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Ovarian Transcriptome Analysis of Portunus trituberculatus Provides Insights into Genes Expressed during Phase III and IV Development.

Yang Y, Wang J, Han T, Liu T, Wang C, Xiao J, Mu C, Li R, Yu F, Shi H - PLoS ONE (2015)

Bottom Line: Also, we performed deep sequencing of ovaries in phases III and IV of sexual maturation in P. trituberculatus.Differential analysis of gene expression identified 506 significantly differentially expressed genes, which belong to 20 pathway, transporters, development, transcription factors, metabolism of other amino acids, carbohydrate and lipid, solute carrier family members, and enzymes.Taken together, our study provides the first comprehensive transcriptomic resource for P. trituberculatus ovaries, which will strengthen understanding of the molecular mechanisms underlying the sexual maturation process and advance molecular nutritional studies of P. trituberculatus.

View Article: PubMed Central - PubMed

Affiliation: Fisheries College of Zhejiang Ocean University, Zhoushan, China.

ABSTRACT
Enhancing the production of aquatic animals is crucial for fishery management and aquaculture applications. Ovaries are specialized tissues that play critical roles in producing oocytes and hormones. Significant biochemical changes take place during the sexual maturation of Portunus trituberculatus, but the genetics of this process has not been extensively studied. Transcriptome sequencing can be used to determine gene expression changes within specific periods. In the current study, we used transcriptome sequencing to produce a comprehensive transcript dataset for the ovarian development of P. trituberculatus. Approximately 100 million sequencing reads were generated, and 126,075 transcripts were assembled. Functional annotation of the obtained transcripts revealed important pathways in ovarian development, such as those involving the vitellogenin gene. Also, we performed deep sequencing of ovaries in phases III and IV of sexual maturation in P. trituberculatus. Differential analysis of gene expression identified 506 significantly differentially expressed genes, which belong to 20 pathway, transporters, development, transcription factors, metabolism of other amino acids, carbohydrate and lipid, solute carrier family members, and enzymes. Taken together, our study provides the first comprehensive transcriptomic resource for P. trituberculatus ovaries, which will strengthen understanding of the molecular mechanisms underlying the sexual maturation process and advance molecular nutritional studies of P. trituberculatus.

No MeSH data available.


KEGG map in assemble result.
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pone.0138862.g003: KEGG map in assemble result.

Mentions: To further analyze the possible pathways involved in ovarian maturation, KEGG analysis was applied based on transcriptome results. We mapped 12,219 transcripts to 337 pathways (S2 Table) representing biological systems involved in the transition from phase III to IV in P. trituberculatus ovarian development. As shown in Fig 3, the most abundant pathways represented in the ovary are metabolic (1706 transcripts), biosynthetic pathways of secondary metabolites (437 transcripts), and purine metabolism (260 transcripts). These predicted pathways are likely to be of interest in future investigations by focusing on their functions in P. trituberculatus.


Ovarian Transcriptome Analysis of Portunus trituberculatus Provides Insights into Genes Expressed during Phase III and IV Development.

Yang Y, Wang J, Han T, Liu T, Wang C, Xiao J, Mu C, Li R, Yu F, Shi H - PLoS ONE (2015)

KEGG map in assemble result.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591999&req=5

pone.0138862.g003: KEGG map in assemble result.
Mentions: To further analyze the possible pathways involved in ovarian maturation, KEGG analysis was applied based on transcriptome results. We mapped 12,219 transcripts to 337 pathways (S2 Table) representing biological systems involved in the transition from phase III to IV in P. trituberculatus ovarian development. As shown in Fig 3, the most abundant pathways represented in the ovary are metabolic (1706 transcripts), biosynthetic pathways of secondary metabolites (437 transcripts), and purine metabolism (260 transcripts). These predicted pathways are likely to be of interest in future investigations by focusing on their functions in P. trituberculatus.

Bottom Line: Also, we performed deep sequencing of ovaries in phases III and IV of sexual maturation in P. trituberculatus.Differential analysis of gene expression identified 506 significantly differentially expressed genes, which belong to 20 pathway, transporters, development, transcription factors, metabolism of other amino acids, carbohydrate and lipid, solute carrier family members, and enzymes.Taken together, our study provides the first comprehensive transcriptomic resource for P. trituberculatus ovaries, which will strengthen understanding of the molecular mechanisms underlying the sexual maturation process and advance molecular nutritional studies of P. trituberculatus.

View Article: PubMed Central - PubMed

Affiliation: Fisheries College of Zhejiang Ocean University, Zhoushan, China.

ABSTRACT
Enhancing the production of aquatic animals is crucial for fishery management and aquaculture applications. Ovaries are specialized tissues that play critical roles in producing oocytes and hormones. Significant biochemical changes take place during the sexual maturation of Portunus trituberculatus, but the genetics of this process has not been extensively studied. Transcriptome sequencing can be used to determine gene expression changes within specific periods. In the current study, we used transcriptome sequencing to produce a comprehensive transcript dataset for the ovarian development of P. trituberculatus. Approximately 100 million sequencing reads were generated, and 126,075 transcripts were assembled. Functional annotation of the obtained transcripts revealed important pathways in ovarian development, such as those involving the vitellogenin gene. Also, we performed deep sequencing of ovaries in phases III and IV of sexual maturation in P. trituberculatus. Differential analysis of gene expression identified 506 significantly differentially expressed genes, which belong to 20 pathway, transporters, development, transcription factors, metabolism of other amino acids, carbohydrate and lipid, solute carrier family members, and enzymes. Taken together, our study provides the first comprehensive transcriptomic resource for P. trituberculatus ovaries, which will strengthen understanding of the molecular mechanisms underlying the sexual maturation process and advance molecular nutritional studies of P. trituberculatus.

No MeSH data available.