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Hyperglycemic Stress Impairs the Stemness Capacity of Kidney Stem Cells in Rats.

Yang G, Jia Y, Li C, Cheng Q, Yue W, Pei X - PLoS ONE (2015)

Bottom Line: However, KSC proliferation, differentiation ability and tolerance to hypoxia were decreased in high-glucose cultures.Taken together, these results suggest the high-glucose microenvironment can damage the reparative ability of KSCs.It may result in a decreased of recovery capability of renal tubules from injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatric Nephrology, Chinese PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing, China.

ABSTRACT
The incidence of acute kidney injury in patients with diabetes is significantly higher than that of patients without diabetes, and may be associated with the poor stemness capacity of kidney stem cells (KSCs) and limited recovery of injured renal tubules. To investigate the effects of hyperglycemic stress on KSC stemness, KSCs were isolated from the rat renal papilla and analyzed for their self-renewal and differentiation abilities. Our results showed that isolated KSCs expressed the mesenchymal stem cell markers N-cadherin, Nestin, CD133, CD29, CD90, and CD73. Moreover, KSCs co-cultured with hypoxia-injured renal tubular epithelial cell (RTECs) induced the expression of the mature epithelial cell marker CK18, suggesting that the KSCs could differentiate into RTECs in vitro. However, KSC proliferation, differentiation ability and tolerance to hypoxia were decreased in high-glucose cultures. Taken together, these results suggest the high-glucose microenvironment can damage the reparative ability of KSCs. It may result in a decreased of recovery capability of renal tubules from injury.

No MeSH data available.


Related in: MedlinePlus

Phenotypic characterization of renal papillary cells.(A) Immunofluorescence analysis for markers of activated fibroblasts (α-SMA, green; Vimentin, red), mesenchymal stem cells (N-cadherin, green), and epithelial cells (CK18, red; E-cadherin, green; ZO-1, red). Nuclei were counterstained with DAPI (blue) (Magnification: ×100). (B) Immunofluorescence staining for the stem cell expression makers Nestin (green) and CD133 (red). Nuclei are stained with DAPI (blue). (Magnification: ×200). (C) Flow cytometry analysis for the MSC markers CD29 (i), CD90 (ii), and CD73 (iii), and the hematopoietic stem cell marker CD45 (iv).
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pone.0139607.g003: Phenotypic characterization of renal papillary cells.(A) Immunofluorescence analysis for markers of activated fibroblasts (α-SMA, green; Vimentin, red), mesenchymal stem cells (N-cadherin, green), and epithelial cells (CK18, red; E-cadherin, green; ZO-1, red). Nuclei were counterstained with DAPI (blue) (Magnification: ×100). (B) Immunofluorescence staining for the stem cell expression makers Nestin (green) and CD133 (red). Nuclei are stained with DAPI (blue). (Magnification: ×200). (C) Flow cytometry analysis for the MSC markers CD29 (i), CD90 (ii), and CD73 (iii), and the hematopoietic stem cell marker CD45 (iv).

Mentions: The cells isolated from the renal papilla exhibited expression of α-SMA and Vimentin (activated fibroblast markers), N-cadherin (mesenchymal cell marker), and Nestin and CD133 (stem cell makers). The epithelial markers expression, including E-cadherin, CK18, and ZO-1, could not be detected (Fig 3A and 3B). Moreover, 99%, 95.8%, and 99.9% of isolated KSCs were positive for the MSC markers CD29, CD90, and CD73, respectively, while only 3.4% were positive for CD45 expression (Fig 3C). This data suggests that the cells isolated from renal papilla may be KSCs.


Hyperglycemic Stress Impairs the Stemness Capacity of Kidney Stem Cells in Rats.

Yang G, Jia Y, Li C, Cheng Q, Yue W, Pei X - PLoS ONE (2015)

Phenotypic characterization of renal papillary cells.(A) Immunofluorescence analysis for markers of activated fibroblasts (α-SMA, green; Vimentin, red), mesenchymal stem cells (N-cadherin, green), and epithelial cells (CK18, red; E-cadherin, green; ZO-1, red). Nuclei were counterstained with DAPI (blue) (Magnification: ×100). (B) Immunofluorescence staining for the stem cell expression makers Nestin (green) and CD133 (red). Nuclei are stained with DAPI (blue). (Magnification: ×200). (C) Flow cytometry analysis for the MSC markers CD29 (i), CD90 (ii), and CD73 (iii), and the hematopoietic stem cell marker CD45 (iv).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592017&req=5

pone.0139607.g003: Phenotypic characterization of renal papillary cells.(A) Immunofluorescence analysis for markers of activated fibroblasts (α-SMA, green; Vimentin, red), mesenchymal stem cells (N-cadherin, green), and epithelial cells (CK18, red; E-cadherin, green; ZO-1, red). Nuclei were counterstained with DAPI (blue) (Magnification: ×100). (B) Immunofluorescence staining for the stem cell expression makers Nestin (green) and CD133 (red). Nuclei are stained with DAPI (blue). (Magnification: ×200). (C) Flow cytometry analysis for the MSC markers CD29 (i), CD90 (ii), and CD73 (iii), and the hematopoietic stem cell marker CD45 (iv).
Mentions: The cells isolated from the renal papilla exhibited expression of α-SMA and Vimentin (activated fibroblast markers), N-cadherin (mesenchymal cell marker), and Nestin and CD133 (stem cell makers). The epithelial markers expression, including E-cadherin, CK18, and ZO-1, could not be detected (Fig 3A and 3B). Moreover, 99%, 95.8%, and 99.9% of isolated KSCs were positive for the MSC markers CD29, CD90, and CD73, respectively, while only 3.4% were positive for CD45 expression (Fig 3C). This data suggests that the cells isolated from renal papilla may be KSCs.

Bottom Line: However, KSC proliferation, differentiation ability and tolerance to hypoxia were decreased in high-glucose cultures.Taken together, these results suggest the high-glucose microenvironment can damage the reparative ability of KSCs.It may result in a decreased of recovery capability of renal tubules from injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatric Nephrology, Chinese PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing, China.

ABSTRACT
The incidence of acute kidney injury in patients with diabetes is significantly higher than that of patients without diabetes, and may be associated with the poor stemness capacity of kidney stem cells (KSCs) and limited recovery of injured renal tubules. To investigate the effects of hyperglycemic stress on KSC stemness, KSCs were isolated from the rat renal papilla and analyzed for their self-renewal and differentiation abilities. Our results showed that isolated KSCs expressed the mesenchymal stem cell markers N-cadherin, Nestin, CD133, CD29, CD90, and CD73. Moreover, KSCs co-cultured with hypoxia-injured renal tubular epithelial cell (RTECs) induced the expression of the mature epithelial cell marker CK18, suggesting that the KSCs could differentiate into RTECs in vitro. However, KSC proliferation, differentiation ability and tolerance to hypoxia were decreased in high-glucose cultures. Taken together, these results suggest the high-glucose microenvironment can damage the reparative ability of KSCs. It may result in a decreased of recovery capability of renal tubules from injury.

No MeSH data available.


Related in: MedlinePlus