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Definitive Characterization of CA 19-9 in Resectable Pancreatic Cancer Using a Reference Set of Serum and Plasma Specimens.

Haab BB, Huang Y, Balasenthil S, Partyka K, Tang H, Anderson M, Allen P, Sasson A, Zeh H, Kaul K, Kletter D, Ge S, Bern M, Kwon R, Blasutig I, Srivastava S, Frazier ML, Sen S, Hollingsworth MA, Rinaudo JA, Killary AM, Brand RE - PLoS ONE (2015)

Bottom Line: We gained additional information about the biomarker by comparing two distinct assays.The two CA 9-9 assays agreed well in overall performance but diverged in measurements of individual samples, potentially due to subtle differences in antibody specificity as revealed by glycan array analysis.Thus, the reference set promises be a valuable resource for biomarker validation and comparison, and the CA 19-9 data presented here will be useful for benchmarking and for exploring relationships to CA 19-9.

View Article: PubMed Central - PubMed

Affiliation: Van Andel Research Institute, Grand Rapids, MI, United States of America.

ABSTRACT
The validation of candidate biomarkers often is hampered by the lack of a reliable means of assessing and comparing performance. We present here a reference set of serum and plasma samples to facilitate the validation of biomarkers for resectable pancreatic cancer. The reference set includes a large cohort of stage I-II pancreatic cancer patients, recruited from 5 different institutions, and relevant control groups. We characterized the performance of the current best serological biomarker for pancreatic cancer, CA 19-9, using plasma samples from the reference set to provide a benchmark for future biomarker studies and to further our knowledge of CA 19-9 in early-stage pancreatic cancer and the control groups. CA 19-9 distinguished pancreatic cancers from the healthy and chronic pancreatitis groups with an average sensitivity and specificity of 70-74%, similar to previous studies using all stages of pancreatic cancer. Chronic pancreatitis patients did not show CA 19-9 elevations, but patients with benign biliary obstruction had elevations nearly as high as the cancer patients. We gained additional information about the biomarker by comparing two distinct assays. The two CA 9-9 assays agreed well in overall performance but diverged in measurements of individual samples, potentially due to subtle differences in antibody specificity as revealed by glycan array analysis. Thus, the reference set promises be a valuable resource for biomarker validation and comparison, and the CA 19-9 data presented here will be useful for benchmarking and for exploring relationships to CA 19-9.

No MeSH data available.


Related in: MedlinePlus

Correlation between Assay 1 and Assay 2.The log-transformed CA 19–9 values of all subjects from Assay 1 and Assay 2 were plotted with respect to each other. Each circle indicates an individual patient sample. The trendline is the linear-least squares best fit, and the Spearman’s rank correlation coefficient is 0.74.
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pone.0139049.g002: Correlation between Assay 1 and Assay 2.The log-transformed CA 19–9 values of all subjects from Assay 1 and Assay 2 were plotted with respect to each other. Each circle indicates an individual patient sample. The trendline is the linear-least squares best fit, and the Spearman’s rank correlation coefficient is 0.74.

Mentions: The above analysis shows that CA 19–9 performance is similar between Assay 1 and Assay 2 when evaluated over all patients, but we also wanted to know how the two assays compared for individual patients. A direct comparison of the values obtained for each patient showed major differences for certain patients (Fig 2). The discrepancies between the assays were evenly distributed; in some cases Assay 1 was higher, and in other cases Assay 2 was higher.


Definitive Characterization of CA 19-9 in Resectable Pancreatic Cancer Using a Reference Set of Serum and Plasma Specimens.

Haab BB, Huang Y, Balasenthil S, Partyka K, Tang H, Anderson M, Allen P, Sasson A, Zeh H, Kaul K, Kletter D, Ge S, Bern M, Kwon R, Blasutig I, Srivastava S, Frazier ML, Sen S, Hollingsworth MA, Rinaudo JA, Killary AM, Brand RE - PLoS ONE (2015)

Correlation between Assay 1 and Assay 2.The log-transformed CA 19–9 values of all subjects from Assay 1 and Assay 2 were plotted with respect to each other. Each circle indicates an individual patient sample. The trendline is the linear-least squares best fit, and the Spearman’s rank correlation coefficient is 0.74.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592020&req=5

pone.0139049.g002: Correlation between Assay 1 and Assay 2.The log-transformed CA 19–9 values of all subjects from Assay 1 and Assay 2 were plotted with respect to each other. Each circle indicates an individual patient sample. The trendline is the linear-least squares best fit, and the Spearman’s rank correlation coefficient is 0.74.
Mentions: The above analysis shows that CA 19–9 performance is similar between Assay 1 and Assay 2 when evaluated over all patients, but we also wanted to know how the two assays compared for individual patients. A direct comparison of the values obtained for each patient showed major differences for certain patients (Fig 2). The discrepancies between the assays were evenly distributed; in some cases Assay 1 was higher, and in other cases Assay 2 was higher.

Bottom Line: We gained additional information about the biomarker by comparing two distinct assays.The two CA 9-9 assays agreed well in overall performance but diverged in measurements of individual samples, potentially due to subtle differences in antibody specificity as revealed by glycan array analysis.Thus, the reference set promises be a valuable resource for biomarker validation and comparison, and the CA 19-9 data presented here will be useful for benchmarking and for exploring relationships to CA 19-9.

View Article: PubMed Central - PubMed

Affiliation: Van Andel Research Institute, Grand Rapids, MI, United States of America.

ABSTRACT
The validation of candidate biomarkers often is hampered by the lack of a reliable means of assessing and comparing performance. We present here a reference set of serum and plasma samples to facilitate the validation of biomarkers for resectable pancreatic cancer. The reference set includes a large cohort of stage I-II pancreatic cancer patients, recruited from 5 different institutions, and relevant control groups. We characterized the performance of the current best serological biomarker for pancreatic cancer, CA 19-9, using plasma samples from the reference set to provide a benchmark for future biomarker studies and to further our knowledge of CA 19-9 in early-stage pancreatic cancer and the control groups. CA 19-9 distinguished pancreatic cancers from the healthy and chronic pancreatitis groups with an average sensitivity and specificity of 70-74%, similar to previous studies using all stages of pancreatic cancer. Chronic pancreatitis patients did not show CA 19-9 elevations, but patients with benign biliary obstruction had elevations nearly as high as the cancer patients. We gained additional information about the biomarker by comparing two distinct assays. The two CA 9-9 assays agreed well in overall performance but diverged in measurements of individual samples, potentially due to subtle differences in antibody specificity as revealed by glycan array analysis. Thus, the reference set promises be a valuable resource for biomarker validation and comparison, and the CA 19-9 data presented here will be useful for benchmarking and for exploring relationships to CA 19-9.

No MeSH data available.


Related in: MedlinePlus