal curcumin TAE-treated group at third-day time points compared to the TAE-treated group; the difference was not statistically significant (P>0.05). The HIF-1α protein correlated considerably with VEGF mRNA (r=0.705, P=0.001) and protein (r=0.655, P=0.003), and MVD (r=0.521, P=0.027). A significant correlation between VEGF protein and MVD was noted as well (r=0.519, P=0.027).

Conclusion: Liposomal curcumin downregulates HIF-1α protein levels and inhibits hypoxia-induced angiogenesis after embolization in VX2 rabbit liver tumors.

No MeSH data available.


Related in: MedlinePlus