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MicroRNA Expression in Myocardial Tissue and Plasma of Patients with End-Stage Heart Failure during LVAD Support: Comparison of Continuous and Pulsatile Devices.

Lok SI, de Jonge N, van Kuik J, van Geffen AJ, Huibers MM, van der Weide P, Siera E, Winkens B, Doevendans PA, de Weger RA, da Costa Martins PA - PLoS ONE (2015)

Bottom Line: Our aim was to analyze cardiac miR expression patterns associated with each type of device, to analyze differences in the regulation of the induced cardiac changes.Circulating miR-21 decreased at 1, 3, and 6 months after LVAD implantation.However, the miR expression patterns do not seem to significantly differ between pf- and cf-LVAD implying that most cardiac changes or clinical outcomes specific to each device do not relate to differences in miR expression levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, University Medical Center, Utrecht, the Netherlands; Department of Pathology, University Medical Center, Utrecht, the Netherlands.

ABSTRACT

Aim: Pulsatile flow left ventricular assist devices (pf-LVADs) are being replaced by continuous flow LVADs (cf-LVADs) in patients with end-stage heart failure (HF). MicroRNAs (miRs) play an important role in the onset and progression of HF. Our aim was to analyze cardiac miR expression patterns associated with each type of device, to analyze differences in the regulation of the induced cardiac changes.

Methods and results: Twenty-six miRs were selected (based on micro-array data and literature studies) and validated in myocardial tissue before and after pf- (n = 17) and cf-LVAD (n = 17) support. Of these, 5 miRs displayed a similar expression pattern among the devices (miR-129*, miR-146a, miR-155, miR-221, miR-222), whereas others only changed significantly during pf-LVAD (miR-let-7i, miR-21, miR-378, miR-378*) or cf-LVAD support (miR-137). In addition, 4 miRs were investigated in plasma of cf-LVAD supported patients (n = 18) and healthy controls (n = 10). Circulating miR-21 decreased at 1, 3, and 6 months after LVAD implantation. MiR-146a, miR-221 and miR-222 showed a fluctuating time pattern post-LVAD.

Conclusion: Our data show a different miR expression pattern after LVAD support, suggesting that differentially expressed miRs are partially responsible for the cardiac morphological and functional changes observed after support. However, the miR expression patterns do not seem to significantly differ between pf- and cf-LVAD implying that most cardiac changes or clinical outcomes specific to each device do not relate to differences in miR expression levels.

No MeSH data available.


Related in: MedlinePlus

Similar changes in miR expression in pulsatile flow (pf-LVAD) and continuous flow LVAD (cf-LVAD).The alteration in miR-expression in myocardial tissue of both pf-LVAD (n = 17) and cf-LVAD (n = 17) patients. miR-129* (a) and miR-146a (b) are up-regulated in post-LVAD in comparison to pre-LVAD, whereas miR- 155 (c), miR-221 (d) and miR-222 (e) are down-regulated in both devices. The asterisk (*) represents p<0.05.
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pone.0136404.g001: Similar changes in miR expression in pulsatile flow (pf-LVAD) and continuous flow LVAD (cf-LVAD).The alteration in miR-expression in myocardial tissue of both pf-LVAD (n = 17) and cf-LVAD (n = 17) patients. miR-129* (a) and miR-146a (b) are up-regulated in post-LVAD in comparison to pre-LVAD, whereas miR- 155 (c), miR-221 (d) and miR-222 (e) are down-regulated in both devices. The asterisk (*) represents p<0.05.

Mentions: Out of the 26 selected miRs (Table A in S1 File), 5 miRs showed a similar pattern during pf-LVAD and cf-LVAD support (tables A-C in S2 File and S3 File). MiR-129* and miR-146a were down-regulated in pre-LVAD supported patients and increased during mechanical support (Fig 1A and 1B). In contrast, miR-155, miR-221 and miR-222 were up-regulated in pre-LVAD and decreased upon implantation (Fig 1C–1E).


MicroRNA Expression in Myocardial Tissue and Plasma of Patients with End-Stage Heart Failure during LVAD Support: Comparison of Continuous and Pulsatile Devices.

Lok SI, de Jonge N, van Kuik J, van Geffen AJ, Huibers MM, van der Weide P, Siera E, Winkens B, Doevendans PA, de Weger RA, da Costa Martins PA - PLoS ONE (2015)

Similar changes in miR expression in pulsatile flow (pf-LVAD) and continuous flow LVAD (cf-LVAD).The alteration in miR-expression in myocardial tissue of both pf-LVAD (n = 17) and cf-LVAD (n = 17) patients. miR-129* (a) and miR-146a (b) are up-regulated in post-LVAD in comparison to pre-LVAD, whereas miR- 155 (c), miR-221 (d) and miR-222 (e) are down-regulated in both devices. The asterisk (*) represents p<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4592005&req=5

pone.0136404.g001: Similar changes in miR expression in pulsatile flow (pf-LVAD) and continuous flow LVAD (cf-LVAD).The alteration in miR-expression in myocardial tissue of both pf-LVAD (n = 17) and cf-LVAD (n = 17) patients. miR-129* (a) and miR-146a (b) are up-regulated in post-LVAD in comparison to pre-LVAD, whereas miR- 155 (c), miR-221 (d) and miR-222 (e) are down-regulated in both devices. The asterisk (*) represents p<0.05.
Mentions: Out of the 26 selected miRs (Table A in S1 File), 5 miRs showed a similar pattern during pf-LVAD and cf-LVAD support (tables A-C in S2 File and S3 File). MiR-129* and miR-146a were down-regulated in pre-LVAD supported patients and increased during mechanical support (Fig 1A and 1B). In contrast, miR-155, miR-221 and miR-222 were up-regulated in pre-LVAD and decreased upon implantation (Fig 1C–1E).

Bottom Line: Our aim was to analyze cardiac miR expression patterns associated with each type of device, to analyze differences in the regulation of the induced cardiac changes.Circulating miR-21 decreased at 1, 3, and 6 months after LVAD implantation.However, the miR expression patterns do not seem to significantly differ between pf- and cf-LVAD implying that most cardiac changes or clinical outcomes specific to each device do not relate to differences in miR expression levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, University Medical Center, Utrecht, the Netherlands; Department of Pathology, University Medical Center, Utrecht, the Netherlands.

ABSTRACT

Aim: Pulsatile flow left ventricular assist devices (pf-LVADs) are being replaced by continuous flow LVADs (cf-LVADs) in patients with end-stage heart failure (HF). MicroRNAs (miRs) play an important role in the onset and progression of HF. Our aim was to analyze cardiac miR expression patterns associated with each type of device, to analyze differences in the regulation of the induced cardiac changes.

Methods and results: Twenty-six miRs were selected (based on micro-array data and literature studies) and validated in myocardial tissue before and after pf- (n = 17) and cf-LVAD (n = 17) support. Of these, 5 miRs displayed a similar expression pattern among the devices (miR-129*, miR-146a, miR-155, miR-221, miR-222), whereas others only changed significantly during pf-LVAD (miR-let-7i, miR-21, miR-378, miR-378*) or cf-LVAD support (miR-137). In addition, 4 miRs were investigated in plasma of cf-LVAD supported patients (n = 18) and healthy controls (n = 10). Circulating miR-21 decreased at 1, 3, and 6 months after LVAD implantation. MiR-146a, miR-221 and miR-222 showed a fluctuating time pattern post-LVAD.

Conclusion: Our data show a different miR expression pattern after LVAD support, suggesting that differentially expressed miRs are partially responsible for the cardiac morphological and functional changes observed after support. However, the miR expression patterns do not seem to significantly differ between pf- and cf-LVAD implying that most cardiac changes or clinical outcomes specific to each device do not relate to differences in miR expression levels.

No MeSH data available.


Related in: MedlinePlus