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A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica.

Chen J, Zhang D, Zhang C, Xia X, Yin W, Tian Q - PLoS ONE (2015)

Bottom Line: Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines.Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner.Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.

View Article: PubMed Central - PubMed

Affiliation: College of Biological Sciences and technology, Beijing Forestry University, Beijing, China; National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.

ABSTRACT
A PP2C homolog gene was cloned from the drought-treated cDNA library of Populus euphratica. Multiple sequence alignment analysis suggested that the gene is a potential ortholog of HAB1. The expression of this HAB1 ortholog (PeHAB1) was markedly induced by drought and moderately induced by ABA. To characterize its function in ABA signaling, we generated transgenic Arabidopsis thaliana plants overexpressing this gene. Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines. Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner. Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.

No MeSH data available.


Related in: MedlinePlus

Phenotype tests for water loss and stomata opening under ABA treatment.(A) Water loss analysis. Time courses for the percentage of initial fresh weight were recorded from the detached leaves of wild-type controls, L2, and L6. Data are averages ±SE from three independent experiments (n = 10 plants). Asterisks indicate significant difference between transgenic lines and wild-type at different time points (* P<0.05; Student’s t-test). (B) Stomatal photographs of 35S::PeHAB1 transgenic lines and wild-type plants in the presence or absence of ABA. (C) Average stomatal aperture of PeHAB1 overexpression transgenic lines and wild type plants in response to ABA. Data are averages ±SE from three independent experiments (n > 20 stomata). Asterisks indicate significant difference between transgenic lines and wild-type with or without ABA (** P<0.01; Student’s t-test).
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pone.0139466.g005: Phenotype tests for water loss and stomata opening under ABA treatment.(A) Water loss analysis. Time courses for the percentage of initial fresh weight were recorded from the detached leaves of wild-type controls, L2, and L6. Data are averages ±SE from three independent experiments (n = 10 plants). Asterisks indicate significant difference between transgenic lines and wild-type at different time points (* P<0.05; Student’s t-test). (B) Stomatal photographs of 35S::PeHAB1 transgenic lines and wild-type plants in the presence or absence of ABA. (C) Average stomatal aperture of PeHAB1 overexpression transgenic lines and wild type plants in response to ABA. Data are averages ±SE from three independent experiments (n > 20 stomata). Asterisks indicate significant difference between transgenic lines and wild-type with or without ABA (** P<0.01; Student’s t-test).

Mentions: Transpiration rate assays showed that detached leaves of PeHAB1-overexpressing plants lose water more quickly in comparison with those of wild-type plants (Fig 5A; Student’s t-test), indicating a reduced tolerance of transgenic plants to drought stress. To assess whether the drought tolerance reduction was related to an alteration in sensitivity to ABA, we measured the effect of ABA on stomatal aperture. The results showed a significant difference of stomatal aperture between PeHAB1 overexpressing lines and wild-type under 10 μM ABA (Fig 5B and 5C), indicating that ABA-triggered stomatal closure is blocked in transgenic lines, which may result in an enhanced transpiration rate afterwards in PeHAB1 overexpressing lines.


A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica.

Chen J, Zhang D, Zhang C, Xia X, Yin W, Tian Q - PLoS ONE (2015)

Phenotype tests for water loss and stomata opening under ABA treatment.(A) Water loss analysis. Time courses for the percentage of initial fresh weight were recorded from the detached leaves of wild-type controls, L2, and L6. Data are averages ±SE from three independent experiments (n = 10 plants). Asterisks indicate significant difference between transgenic lines and wild-type at different time points (* P<0.05; Student’s t-test). (B) Stomatal photographs of 35S::PeHAB1 transgenic lines and wild-type plants in the presence or absence of ABA. (C) Average stomatal aperture of PeHAB1 overexpression transgenic lines and wild type plants in response to ABA. Data are averages ±SE from three independent experiments (n > 20 stomata). Asterisks indicate significant difference between transgenic lines and wild-type with or without ABA (** P<0.01; Student’s t-test).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4592019&req=5

pone.0139466.g005: Phenotype tests for water loss and stomata opening under ABA treatment.(A) Water loss analysis. Time courses for the percentage of initial fresh weight were recorded from the detached leaves of wild-type controls, L2, and L6. Data are averages ±SE from three independent experiments (n = 10 plants). Asterisks indicate significant difference between transgenic lines and wild-type at different time points (* P<0.05; Student’s t-test). (B) Stomatal photographs of 35S::PeHAB1 transgenic lines and wild-type plants in the presence or absence of ABA. (C) Average stomatal aperture of PeHAB1 overexpression transgenic lines and wild type plants in response to ABA. Data are averages ±SE from three independent experiments (n > 20 stomata). Asterisks indicate significant difference between transgenic lines and wild-type with or without ABA (** P<0.01; Student’s t-test).
Mentions: Transpiration rate assays showed that detached leaves of PeHAB1-overexpressing plants lose water more quickly in comparison with those of wild-type plants (Fig 5A; Student’s t-test), indicating a reduced tolerance of transgenic plants to drought stress. To assess whether the drought tolerance reduction was related to an alteration in sensitivity to ABA, we measured the effect of ABA on stomatal aperture. The results showed a significant difference of stomatal aperture between PeHAB1 overexpressing lines and wild-type under 10 μM ABA (Fig 5B and 5C), indicating that ABA-triggered stomatal closure is blocked in transgenic lines, which may result in an enhanced transpiration rate afterwards in PeHAB1 overexpressing lines.

Bottom Line: Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines.Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner.Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.

View Article: PubMed Central - PubMed

Affiliation: College of Biological Sciences and technology, Beijing Forestry University, Beijing, China; National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.

ABSTRACT
A PP2C homolog gene was cloned from the drought-treated cDNA library of Populus euphratica. Multiple sequence alignment analysis suggested that the gene is a potential ortholog of HAB1. The expression of this HAB1 ortholog (PeHAB1) was markedly induced by drought and moderately induced by ABA. To characterize its function in ABA signaling, we generated transgenic Arabidopsis thaliana plants overexpressing this gene. Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines. Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner. Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.

No MeSH data available.


Related in: MedlinePlus