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Ovarian Transcriptome Analysis of Portunus trituberculatus Provides Insights into Genes Expressed during Phase III and IV Development.

Yang Y, Wang J, Han T, Liu T, Wang C, Xiao J, Mu C, Li R, Yu F, Shi H - PLoS ONE (2015)

Bottom Line: Also, we performed deep sequencing of ovaries in phases III and IV of sexual maturation in P. trituberculatus.Differential analysis of gene expression identified 506 significantly differentially expressed genes, which belong to 20 pathway, transporters, development, transcription factors, metabolism of other amino acids, carbohydrate and lipid, solute carrier family members, and enzymes.Taken together, our study provides the first comprehensive transcriptomic resource for P. trituberculatus ovaries, which will strengthen understanding of the molecular mechanisms underlying the sexual maturation process and advance molecular nutritional studies of P. trituberculatus.

View Article: PubMed Central - PubMed

Affiliation: Fisheries College of Zhejiang Ocean University, Zhoushan, China.

ABSTRACT
Enhancing the production of aquatic animals is crucial for fishery management and aquaculture applications. Ovaries are specialized tissues that play critical roles in producing oocytes and hormones. Significant biochemical changes take place during the sexual maturation of Portunus trituberculatus, but the genetics of this process has not been extensively studied. Transcriptome sequencing can be used to determine gene expression changes within specific periods. In the current study, we used transcriptome sequencing to produce a comprehensive transcript dataset for the ovarian development of P. trituberculatus. Approximately 100 million sequencing reads were generated, and 126,075 transcripts were assembled. Functional annotation of the obtained transcripts revealed important pathways in ovarian development, such as those involving the vitellogenin gene. Also, we performed deep sequencing of ovaries in phases III and IV of sexual maturation in P. trituberculatus. Differential analysis of gene expression identified 506 significantly differentially expressed genes, which belong to 20 pathway, transporters, development, transcription factors, metabolism of other amino acids, carbohydrate and lipid, solute carrier family members, and enzymes. Taken together, our study provides the first comprehensive transcriptomic resource for P. trituberculatus ovaries, which will strengthen understanding of the molecular mechanisms underlying the sexual maturation process and advance molecular nutritional studies of P. trituberculatus.

No MeSH data available.


Gene Ontology classification of assembled gene s in the P. trituberculatus ovary transcriptome.
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pone.0138862.g002: Gene Ontology classification of assembled gene s in the P. trituberculatus ovary transcriptome.

Mentions: A total of 19,183 (52.78%) transcripts of P. trituberculatus were assigned for GO analysis using Blast2GO software. These transcripts were assigned with one or more GO terms, which fell into all three major GO categories, including cellular component, biological process, and molecular function (Fig 2). In the biological process category, cellular (70.15%) and metabolic processes (51.58%) were the most abundant GO terms. For molecular function, GO terms of binding (57.39%) and catalytic activity (38.81%) were highly represented. For cellular components, the most abundant categories were cell (82.22%) and cell part (82.22%).


Ovarian Transcriptome Analysis of Portunus trituberculatus Provides Insights into Genes Expressed during Phase III and IV Development.

Yang Y, Wang J, Han T, Liu T, Wang C, Xiao J, Mu C, Li R, Yu F, Shi H - PLoS ONE (2015)

Gene Ontology classification of assembled gene s in the P. trituberculatus ovary transcriptome.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591999&req=5

pone.0138862.g002: Gene Ontology classification of assembled gene s in the P. trituberculatus ovary transcriptome.
Mentions: A total of 19,183 (52.78%) transcripts of P. trituberculatus were assigned for GO analysis using Blast2GO software. These transcripts were assigned with one or more GO terms, which fell into all three major GO categories, including cellular component, biological process, and molecular function (Fig 2). In the biological process category, cellular (70.15%) and metabolic processes (51.58%) were the most abundant GO terms. For molecular function, GO terms of binding (57.39%) and catalytic activity (38.81%) were highly represented. For cellular components, the most abundant categories were cell (82.22%) and cell part (82.22%).

Bottom Line: Also, we performed deep sequencing of ovaries in phases III and IV of sexual maturation in P. trituberculatus.Differential analysis of gene expression identified 506 significantly differentially expressed genes, which belong to 20 pathway, transporters, development, transcription factors, metabolism of other amino acids, carbohydrate and lipid, solute carrier family members, and enzymes.Taken together, our study provides the first comprehensive transcriptomic resource for P. trituberculatus ovaries, which will strengthen understanding of the molecular mechanisms underlying the sexual maturation process and advance molecular nutritional studies of P. trituberculatus.

View Article: PubMed Central - PubMed

Affiliation: Fisheries College of Zhejiang Ocean University, Zhoushan, China.

ABSTRACT
Enhancing the production of aquatic animals is crucial for fishery management and aquaculture applications. Ovaries are specialized tissues that play critical roles in producing oocytes and hormones. Significant biochemical changes take place during the sexual maturation of Portunus trituberculatus, but the genetics of this process has not been extensively studied. Transcriptome sequencing can be used to determine gene expression changes within specific periods. In the current study, we used transcriptome sequencing to produce a comprehensive transcript dataset for the ovarian development of P. trituberculatus. Approximately 100 million sequencing reads were generated, and 126,075 transcripts were assembled. Functional annotation of the obtained transcripts revealed important pathways in ovarian development, such as those involving the vitellogenin gene. Also, we performed deep sequencing of ovaries in phases III and IV of sexual maturation in P. trituberculatus. Differential analysis of gene expression identified 506 significantly differentially expressed genes, which belong to 20 pathway, transporters, development, transcription factors, metabolism of other amino acids, carbohydrate and lipid, solute carrier family members, and enzymes. Taken together, our study provides the first comprehensive transcriptomic resource for P. trituberculatus ovaries, which will strengthen understanding of the molecular mechanisms underlying the sexual maturation process and advance molecular nutritional studies of P. trituberculatus.

No MeSH data available.