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Identification of Target Genes Involved in Wound Healing Angiogenesis of Endothelial Cells with the Treatment of a Chinese 2-Herb Formula.

Tam JC, Ko CH, Koon CM, Cheng Z, Lok WH, Lau CP, Leung PC, Fung KP, Chan WY, Lau CB - PLoS ONE (2015)

Bottom Line: We had previously demonstrated that a Chinese 2-herb formula (NF3) significantly stimulated angiogenesis of HUVEC in wound healing.The microarray results illustrated that different panels of differentially expressed genes were strictly governed in NF3-treated HUVEC in a time-regulated manner.This study provided concrete scientific evidence in support of the regulatory role of NF3 on endothelial cells involved in wound healing angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

ABSTRACT
Angiogenesis is vitally important in diabetic wound healing. We had previously demonstrated that a Chinese 2-herb formula (NF3) significantly stimulated angiogenesis of HUVEC in wound healing. However, the molecular mechanism has not yet been elucidated. In line with this, global expression profiling of NF3-treated HUVEC was performed so as to assess the regulatory role of NF3 involved in the underlying signaling pathways in wound healing angiogenesis. The microarray results illustrated that different panels of differentially expressed genes were strictly governed in NF3-treated HUVEC in a time-regulated manner. The microarray analysis followed by qRT-PCR and western blotting verification of NF3-treated HUVEC at 6 h revealed the involvement of various genes in diverse biological process, e.g., MAP3K14 in anti-inflammation; SLC5A8 in anti-tumorogenesis; DNAJB7 in protein translation; BIRC5, EPCAM, INSL4, MMP8 and NPR3 in cell proliferation; CXCR7, EPCAM, HAND1 and MMP8 in migration; CXCR7, EPCAM and MMP8 in tubular formation; and BIRC5, CXCR7, EPCAM, HAND1, MMP8 and UBD in angiogenesis. After 16 h incubation of NF3, other sets of genes were shown with differential expression in HUVEC, e.g., IL1RAPL2 and NR1H4 in anti-inflammation; miR28 in anti-tumorogenesis; GRIN1 and LCN1 in anti-oxidation; EPB41 in intracellular signal transduction; PRL and TFAP2A in cell proliferation; miR28, PRL and SCG2 in cell migration; PRL in tubular formation; and miR28, NR1H4 and PRL in angiogenesis. This study provided concrete scientific evidence in support of the regulatory role of NF3 on endothelial cells involved in wound healing angiogenesis.

No MeSH data available.


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The molecular functions of differentially expressed genes in HUVEC after (A) 6 h and (B) 16 h of NF3 treatment.
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pone.0139342.g002: The molecular functions of differentially expressed genes in HUVEC after (A) 6 h and (B) 16 h of NF3 treatment.

Mentions: Genomatix Pathway System (GePS) was applied to classify the differentially expressed genes into molecular functions and biological processes based on an AmiGo gene ontology (GO) database with significance of p < 0.05. As shown in Fig 2A, the most significant molecular function of NF3-treated HUVEC versus control HUVEC at 6 h was toxin binding consisting of GUCY2C and HLA-DQB1 (p = 8.3 x 10−4). Delayed rectifier channel activity included KCNE1 and KCNH1 (p = 1.21 x 10−4). However, only a few genes were grouped into molecular functions with significances from the gene list of NF3-treated HUVEC versus control HUVEC at 6 h. Not much molecular information could be obtained. Surprisingly, more genes in NF3-treated HUVEC versus control HUVEC at 16 h were grouped into different significant molecular functions (Fig 2B). 18 genes were classified into signaling receptor activity (p = 1 x 10−5), while 17 genes belonged to transmembrane signaling receptor activity (p = 1.55 x 10−5). Besides, a number of genes had G-protein coupled receptor activity, signal tranducer activity and receptor activity. The molecular function categorization provided molecular details among the genes, postulating the specific regulation of different panel of differentially expressed genes in NF3-treated HUVEC versus control HUVEC at different time frame.


Identification of Target Genes Involved in Wound Healing Angiogenesis of Endothelial Cells with the Treatment of a Chinese 2-Herb Formula.

Tam JC, Ko CH, Koon CM, Cheng Z, Lok WH, Lau CP, Leung PC, Fung KP, Chan WY, Lau CB - PLoS ONE (2015)

The molecular functions of differentially expressed genes in HUVEC after (A) 6 h and (B) 16 h of NF3 treatment.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591983&req=5

pone.0139342.g002: The molecular functions of differentially expressed genes in HUVEC after (A) 6 h and (B) 16 h of NF3 treatment.
Mentions: Genomatix Pathway System (GePS) was applied to classify the differentially expressed genes into molecular functions and biological processes based on an AmiGo gene ontology (GO) database with significance of p < 0.05. As shown in Fig 2A, the most significant molecular function of NF3-treated HUVEC versus control HUVEC at 6 h was toxin binding consisting of GUCY2C and HLA-DQB1 (p = 8.3 x 10−4). Delayed rectifier channel activity included KCNE1 and KCNH1 (p = 1.21 x 10−4). However, only a few genes were grouped into molecular functions with significances from the gene list of NF3-treated HUVEC versus control HUVEC at 6 h. Not much molecular information could be obtained. Surprisingly, more genes in NF3-treated HUVEC versus control HUVEC at 16 h were grouped into different significant molecular functions (Fig 2B). 18 genes were classified into signaling receptor activity (p = 1 x 10−5), while 17 genes belonged to transmembrane signaling receptor activity (p = 1.55 x 10−5). Besides, a number of genes had G-protein coupled receptor activity, signal tranducer activity and receptor activity. The molecular function categorization provided molecular details among the genes, postulating the specific regulation of different panel of differentially expressed genes in NF3-treated HUVEC versus control HUVEC at different time frame.

Bottom Line: We had previously demonstrated that a Chinese 2-herb formula (NF3) significantly stimulated angiogenesis of HUVEC in wound healing.The microarray results illustrated that different panels of differentially expressed genes were strictly governed in NF3-treated HUVEC in a time-regulated manner.This study provided concrete scientific evidence in support of the regulatory role of NF3 on endothelial cells involved in wound healing angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

ABSTRACT
Angiogenesis is vitally important in diabetic wound healing. We had previously demonstrated that a Chinese 2-herb formula (NF3) significantly stimulated angiogenesis of HUVEC in wound healing. However, the molecular mechanism has not yet been elucidated. In line with this, global expression profiling of NF3-treated HUVEC was performed so as to assess the regulatory role of NF3 involved in the underlying signaling pathways in wound healing angiogenesis. The microarray results illustrated that different panels of differentially expressed genes were strictly governed in NF3-treated HUVEC in a time-regulated manner. The microarray analysis followed by qRT-PCR and western blotting verification of NF3-treated HUVEC at 6 h revealed the involvement of various genes in diverse biological process, e.g., MAP3K14 in anti-inflammation; SLC5A8 in anti-tumorogenesis; DNAJB7 in protein translation; BIRC5, EPCAM, INSL4, MMP8 and NPR3 in cell proliferation; CXCR7, EPCAM, HAND1 and MMP8 in migration; CXCR7, EPCAM and MMP8 in tubular formation; and BIRC5, CXCR7, EPCAM, HAND1, MMP8 and UBD in angiogenesis. After 16 h incubation of NF3, other sets of genes were shown with differential expression in HUVEC, e.g., IL1RAPL2 and NR1H4 in anti-inflammation; miR28 in anti-tumorogenesis; GRIN1 and LCN1 in anti-oxidation; EPB41 in intracellular signal transduction; PRL and TFAP2A in cell proliferation; miR28, PRL and SCG2 in cell migration; PRL in tubular formation; and miR28, NR1H4 and PRL in angiogenesis. This study provided concrete scientific evidence in support of the regulatory role of NF3 on endothelial cells involved in wound healing angiogenesis.

No MeSH data available.


Related in: MedlinePlus