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Characterization of the Leukocyte Response in Acute Vocal Fold Injury.

King SN, Guille J, Thibeault SL - PLoS ONE (2015)

Bottom Line: Surgical injuries, SWC9+/SWC3- cells exhibited hi CD163+ (p<0.05) at 3-days along with upregulation in TNFα and TGFβ1 mRNA compared to 23-days (p<0.05).Higher levels of IL-10 mRNA were found 1-day post-LPS compared to 5-days (p<0.05).Surgical injury elicited a complex phenotype with early TNFα mRNA and CD163+ and persistent TGFβ1 transcript expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurological Surgery, Kentucky Spinal Cord Injury Research Center, University of Louisville, Louisville, Kentucky, United States of America.

ABSTRACT
Macrophages location in the superficial layer of the vocal fold (VF) is not only at the first line of defense, but in a place of physiologic importance to voice quality. This study characterizes and compares macrophage function in two models of acute injury. Porcine VF injuries were created bilaterally by either surgical biopsy or lipopolysaccharide (LPS) (1.5 μg/kg) injection. Animals were sacrificed at 1- or 5-day post LPS or 3-, 7-, or 23-days post-surgical injury (n = 3/time/injury). Flow cytometry characterized immunophenotypes and RT-PCR quantified cytokine gene expression. Uninjured VF were used as controls. Post-surgical and LPS injury, SWC9+/SWC3- cells identified as hi SLA-DR+ (p<0.05) compared to controls along with hi CD16+ expression at 1-day and 3-days respectively compared to all other time points (p<0.05). Surgical injuries, SWC9+/SWC3- cells exhibited hi CD163+ (p<0.05) at 3-days along with upregulation in TNFα and TGFβ1 mRNA compared to 23-days (p<0.05). No measurable changes to IL-12, IFNγ, IL-10, IL-4 mRNA post-surgery. LPS injuries induced upregulation of TNFα, IL-12, IFNγ, IL-10, and IL-4 mRNA at 1- and 5-days compared to controls (p<0.05). Higher levels of IL-10 mRNA were found 1-day post-LPS compared to 5-days (p<0.05). No changes to CD163 or CD80/86 post-LPS were measured. Acute VF injuries revealed a paradigm of markers that appear to associate with each injury. LPS induced a regulatory phenotype indicated by prominent IL-10 mRNA expression. Surgical injury elicited a complex phenotype with early TNFα mRNA and CD163+ and persistent TGFβ1 transcript expression.

No MeSH data available.


Related in: MedlinePlus

Comparison of surface marker expression following surgical injury.SWC9+/SWC3- cells were analyzed for SLA-DR+ (A) in combination with (B) CD16+, (C) CD80/86, or (D) CD163 at days (d) 0, 3, 7, and 23. Data is shown as the mean expression. * represents a statistical significance of p<0.05 when compared to 0-day or all other time points.
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pone.0139260.g005: Comparison of surface marker expression following surgical injury.SWC9+/SWC3- cells were analyzed for SLA-DR+ (A) in combination with (B) CD16+, (C) CD80/86, or (D) CD163 at days (d) 0, 3, 7, and 23. Data is shown as the mean expression. * represents a statistical significance of p<0.05 when compared to 0-day or all other time points.

Mentions: Overall, there was an increased proportion of SWC9+/SWC3- cells producing SLA-DR+ after surgical trauma in comparison to non-injured vocal folds (p<0.0001) (Fig 5A). Significant differences in SLA-DR+ and CD16+, CD80/86+, or CD163+ were found at 3-days post-surgical trauma. CD16+/SLA+ profiles were found to be significantly increased at 3-days in comparison to all other time points (p< 0.001) (Fig 5B). The percent of SWC9+/SWC3- cells expressing CD80/86+/SLA+ was significant after 3-days in comparison to normal control (p< 0.01) (Fig 5C). The expression of CD163+/SLA+ was also increased significantly after 3-days in contrast to 23-days post (p< 0.01) (Fig 5D). No significant changes in surface expression were found between 7- and 23-days post-surgical injuries.


Characterization of the Leukocyte Response in Acute Vocal Fold Injury.

King SN, Guille J, Thibeault SL - PLoS ONE (2015)

Comparison of surface marker expression following surgical injury.SWC9+/SWC3- cells were analyzed for SLA-DR+ (A) in combination with (B) CD16+, (C) CD80/86, or (D) CD163 at days (d) 0, 3, 7, and 23. Data is shown as the mean expression. * represents a statistical significance of p<0.05 when compared to 0-day or all other time points.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591973&req=5

pone.0139260.g005: Comparison of surface marker expression following surgical injury.SWC9+/SWC3- cells were analyzed for SLA-DR+ (A) in combination with (B) CD16+, (C) CD80/86, or (D) CD163 at days (d) 0, 3, 7, and 23. Data is shown as the mean expression. * represents a statistical significance of p<0.05 when compared to 0-day or all other time points.
Mentions: Overall, there was an increased proportion of SWC9+/SWC3- cells producing SLA-DR+ after surgical trauma in comparison to non-injured vocal folds (p<0.0001) (Fig 5A). Significant differences in SLA-DR+ and CD16+, CD80/86+, or CD163+ were found at 3-days post-surgical trauma. CD16+/SLA+ profiles were found to be significantly increased at 3-days in comparison to all other time points (p< 0.001) (Fig 5B). The percent of SWC9+/SWC3- cells expressing CD80/86+/SLA+ was significant after 3-days in comparison to normal control (p< 0.01) (Fig 5C). The expression of CD163+/SLA+ was also increased significantly after 3-days in contrast to 23-days post (p< 0.01) (Fig 5D). No significant changes in surface expression were found between 7- and 23-days post-surgical injuries.

Bottom Line: Surgical injuries, SWC9+/SWC3- cells exhibited hi CD163+ (p<0.05) at 3-days along with upregulation in TNFα and TGFβ1 mRNA compared to 23-days (p<0.05).Higher levels of IL-10 mRNA were found 1-day post-LPS compared to 5-days (p<0.05).Surgical injury elicited a complex phenotype with early TNFα mRNA and CD163+ and persistent TGFβ1 transcript expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurological Surgery, Kentucky Spinal Cord Injury Research Center, University of Louisville, Louisville, Kentucky, United States of America.

ABSTRACT
Macrophages location in the superficial layer of the vocal fold (VF) is not only at the first line of defense, but in a place of physiologic importance to voice quality. This study characterizes and compares macrophage function in two models of acute injury. Porcine VF injuries were created bilaterally by either surgical biopsy or lipopolysaccharide (LPS) (1.5 μg/kg) injection. Animals were sacrificed at 1- or 5-day post LPS or 3-, 7-, or 23-days post-surgical injury (n = 3/time/injury). Flow cytometry characterized immunophenotypes and RT-PCR quantified cytokine gene expression. Uninjured VF were used as controls. Post-surgical and LPS injury, SWC9+/SWC3- cells identified as hi SLA-DR+ (p<0.05) compared to controls along with hi CD16+ expression at 1-day and 3-days respectively compared to all other time points (p<0.05). Surgical injuries, SWC9+/SWC3- cells exhibited hi CD163+ (p<0.05) at 3-days along with upregulation in TNFα and TGFβ1 mRNA compared to 23-days (p<0.05). No measurable changes to IL-12, IFNγ, IL-10, IL-4 mRNA post-surgery. LPS injuries induced upregulation of TNFα, IL-12, IFNγ, IL-10, and IL-4 mRNA at 1- and 5-days compared to controls (p<0.05). Higher levels of IL-10 mRNA were found 1-day post-LPS compared to 5-days (p<0.05). No changes to CD163 or CD80/86 post-LPS were measured. Acute VF injuries revealed a paradigm of markers that appear to associate with each injury. LPS induced a regulatory phenotype indicated by prominent IL-10 mRNA expression. Surgical injury elicited a complex phenotype with early TNFα mRNA and CD163+ and persistent TGFβ1 transcript expression.

No MeSH data available.


Related in: MedlinePlus