Limits...
Nanoparticles of barium induce apoptosis in human phagocytes.

Mores L, França EL, Silva NA, Suchara EA, Honorio-França AC - Int J Nanomedicine (2015)

Bottom Line: Cell viability, superoxide release, intracellular Ca(2+) release, and phagocyte apoptosis were analyzed in the samples.Treatment with barium lowered mononuclear phagocyte viability, increased superoxide release, and reduced intracellular calcium release.In addition, barium increased cell death by apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças, Mato Grosso, Brazil.

ABSTRACT

Purpose: Nutrients and immunological factors of breast milk are essential for newborn growth and the development of their immune system, but this secretion can contain organic and inorganic toxins such as barium. Colostrum contamination with barium is an important issue to investigate because this naturally occurring element is also associated with human activity and industrial pollution. The study evaluated the administration of barium nanoparticles to colostrum, assessing the viability and functional activity of colostral mononuclear phagocytes.

Methods: Colostrum was collected from 24 clinically healthy women (aged 18-35 years). Cell viability, superoxide release, intracellular Ca(2+) release, and phagocyte apoptosis were analyzed in the samples.

Results: Treatment with barium lowered mononuclear phagocyte viability, increased superoxide release, and reduced intracellular calcium release. In addition, barium increased cell death by apoptosis.

Conclusion: These data suggest that nanoparticles of barium in colostrum are toxic to cells, showing the importance of avoiding exposure to this element.

No MeSH data available.


Related in: MedlinePlus

Superoxide release by blood mononuclear phagocytes (mean ± SD, N=6 in each treatment).Notes: Colostral mononuclear cells were treated or not with barium. F=14.8023, P<0.0001. *Differences between phagocytes treated with or without barium.Abbreviations: MN, mononuclear; SD, standard deviation.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4592030&req=5

f1-ijn-10-6021: Superoxide release by blood mononuclear phagocytes (mean ± SD, N=6 in each treatment).Notes: Colostral mononuclear cells were treated or not with barium. F=14.8023, P<0.0001. *Differences between phagocytes treated with or without barium.Abbreviations: MN, mononuclear; SD, standard deviation.

Mentions: MN phagocytes treated with barium chloride exhibited higher superoxide release than spontaneous release (Figure 1) and lower intracellular calcium. Treating colostrum phagocytes with TMB-8 also reduced intracellular Ca2+ release by colostrum phagocytes, which was similar to that of cells treated with barium chloride (Table 2).


Nanoparticles of barium induce apoptosis in human phagocytes.

Mores L, França EL, Silva NA, Suchara EA, Honorio-França AC - Int J Nanomedicine (2015)

Superoxide release by blood mononuclear phagocytes (mean ± SD, N=6 in each treatment).Notes: Colostral mononuclear cells were treated or not with barium. F=14.8023, P<0.0001. *Differences between phagocytes treated with or without barium.Abbreviations: MN, mononuclear; SD, standard deviation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592030&req=5

f1-ijn-10-6021: Superoxide release by blood mononuclear phagocytes (mean ± SD, N=6 in each treatment).Notes: Colostral mononuclear cells were treated or not with barium. F=14.8023, P<0.0001. *Differences between phagocytes treated with or without barium.Abbreviations: MN, mononuclear; SD, standard deviation.
Mentions: MN phagocytes treated with barium chloride exhibited higher superoxide release than spontaneous release (Figure 1) and lower intracellular calcium. Treating colostrum phagocytes with TMB-8 also reduced intracellular Ca2+ release by colostrum phagocytes, which was similar to that of cells treated with barium chloride (Table 2).

Bottom Line: Cell viability, superoxide release, intracellular Ca(2+) release, and phagocyte apoptosis were analyzed in the samples.Treatment with barium lowered mononuclear phagocyte viability, increased superoxide release, and reduced intracellular calcium release.In addition, barium increased cell death by apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças, Mato Grosso, Brazil.

ABSTRACT

Purpose: Nutrients and immunological factors of breast milk are essential for newborn growth and the development of their immune system, but this secretion can contain organic and inorganic toxins such as barium. Colostrum contamination with barium is an important issue to investigate because this naturally occurring element is also associated with human activity and industrial pollution. The study evaluated the administration of barium nanoparticles to colostrum, assessing the viability and functional activity of colostral mononuclear phagocytes.

Methods: Colostrum was collected from 24 clinically healthy women (aged 18-35 years). Cell viability, superoxide release, intracellular Ca(2+) release, and phagocyte apoptosis were analyzed in the samples.

Results: Treatment with barium lowered mononuclear phagocyte viability, increased superoxide release, and reduced intracellular calcium release. In addition, barium increased cell death by apoptosis.

Conclusion: These data suggest that nanoparticles of barium in colostrum are toxic to cells, showing the importance of avoiding exposure to this element.

No MeSH data available.


Related in: MedlinePlus