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Identification of Target Genes Involved in Wound Healing Angiogenesis of Endothelial Cells with the Treatment of a Chinese 2-Herb Formula.

Tam JC, Ko CH, Koon CM, Cheng Z, Lok WH, Lau CP, Leung PC, Fung KP, Chan WY, Lau CB - PLoS ONE (2015)

Bottom Line: We had previously demonstrated that a Chinese 2-herb formula (NF3) significantly stimulated angiogenesis of HUVEC in wound healing.The microarray results illustrated that different panels of differentially expressed genes were strictly governed in NF3-treated HUVEC in a time-regulated manner.This study provided concrete scientific evidence in support of the regulatory role of NF3 on endothelial cells involved in wound healing angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

ABSTRACT
Angiogenesis is vitally important in diabetic wound healing. We had previously demonstrated that a Chinese 2-herb formula (NF3) significantly stimulated angiogenesis of HUVEC in wound healing. However, the molecular mechanism has not yet been elucidated. In line with this, global expression profiling of NF3-treated HUVEC was performed so as to assess the regulatory role of NF3 involved in the underlying signaling pathways in wound healing angiogenesis. The microarray results illustrated that different panels of differentially expressed genes were strictly governed in NF3-treated HUVEC in a time-regulated manner. The microarray analysis followed by qRT-PCR and western blotting verification of NF3-treated HUVEC at 6 h revealed the involvement of various genes in diverse biological process, e.g., MAP3K14 in anti-inflammation; SLC5A8 in anti-tumorogenesis; DNAJB7 in protein translation; BIRC5, EPCAM, INSL4, MMP8 and NPR3 in cell proliferation; CXCR7, EPCAM, HAND1 and MMP8 in migration; CXCR7, EPCAM and MMP8 in tubular formation; and BIRC5, CXCR7, EPCAM, HAND1, MMP8 and UBD in angiogenesis. After 16 h incubation of NF3, other sets of genes were shown with differential expression in HUVEC, e.g., IL1RAPL2 and NR1H4 in anti-inflammation; miR28 in anti-tumorogenesis; GRIN1 and LCN1 in anti-oxidation; EPB41 in intracellular signal transduction; PRL and TFAP2A in cell proliferation; miR28, PRL and SCG2 in cell migration; PRL in tubular formation; and miR28, NR1H4 and PRL in angiogenesis. This study provided concrete scientific evidence in support of the regulatory role of NF3 on endothelial cells involved in wound healing angiogenesis.

No MeSH data available.


Related in: MedlinePlus

Supervised principal component analysis (PCA).Microarray hybridizations were performed using total RNA from HUVEC treated with 75 μg/ml NF3 for 16 h. The gene expression profiles of 3 pools of control cells (red) and 3 pools of NF3-treated cells (blue) were compared using PCA. The red or blue ball located at the central position within the cluster represented the mean of gene expression profiles of 3 pools of control or NF3-treated cells. The two distinct clusters of three red and blue balls represented the intra-experimental variation of control cells and NF3-treated cells, respectively. The three-dimensional plot view of gene expression data (including all probe sets on human GeneChip 1.0 ST) were shown with respect to their correlation to the first three principal components.
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pone.0139342.g001: Supervised principal component analysis (PCA).Microarray hybridizations were performed using total RNA from HUVEC treated with 75 μg/ml NF3 for 16 h. The gene expression profiles of 3 pools of control cells (red) and 3 pools of NF3-treated cells (blue) were compared using PCA. The red or blue ball located at the central position within the cluster represented the mean of gene expression profiles of 3 pools of control or NF3-treated cells. The two distinct clusters of three red and blue balls represented the intra-experimental variation of control cells and NF3-treated cells, respectively. The three-dimensional plot view of gene expression data (including all probe sets on human GeneChip 1.0 ST) were shown with respect to their correlation to the first three principal components.

Mentions: Principal component analysis (PCA) is a statistical technique for determining the key variables in a multidimensional data set. It clearly identifies the inter-experimental variations of the differential expression pattern of two groups associated with the validation of intra-experimental variations of the microarray experiment. The two distinct clusters of three red and blue balls represented the intra-experimental variation of control cells and NF3-treated cells, respectively. The PCA results illustrated that there was no overlap in the clusters of three repeated hybridizations (balls in the same color) of the control HUVEC (red) and NF3-treated HUVEC (blue) (Fig 1). The gene expression profile of NF3-treated HUVEC group was well separated from that of control HUVEC group.


Identification of Target Genes Involved in Wound Healing Angiogenesis of Endothelial Cells with the Treatment of a Chinese 2-Herb Formula.

Tam JC, Ko CH, Koon CM, Cheng Z, Lok WH, Lau CP, Leung PC, Fung KP, Chan WY, Lau CB - PLoS ONE (2015)

Supervised principal component analysis (PCA).Microarray hybridizations were performed using total RNA from HUVEC treated with 75 μg/ml NF3 for 16 h. The gene expression profiles of 3 pools of control cells (red) and 3 pools of NF3-treated cells (blue) were compared using PCA. The red or blue ball located at the central position within the cluster represented the mean of gene expression profiles of 3 pools of control or NF3-treated cells. The two distinct clusters of three red and blue balls represented the intra-experimental variation of control cells and NF3-treated cells, respectively. The three-dimensional plot view of gene expression data (including all probe sets on human GeneChip 1.0 ST) were shown with respect to their correlation to the first three principal components.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591983&req=5

pone.0139342.g001: Supervised principal component analysis (PCA).Microarray hybridizations were performed using total RNA from HUVEC treated with 75 μg/ml NF3 for 16 h. The gene expression profiles of 3 pools of control cells (red) and 3 pools of NF3-treated cells (blue) were compared using PCA. The red or blue ball located at the central position within the cluster represented the mean of gene expression profiles of 3 pools of control or NF3-treated cells. The two distinct clusters of three red and blue balls represented the intra-experimental variation of control cells and NF3-treated cells, respectively. The three-dimensional plot view of gene expression data (including all probe sets on human GeneChip 1.0 ST) were shown with respect to their correlation to the first three principal components.
Mentions: Principal component analysis (PCA) is a statistical technique for determining the key variables in a multidimensional data set. It clearly identifies the inter-experimental variations of the differential expression pattern of two groups associated with the validation of intra-experimental variations of the microarray experiment. The two distinct clusters of three red and blue balls represented the intra-experimental variation of control cells and NF3-treated cells, respectively. The PCA results illustrated that there was no overlap in the clusters of three repeated hybridizations (balls in the same color) of the control HUVEC (red) and NF3-treated HUVEC (blue) (Fig 1). The gene expression profile of NF3-treated HUVEC group was well separated from that of control HUVEC group.

Bottom Line: We had previously demonstrated that a Chinese 2-herb formula (NF3) significantly stimulated angiogenesis of HUVEC in wound healing.The microarray results illustrated that different panels of differentially expressed genes were strictly governed in NF3-treated HUVEC in a time-regulated manner.This study provided concrete scientific evidence in support of the regulatory role of NF3 on endothelial cells involved in wound healing angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

ABSTRACT
Angiogenesis is vitally important in diabetic wound healing. We had previously demonstrated that a Chinese 2-herb formula (NF3) significantly stimulated angiogenesis of HUVEC in wound healing. However, the molecular mechanism has not yet been elucidated. In line with this, global expression profiling of NF3-treated HUVEC was performed so as to assess the regulatory role of NF3 involved in the underlying signaling pathways in wound healing angiogenesis. The microarray results illustrated that different panels of differentially expressed genes were strictly governed in NF3-treated HUVEC in a time-regulated manner. The microarray analysis followed by qRT-PCR and western blotting verification of NF3-treated HUVEC at 6 h revealed the involvement of various genes in diverse biological process, e.g., MAP3K14 in anti-inflammation; SLC5A8 in anti-tumorogenesis; DNAJB7 in protein translation; BIRC5, EPCAM, INSL4, MMP8 and NPR3 in cell proliferation; CXCR7, EPCAM, HAND1 and MMP8 in migration; CXCR7, EPCAM and MMP8 in tubular formation; and BIRC5, CXCR7, EPCAM, HAND1, MMP8 and UBD in angiogenesis. After 16 h incubation of NF3, other sets of genes were shown with differential expression in HUVEC, e.g., IL1RAPL2 and NR1H4 in anti-inflammation; miR28 in anti-tumorogenesis; GRIN1 and LCN1 in anti-oxidation; EPB41 in intracellular signal transduction; PRL and TFAP2A in cell proliferation; miR28, PRL and SCG2 in cell migration; PRL in tubular formation; and miR28, NR1H4 and PRL in angiogenesis. This study provided concrete scientific evidence in support of the regulatory role of NF3 on endothelial cells involved in wound healing angiogenesis.

No MeSH data available.


Related in: MedlinePlus