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miR-381 Regulates Neural Stem Cell Proliferation and Differentiation via Regulating Hes1 Expression.

Shi X, Yan C, Liu B, Yang C, Nie X, Wang X, Zheng J, Wang Y, Zhu Y - PLoS ONE (2015)

Bottom Line: Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells.Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons.In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.

ABSTRACT
Neural stem cells are self-renewing, multipotent and undifferentiated precursors that retain the capacity for differentiation into both glial (astrocytes and oligodendrocytes) and neuronal lineages. Neural stem cells offer cell-based therapies for neurological disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease and spinal cord injuries. However, their cellular behavior is poorly understood. MicroRNAs (miRNAs) are a class of small noncoding RNAs involved in cell development, proliferation and differentiation through regulating gene expression at post-transcriptional level. The role of miR-381 in the development of neural stem cells remains unknown. In this study, we showed that overexpression of miR-381 promoted neural stem cells proliferation. It induced the neural stem cells differentiation to neurons and inhibited their differentiation to astrocytes. Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells. Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons. In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

No MeSH data available.


Related in: MedlinePlus

miR–381 promoted neural stem cells proliferation and differentiation to neurons by targeting Hes1.(A) The protein expression of Hes1 was measured by Western blot in neural stem cells. (B) CCK–8 was performed to detect the neural stem cells proliferation. (C) The mRNA expression of Hes1 was measured by qRT-PCR in neural stem cells. (D) The protein expression of Hes1 was measured by Western blot in neural stem cells. (E) The mRNA expression of β-tubulin-III was measured by qRT-PCR in neural stem cells. (F)The protein expression of β-tubulin-III was measured by Western blot in neural stem cells. (G) The mRNA expression of GFAP was measured by qRT-PCR in neural stem cells. (F)The protein expression of GFAP was measured by Western blot in neural stem cells.**p<0.01 and ***p<0.001.
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pone.0138973.g006: miR–381 promoted neural stem cells proliferation and differentiation to neurons by targeting Hes1.(A) The protein expression of Hes1 was measured by Western blot in neural stem cells. (B) CCK–8 was performed to detect the neural stem cells proliferation. (C) The mRNA expression of Hes1 was measured by qRT-PCR in neural stem cells. (D) The protein expression of Hes1 was measured by Western blot in neural stem cells. (E) The mRNA expression of β-tubulin-III was measured by qRT-PCR in neural stem cells. (F)The protein expression of β-tubulin-III was measured by Western blot in neural stem cells. (G) The mRNA expression of GFAP was measured by qRT-PCR in neural stem cells. (F)The protein expression of GFAP was measured by Western blot in neural stem cells.**p<0.01 and ***p<0.001.

Mentions: We confirmed thatHes1 vector can promote the expression of Hes1 in neural stem cells (Fig 6A). Overexpression of Hes1 can impair miR-381-induced promotion of neural stem cells proliferation (Fig 6B). Ectopic expression of Hes1 inhibited miR-381-induced nestin mRNA and protein expression in neural stem cells (Fig 6C and 6D). Hes1 overexpression can repress miR-381-induced β-tubulin III mRNA and protein expression in neural stem cells (Fig 6E and 6F). Hes1 overexpression can promoted miR-381-inhibited GFAP mRNA and protein expression in neural stem cells (Fig 6G and 6H).


miR-381 Regulates Neural Stem Cell Proliferation and Differentiation via Regulating Hes1 Expression.

Shi X, Yan C, Liu B, Yang C, Nie X, Wang X, Zheng J, Wang Y, Zhu Y - PLoS ONE (2015)

miR–381 promoted neural stem cells proliferation and differentiation to neurons by targeting Hes1.(A) The protein expression of Hes1 was measured by Western blot in neural stem cells. (B) CCK–8 was performed to detect the neural stem cells proliferation. (C) The mRNA expression of Hes1 was measured by qRT-PCR in neural stem cells. (D) The protein expression of Hes1 was measured by Western blot in neural stem cells. (E) The mRNA expression of β-tubulin-III was measured by qRT-PCR in neural stem cells. (F)The protein expression of β-tubulin-III was measured by Western blot in neural stem cells. (G) The mRNA expression of GFAP was measured by qRT-PCR in neural stem cells. (F)The protein expression of GFAP was measured by Western blot in neural stem cells.**p<0.01 and ***p<0.001.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4591969&req=5

pone.0138973.g006: miR–381 promoted neural stem cells proliferation and differentiation to neurons by targeting Hes1.(A) The protein expression of Hes1 was measured by Western blot in neural stem cells. (B) CCK–8 was performed to detect the neural stem cells proliferation. (C) The mRNA expression of Hes1 was measured by qRT-PCR in neural stem cells. (D) The protein expression of Hes1 was measured by Western blot in neural stem cells. (E) The mRNA expression of β-tubulin-III was measured by qRT-PCR in neural stem cells. (F)The protein expression of β-tubulin-III was measured by Western blot in neural stem cells. (G) The mRNA expression of GFAP was measured by qRT-PCR in neural stem cells. (F)The protein expression of GFAP was measured by Western blot in neural stem cells.**p<0.01 and ***p<0.001.
Mentions: We confirmed thatHes1 vector can promote the expression of Hes1 in neural stem cells (Fig 6A). Overexpression of Hes1 can impair miR-381-induced promotion of neural stem cells proliferation (Fig 6B). Ectopic expression of Hes1 inhibited miR-381-induced nestin mRNA and protein expression in neural stem cells (Fig 6C and 6D). Hes1 overexpression can repress miR-381-induced β-tubulin III mRNA and protein expression in neural stem cells (Fig 6E and 6F). Hes1 overexpression can promoted miR-381-inhibited GFAP mRNA and protein expression in neural stem cells (Fig 6G and 6H).

Bottom Line: Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells.Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons.In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.

ABSTRACT
Neural stem cells are self-renewing, multipotent and undifferentiated precursors that retain the capacity for differentiation into both glial (astrocytes and oligodendrocytes) and neuronal lineages. Neural stem cells offer cell-based therapies for neurological disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease and spinal cord injuries. However, their cellular behavior is poorly understood. MicroRNAs (miRNAs) are a class of small noncoding RNAs involved in cell development, proliferation and differentiation through regulating gene expression at post-transcriptional level. The role of miR-381 in the development of neural stem cells remains unknown. In this study, we showed that overexpression of miR-381 promoted neural stem cells proliferation. It induced the neural stem cells differentiation to neurons and inhibited their differentiation to astrocytes. Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells. Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons. In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

No MeSH data available.


Related in: MedlinePlus