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A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica.

Chen J, Zhang D, Zhang C, Xia X, Yin W, Tian Q - PLoS ONE (2015)

Bottom Line: Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines.Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner.Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.

View Article: PubMed Central - PubMed

Affiliation: College of Biological Sciences and technology, Beijing Forestry University, Beijing, China; National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.

ABSTRACT
A PP2C homolog gene was cloned from the drought-treated cDNA library of Populus euphratica. Multiple sequence alignment analysis suggested that the gene is a potential ortholog of HAB1. The expression of this HAB1 ortholog (PeHAB1) was markedly induced by drought and moderately induced by ABA. To characterize its function in ABA signaling, we generated transgenic Arabidopsis thaliana plants overexpressing this gene. Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines. Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner. Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.

No MeSH data available.


Related in: MedlinePlus

Real-time PCR analysis of several Clade A PtPP2Cs and PeHAB1 under ABA, NaCl, drought, and cold treatment.(A) Relative gene expression analysis of four Clade A PtPP2Cs under various abiotic stress treatments. (B) Quantitative RT-PCR was performed with PeActin as an internal reference and PeDREB2 as an experimental control. The expression level in untreated leaves was assigned a value of 1. The capital characters represent 200 μM ABA (ABA), 300 mM NaCl (S), dehydration (D), and 4°C cold, respectively. Data are presented as means ± SE from three independent biological replicates.
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pone.0139466.g002: Real-time PCR analysis of several Clade A PtPP2Cs and PeHAB1 under ABA, NaCl, drought, and cold treatment.(A) Relative gene expression analysis of four Clade A PtPP2Cs under various abiotic stress treatments. (B) Quantitative RT-PCR was performed with PeActin as an internal reference and PeDREB2 as an experimental control. The expression level in untreated leaves was assigned a value of 1. The capital characters represent 200 μM ABA (ABA), 300 mM NaCl (S), dehydration (D), and 4°C cold, respectively. Data are presented as means ± SE from three independent biological replicates.

Mentions: Our qPCR analysis indicated that all the Clade A PtPP2C candidates were differentially expressed in response to various abiotic stress treatments. In general, all four genes involved in analysis were induced by drought treatment, and the highest expression level was observed in Potri.009G037300. Besides drought treatment, Potri.009G037300 also showed a high induction under salt treatment, and Potri.010G199600 was seen to be accumulated under cold treatment. The accumulation of Potri.018G060300 increased after both drought and ABA treatments, whereas Potri.008G059200 was the member whose expression was alerted only after drought treatment (Fig 2A).


A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica.

Chen J, Zhang D, Zhang C, Xia X, Yin W, Tian Q - PLoS ONE (2015)

Real-time PCR analysis of several Clade A PtPP2Cs and PeHAB1 under ABA, NaCl, drought, and cold treatment.(A) Relative gene expression analysis of four Clade A PtPP2Cs under various abiotic stress treatments. (B) Quantitative RT-PCR was performed with PeActin as an internal reference and PeDREB2 as an experimental control. The expression level in untreated leaves was assigned a value of 1. The capital characters represent 200 μM ABA (ABA), 300 mM NaCl (S), dehydration (D), and 4°C cold, respectively. Data are presented as means ± SE from three independent biological replicates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592019&req=5

pone.0139466.g002: Real-time PCR analysis of several Clade A PtPP2Cs and PeHAB1 under ABA, NaCl, drought, and cold treatment.(A) Relative gene expression analysis of four Clade A PtPP2Cs under various abiotic stress treatments. (B) Quantitative RT-PCR was performed with PeActin as an internal reference and PeDREB2 as an experimental control. The expression level in untreated leaves was assigned a value of 1. The capital characters represent 200 μM ABA (ABA), 300 mM NaCl (S), dehydration (D), and 4°C cold, respectively. Data are presented as means ± SE from three independent biological replicates.
Mentions: Our qPCR analysis indicated that all the Clade A PtPP2C candidates were differentially expressed in response to various abiotic stress treatments. In general, all four genes involved in analysis were induced by drought treatment, and the highest expression level was observed in Potri.009G037300. Besides drought treatment, Potri.009G037300 also showed a high induction under salt treatment, and Potri.010G199600 was seen to be accumulated under cold treatment. The accumulation of Potri.018G060300 increased after both drought and ABA treatments, whereas Potri.008G059200 was the member whose expression was alerted only after drought treatment (Fig 2A).

Bottom Line: Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines.Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner.Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.

View Article: PubMed Central - PubMed

Affiliation: College of Biological Sciences and technology, Beijing Forestry University, Beijing, China; National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.

ABSTRACT
A PP2C homolog gene was cloned from the drought-treated cDNA library of Populus euphratica. Multiple sequence alignment analysis suggested that the gene is a potential ortholog of HAB1. The expression of this HAB1 ortholog (PeHAB1) was markedly induced by drought and moderately induced by ABA. To characterize its function in ABA signaling, we generated transgenic Arabidopsis thaliana plants overexpressing this gene. Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines. Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner. Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.

No MeSH data available.


Related in: MedlinePlus